The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
An Integrated Device for Monitoring Time‐Dependent in vitro Expression From Single Genes in Picolitre Droplets
Abstract
Microdroplets have great potential for high‐throughput biochemical screening. We report the design of an integrated microfluidic device for droplet formation, incubation and screening. Picolitre water‐in‐oil droplets can be stored in a reservoir that contains ∼10 6 droplets. In this reservoir droplets are stable for at least 6 h, which gives an extended timescale for biochemical experiments. We demonstrate the utility of the system by following the in vitro expression of green fluorescent protein. The high efficiency allows protein expression from a single molecule of DNA template, creating “monoclonal droplets” in which genotype and phenotype are combined in one emulsion compartment.
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Cites
Solvent Compatibility of Poly(dimethylsiloxane)-Based Microfluidic Devices
Lee, J. N., Park, C., & Whitesides, G. M. (2003) · Analytical Chemistry · doi:10.1021/ac0346712 · Cited by 2,643
A vesicle bioreactor as a step toward an artificial cell assembly
Noireaux, V., & Libchaber, A. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0408236101 · Cited by 1,152
Millisecond Kinetics on a Microfluidic Chip Using Nanoliters of Reagents
Song, H., & Ismagilov, R. F. (2003) · Journal of the American Chemical Society · doi:10.1021/ja0354566 · Cited by 693
Cited by
Seemann, R., Brinkmann, M., Pfohl, T., & Herminghaus, S. (2012) · Reports on Progress in Physics · doi:10.1088/0034-4885/75/1/016601 · Cited by 1,049
Cell-free gene expression: an expanded repertoire of applications
Silverman, A. D., Karim, A. S., & Jewett, M. C. (2020) · Nature Reviews Genetics · doi:10.1038/s41576-019-0186-3 · Cited by 593
Droplet microfluidics: recent developments and future applications
Casadevall i Solvas, X., & deMello, A. (2011) · Chem. Commun. · doi:10.1039/c0cc02474k · Cited by 353
Mazutis, L., Araghi, A. F., Miller, O. J., Baret, J.-C., Frenz, L., Janoshazi, A., Taly, V., Miller, B. J., Hutchison, J. B., Link, D., et al. (2009) · Analytical Chemistry · doi:10.1021/ac900403z · Cited by 263
Concentration gradient generation methods based on microfluidic systems
Wang, X., Liu, Z., & Pang, Y. (2017) · RSC Advances · doi:10.1039/c7ra04494a · Cited by 219
Metadata
- Authors
- Courtois, F., Olguin, L. F., Whyte, G., Bratton, D., Huck, W. T. S., Abell, C., & Hollfelder, F.
- Venue
- ChemBioChem
- Publisher
- Wiley
- Published
- 2008-02-08
- Volume
- 9
- Issue
- 3
- Pages
- 439-446
- ISSN
- 1439-4227 (ISSN-L)
- 1439-7633
- OpenAlex
- W2132454057
- Cited by
- 183
- References
- 3
- FWCI
- 12.15
- Type
- journal article
- Language
- en
- Open access
- closed
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- Courtois, F., Olguin, L. F., Whyte, G., Bratton, D., Huck, W. T. S., Abell, C., & Hollfelder, F. (2008). An Integrated Device for Monitoring Time‐Dependent in vitro Expression From Single Genes in Picolitre Droplets. ChemBioChem. https://doi.org/10.1002/cbic.200700536
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