The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Design of a phosphinate-based fluorescent probe for superoxide detection in mouse peritoneal macrophages
Abstract
3′,6′‐Bis(diphenylphosphinyl)fluorescein (PF‐1) was synthesized as a highly selective and sensitive fluorescent probe for imaging O 2.− in living cells. The design strategy for the probe was based on the nucleophilic mechanism of O 2.− to mediate deprotection of this probe to give fluorescein. Upon reaction with O 2.−, the probe exhibits a strong fluorescence response and high selectivity for O 2.− over other reactive oxygen species and some biological compounds. The phosphinate‐based probe, as a new fluorescent reagent, is cell‐permeable and can detect micromolar changes of O 2.− concentrations by using confocal microscopy in living cells. The unique combination of good selectivity, high sensitivity, good water solubility, and rapid reactivity establishes the potential value of the probe for facilitating investigations of the generation, metabolism, and mechanisms of superoxide‐mediated cellular homeostasis and injury.
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“The fluorescent probes (c) and (d) were designed by Tang and co-workers, and their functions are based on the nucleophilic property of O2•− which is involved in the deprotection of the diphenylphosphinate residues attached to the fluorescein probe [166] (c), Figure 67, or the naphthofluorescein probe [167] (d).”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [166]
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Cites
Principles of Fluorescence Spectroscopy
Lakowicz, J. R. (1999) · Springer eBooks · doi:10.1007/978-1-4757-3061-6 · Cited by 26,948
Oxidants, oxidative stress and the biology of ageing
Finkel, T., & Holbrook, N. J. (2000) · Nature · doi:10.1038/35041687 · Cited by 9,413
Pathways towards and Away from Alzheimer’s Disease
Mattson, M. P. (2004) · Nature · doi:10.1038/nature02621 · Cited by 3,052
Halliwell, B., & Whiteman, M. (2004) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0705776 · Cited by 2,238
Genetically encoded fluorescent indicator for intracellular hydrogen peroxide
Belousov, V. V., Fradkov, A. F., Lukyanov, K. A., Staroverov, D. B., Shakhbazov, K., Terskikh, A. V., & Lukyanov, S. (2006) · Nature Methods · doi:10.1038/nmeth866 · Cited by 1,286
Methods of Detection of Vascular Reactive Species
Tarpey, M. M., & Fridovich, I. (2001) · Circulation Research · doi:10.1161/hh1501.094365 · Cited by 554
Critical evaluation of the use of hydroethidine as a measure of superoxide anion radical
Benov, L., Sztejnberg, L., & Fridovich, I. (1998) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(98)00163-4 · Cited by 495
Detection of superoxide generated by endothelial cells.
Rosen, G. M., & Freeman, B. Α. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.23.7269 · Cited by 402
Reactive Oxygen Species Produced by NAD(P)H Oxidase Inhibit Apoptosis in Pancreatic Cancer Cells
Vaquero, E. C., Edderkaoui, M., Pandol, S. J., Gukovsky, I., & Gukovskaya, A. S. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m400078200 · Cited by 366
Peters, O., Back, T., Lindauer, U., Busch, C., Megow, D., Dreier, J. P., & Dirnagl, U. (1998) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1097/00004647-199802000-00011 · Cited by 298
Detection of Superoxide in Vascular Tissue
Münzel, T. F., Afanasʹev, I. B., Kleschyov, A. L., & Harrison, D. G. (2002) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000034022.11764.ec · Cited by 281
A design of fluorescent probes for superoxide based on a nonredox mechanism
Maeda, H., Yamamoto, K., Nomura, Y., Kohno, I., Hafsi, L., Ueda, N., Yoshida, S., Fukuda, M., Fukuyasu, Y., Yamauchi, Y., et al. (2005) · Journal of the American Chemical Society · doi:10.1021/ja047018k · Cited by 196
Dirnagl, U., Lindauer, U., Them, A., Schreiber, S., Pfister, H., Koedel, U., Reszka, R., Freyer, D., & Villringer, A. (1995) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.1995.118 · Cited by 159
