Development of a Three‐Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling
Open accessAbstract
This unit describes a protocol for generation of lung organoids. A lung organoid is a 3D cell/hydrogel composite that resembles the morphology and cellular composition of the human distal lung. These tissue‐engineered constructs provide an in vitro model of human lung and are best suited for disease modeling applications. The organoid generation methodology is flexible, allowing for easy scalability in the number of organoids produced and in the ability to accommodate a wide range of cell types. © 2018 by John Wiley & Sons, Inc.
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Organoid types grown in alginate include human lung, human brain, murine intestinal, human intestinal, human pancreatic, and human and murine vascular.
“Organoid types grown in alginate include human lung99,100, human brain101, murine intestinal102, human intestinal102,103, human pancreatic104, and human and murine vascular105.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [100]
Several laboratories have grown a wide range of organoids in polysaccharides such as alginate or alginate-chitosan mixtures.
“In addition to organoids grown in naturally-derived proteins, several laboratories have grown a wide range of organoids in polysaccharides such as alginate or alginate-chitosan mixtures.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [100]
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Self-citation
- Authors
- Wilkinson, D. C., Mellody, M., Meneses, L. K., Hope, A. C., Dunn, B., & Gomperts, B. N.
- Affiliations
- University of California, Los Angeles (US)
- Corresponding author
- Gomperts, Brigitte N.
- Venue
- Current Protocols in Stem Cell Biology
- Publisher
- Wiley
- Published
- 2018-06-07
- Volume
- 46
- Issue
- 1
- Pages
- e56-e56
- ISSN
- 1938-8969 (ISSN-L)
- 1941-7322
- PMC
- PMC6105393
- OpenAlex
- W2805992230
- Cited by
- 25
- References
- 12
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 2 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 2 · 2018: 1
- FWCI
- 0.90
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Current Protocols in Stem Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- National Science Foundation
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Neonatal Respiratory Health Research
- Keywords
- lung organoids, 3D cell culture, hydrogel scaffolds, distal lung, tissue engineering, in vitro lung model
- MeSH terms
- Alginates, Animals, Fibroblasts, Lung, Microspheres, Organoids, Bioreactors, Imaging, Three-Dimensional, Collagen Type I, Rats, Static Electricity, Bioengineering, Precision Medicine
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