Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli
Open accessAbstract
Aviation fuels are an important target of biofuels research due to their high market demand and competitive price. Isoprenoids have been demonstrated as good feedstocks for advanced renewable jet fuels with high energy density, high heat of combustion, and excellent cold-weather performance. In particular, sesquiterpene compounds (C 15 ), such as farnesene and bisabolene, have been identified as promising jet fuel candidates. In this study, we explored three sesquiterpenes—epi-isozizaene, pentalenene and α-isocomene—as novel jet fuel precursors. We performed a computational analysis to calculate the energy of combustion of these sesquiterpenes and found that their specific energies are comparable to commercial jet fuel A-1. Through heterologous MVA pathway expression and promoter engineering, we produced 727.9 mg/L epi-isozizaene, 780.3 mg/L pentalenene and 77.5 mg/L α-isocomene in Escherichia coli and 344 mg/L pentalenene in Saccharomyces cerevisiae . We also introduced a dynamic autoinduction system using previously identified FPP-responsive promoters for inducer-free production and managed to achieve comparable amounts of each compound. We produced tricyclic sesquiterpenes epi-isozizaene, pentalenene and α-isocomene, promising jet fuel feedstocks at high production titers, providing novel, sustainable alternatives to petroleum-based jet fuels.
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Hydrogenation of β-farnesene produces farnesane which is an alkane which has displayed potential as a high energy density alternative fuel option.
“Hydrogenation of β-farnesene produces farnesane which is an alkane which has displayed potential as a high energy density alternative fuel option [139].”
From Investigation of Biofuel as a Potential Renewable Energy Source (2021) · cites this paper as [139]
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Investigation of Biofuel as a Potential Renewable Energy Source
Khan, M. A. H., Bonifacio, S., Clowes, J., Foulds, A., Holland, R., Matthews, J. C., Percival, C. J., & Shallcross, D. E. (2021) · Atmosphere · doi:10.3390/atmos12101289 · Cited by 140 · see citing works
- Authors
- Liu, C.-L., Tian, T., Alonso-Gutierrez, J., Garabedian, B., Wang, S., Baidoo, E. E. K., Benites, V., Chen, Y., Petzold, C. J., Adams, P. D., Keasling, J. D., Tan, T., & Lee, T. S.
- Affiliations
- Lawrence Berkeley National Laboratory (US)
- Beijing University of Chemical Technology (CN)
- Corresponding author
- Tan, Tianwei
- Lee, Taek Soon
- ORCID
- Venue
- Biotechnology for Biofuels
- Published
- 2018-10-20
- Volume
- 11
- Issue
- 1
- Pages
- 285-285
- ISSN
- 1754-6834 (ISSN-L)
- PMC
- PMC6195743
- OpenAlex
- W2897769271
- Cited by
- 70
- References
- 46
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 12 · 2023: 11 · 2022: 11 · 2021: 11 · 2020: 8 · 2019: 3
- FWCI
- 1.96
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Biotechnology for Biofuels (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion, cc-by)
- Funders
- Office of Science (DE-AC02-05CH11231)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
- Keywords
- renewable jet fuel, sesquiterpenes, pentalenene, terpenoids, Escherichia coli, Saccharomyces cerevisiae, promoter engineering, autoinduction, aviation biofuels
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