Tyagi, S. R., TAMURA, M. N., Burnham, D. N., & Lambeth, J. D. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)37690-7 · Cited by 100
Xu, K., Tang, B., Huang, H., Yang, G., Chen, Z., Li, P., & An, L. (2005) · Chemical Communications · doi:10.1039/b512440a · Cited by 100
Self-citationSr2+ activates cross-bridge phosphorylation and latch state in smooth muscle
Hai, C.-M., & Murphy, R. A. (1988) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1988.255.3.c401 · Cited by 59
Effects of Superoxide on Signaling Pathways in Smooth Muscle Cells From Rats
Wu, L., & de Champlain, J. (1999) · Hypertension · doi:10.1161/01.hyp.34.6.1247 · Cited by 45
NILSSON, U. A., Haraldsson, G., BRATELL, S., Sørensen, V., Åkerlund, S., Pettersson, S., Scherstén, T., & Jonsson, O. (1993) · Acta Physiologica Scandinavica · doi:10.1111/j.1748-1716.1993.tb09498.x · Cited by 37
Blass, J. P., Cyrus, P. A., Bieber, F., & Gulanski, B. (2000) · Alzheimer Disease & Associated Disorders · doi:10.1097/00002093-200001000-00005 · Cited by 30
Beni, S. M., Tsenter, J., Alexandrovich, A. G., Galron-Krool, N., Barzilai, A., Kohen, R., Grigoriadis, N., Simeonidou, C., & Shohami, E. (2005) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/sj.jcbfm.9600209 · Cited by 22
Махаева, Г. Ф., Fomicheva, S. B., Zhuravleva, L. V., Yarkevich, A. N., Kharitonov, A. V., & Malygin, V. V. (2001) · Pharmaceutical Chemistry Journal · doi:10.1023/a:1010471710580 · Cited by 9
Research works printed but not linked: 4
- Afanas′ev I. B., 2005, Med. Hypotheses, 64
- Mashkovskii M. D., Drugs (in Russian), Vol. 1, 201
- Fang Y. Z., 2002, Theory and application of free radical biology, 191
- Sodhi A., 1992, Clin. Exp. Immunol., 888, 350
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Yang, Y., Zhao, Q., Feng, W., & Li, F. (2012) · Chemical Reviews · doi:10.1021/cr2004103 · Cited by 2,244
Self-citationFluorescent Chemosensors Based on Spiroring-Opening of Xanthenes and Related Derivatives
Chen, X., Pradhan, T., Wang, F., Kim, J. S., & Yoon, J. (2011) · Chemical Reviews · doi:10.1021/cr200201z · Cited by 1,997
Design Strategies for Water-Soluble Small Molecular Chromogenic and Fluorogenic Probes
Li, X., Gao, X., Shi, W. S., & Ma, H. (2013) · Chemical Reviews · doi:10.1021/cr300508p · Cited by 1,745
Fluorescent and luminescent probes for detection of reactive oxygen and nitrogen species
Chen, X., Tian, X., Shin, I., & Yoon, J. (2011) · Chemical Society Reviews · doi:10.1039/c1cs15037e · Cited by 980
Du, J., Hu, M., Fan, J., & Peng, X. (2012) · Chemical Society Reviews · doi:10.1039/c2cs00004k · Cited by 691
Metadata
- Authors
- Xu, K., Liu, X., Tang, B., Yang, G., Yang, Y., & An, L.
- Affiliations
- Shandong Normal University (CN)
- Shandong University of Traditional Chinese Medicine (CN)
- Corresponding author
- Xu, Kehua
- Liu, Xia
- Tang, Bo
- Yang, Guiwen
- Yang, Yong
- An, Liguo
- Venue
- Chemistry – A European Journal
- Publisher
- Wiley
- Published
- 2007-01-18
- Volume
- 13
- Issue
- 5
- Pages
- 1411-1416
- ISSN
- 0947-6539 (ISSN-L)
- 1521-3765
- OpenAlex
- W2172088091
- Cited by
- 105
- References
- 21
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 7 · 2023: 7 · 2022: 2 · 2021: 10 · 2020: 4 · 2019: 10 · 2018: 10 · 2017: 4 · 2016: 6 · 2015: 7 · 2014: 1 · 2013: 13 · 2012: 9
- FWCI
- 0.79
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Chemistry - A European Journal (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
- Keywords
- superoxide detection, fluorescent probes, reactive oxygen species, cell-permeable probes, mouse peritoneal macrophages, confocal microscopy imaging, oxidative stress
- MeSH terms
- Animals, Cell Line, Fluoresceins, Fluorescent Dyes, Homeostasis, Oxygen, Phosphinic Acids, Sensitivity and Specificity, Spectrometry, Fluorescence, Superoxides, Time Factors, Reactive Oxygen Species, Macrophages, Peritoneal, Mice
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