Cited by 62Origins and Evolution of LifeRNA and protein synthesis mechanismsDNA and Nucleic Acid Chemistry

Generation of RNA Molecules by a Base‐Catalysed Click‐Like Reaction

Costanzo, Giovanna, Saladino, Raffaele, Botta, Giorgia, Giorgi, Alessandra, Scipioni, Anita, Pino, Samanta, Di Mauro, Ernesto·2012·ChemBioChem

Abstract

The problem of the abiotic origin of RNA from prebiotically plausible compounds remains unsolved. As a potential partial solution, we report the spontaneous polymerization of 3′,5′‐cyclic GMP in water, in formamide, in dimethylformamide, and (in water) in the presence of a Brønsted base such as 1,8‐diazabicycloundec‐7‐ene. The reaction is untemplated, does not require enzymatic activities, is thermodynamically favoured and selectively yields 3′,5′‐bonded ribopolymers containing as many as 25 nucleotides. We propose a reaction pathway on the basis of 1) the measured stacking of the 3′,5′‐cyclic monomers, 2) the activation by Brønsted bases, 3) the determination (by MALDI‐TOF mass spectrometry, by 31 P NMR, and by specific ribonucleases) of the molecular species produced. The reaction pathway has several of the attributes of a click‐like reaction.

Integrity signals

Registry

Crossref · 10.1002/cbic.201200068

Cites

49 of 49 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Costanzo, G., Saladino, R., Botta, G., Giorgi, A., Scipioni, A., Pino, S., & Di Mauro, E.
Affiliations
  • Institute of Molecular Biology and Pathology (IT)
  • Università degli Studi della Tuscia (IT)
  • Sapienza University of Rome (IT)
  • Istituto Pasteur (IT)
Venue
ChemBioChem
Publisher
Wiley
Published
2012-03-30
Volume
13
Issue
7
Pages
999-1008
ISSN
  • 1439-4227 (ISSN-L)
  • 1439-7633
OpenAlex
W2018142855
Cited by
62
References
49
Citations by year
2026: 1 · 2025: 2 · 2024: 3 · 2023: 4 · 2022: 2 · 2021: 5 · 2020: 8 · 2019: 4 · 2018: 5 · 2017: 4 · 2016: 6 · 2015: 7 · 2014: 2 · 2013: 5 · 2012: 4
FWCI
3.15
Type
journal article
Language
en
Open access
closed
Locations
  • ChemBioChem (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • UnitusOpen (Tuscia University) (submittedVersion)
  • IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
Topics
  • Physical Sciences › Physics and Astronomy › Astronomy and Astrophysics › Origins and Evolution of Life
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords
Brønsted base catalysis, nucleotide stacking, prebiotic chemistry, nucleotide activation
MeSH terms
Catalysis, Magnetic Resonance Spectroscopy, RNA, Molecular Structure, Click Chemistry, Polymerization

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review