The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Droplet-Based Microfluidic Systems for High-Throughput Single DNA Molecule Isothermal Amplification and Analysis
Abstract
We have developed a method for high-throughput isothermal amplification of single DNA molecules in a droplet-based microfluidic system. DNA amplification in droplets was analyzed using an intercalating fluorochrome, allowing fast and accurate "digital" quantification of the template DNA based on the Poisson distribution of DNA molecules in droplets. The clonal amplified DNA in each 2 pL droplet was further analyzed by measuring the enzymatic activity of the encoded proteins after fusion with a 15 pL droplet containing an in vitro translation system.
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Cites
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Self-citationMicrofluidic methods for generating continuous droplet streams
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Electric Control of Droplets in Microfluidic Devices
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Self-citationMicrofluidic Digital PCR Enables Multigene Analysis of Individual Environmental Bacteria
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Cited by
Quan, P.-L., Sauzade, M., & Brouzes, E. (2018) · Sensors · doi:10.3390/s18041271 · Cited by 664
Droplet microfluidics: recent developments and future applications
Casadevall i Solvas, X., & deMello, A. (2011) · Chem. Commun. · doi:10.1039/c0cc02474k · Cited by 353
Shen, F., Du, W., Kreutz, J. E., Fok, A., & Ismagilov, R. F. (2010) · Lab on a Chip · doi:10.1039/c004521g · Cited by 295
Shen, F., Davydova, E. K., Du, W., Kreutz, J. E., Piepenburg, O., & Ismagilov, R. F. (2011) · Analytical Chemistry · doi:10.1021/ac200247e · Cited by 240
Transient compartmentalization of RNA replicators prevents extinction due to parasites
Matsumura, S., Kun, Á., Ryckelynck, M., Coldren, F., Szilágyi, A., Jossinet, F., Rick, C., Nghe, P., Szathmáry, E., & Griffiths, A. D. (2016) · Science · doi:10.1126/science.aag1582 · Cited by 162
Self-citation
Metadata
- Authors
- Mazutis, L., Araghi, A. F., Miller, O. J., Baret, J.-C., Frenz, L., Janoshazi, A., Taly, V., Miller, B. J., Hutchison, J. B., Link, D., Griffiths, A. D., & Ryckelynck, M.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-05-18
- Volume
- 81
- Issue
- 12
- Pages
- 4813-4821
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2074905581
- Cited by
- 263
- References
- 26
- FWCI
- 12.23
- Type
- journal article
- Language
- en
- Open access
- closed
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- Mazutis, L., Araghi, A. F., Miller, O. J., Baret, J.-C., Frenz, L., Janoshazi, A., Taly, V., Miller, B. J., Hutchison, J. B., Link, D., Griffiths, A. D., & Ryckelynck, M. (2009). Droplet-Based Microfluidic Systems for High-Throughput Single DNA Molecule Isothermal Amplification and Analysis. Analytical Chemistry. https://doi.org/10.1021/ac900403z
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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