The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Autofluorescence—an important ancillary technique for the detection of Mycobacterium tuberculosis: Revisited
Abstract
Tuberculosis is one of the oldest diseases known to affect humans and is caused by Mycobacterium tuberculosis. Lymph nodes are the most common site of extrapulmonary involvement. Tuberculous lymphadenitis can be presumptively diagnosed morphologically on fine‐needle aspiration biopsy of lymph nodes. Additional tests like Ziehl‐Neelsen (ZN) stain for detection of acid fast bacilli, fluorescence, and molecular markers are often employed for confirmation of diagnosis. However, culture is essential for obtaining a definitive diagnosis. Unfortunately, culture is time consuming and expensive. Newer investigative methods are required. The efficacy of autofluorescence in the diagnosis of tuberculous lymphadenitis was evaluated for this purpose. Fine‐needle aspiration biopsies were collected from patients with a clinical diagnosis of tuberculous lymphadenitis. Cytomorphological examination, ZN staining, autofluorescence, and culture were performed on all specimens. ZN staining was positive in 37.5% (30/80) of cases, while autofluorescence was positive in 57.5% (46/80) of patients and was found to have 81.8% specificity, 95% sensitivity, and a positive predictive value of 82.6%. This technique allowed rapid and early diagnosis of tuberculous lymphadenitis in a number of patients, permitting early institution of appropriate therapy. Diagn. Cytopathol. 2013;41:330–334. © 2012 Wiley Periodicals, Inc.
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In other studies on FNA smears of lymph nodes, autofluorescence was found to be more sensitive than ZN staining, but compared to cytodiagnosis, it was less sensitive.
“In other studies on FNA smears of lymph nodes, autofluorescence was found to be more sensitive than ZN staining [11, 12], but as compared to cytodiagnosis, it was less sensitive [9].”
From Correlation of Various Techniques in Diagnosis of Tuberculous Lymphadenitis on Fine Needle Aspiration Cytology (2013) · cites this paper as [12]
Citations
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Mycobacterial autofluorescence in Papanicolaou‐stained lymph node aspirates: A glimmer in the dark?
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FINE NEEDLE ASPIRATION CYTOLOGY FOR THE DIAGNOSIS OF TUBERCULOUS LYMPHADENITIS
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- WHO, World Health Organization: Global tuberculosis control, surveillance, planning, financing
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Esteban, J., & García-Coca, M. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.02651 · Cited by 236
“Confocal laser scanning microscopy (CLSM) combined with two fluorescent dyes, Nile Red© (Sigma-Aldrich Co., St. Louis, MO, USA) and LIVE/DEAD BacLight© (Invitrogen, USA), has been used to analyze the phenotypic characteristics of biofilms formed by some RGM (growth rate, percentage of covered surface, percentage of li…”
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Self-citationImportance of Cytopathologic Diagnosis in Early Cancer Diagnosis in Resource-Constrained Countries
Yadav, K., Cree, I., Field, A., Vielh, P., & Mehrotra, R. (2022) · JCO Global Oncology · doi:10.1200/go.21.00337 · Cited by 37
“This may help avoid lengthy delays and costs.(74,75)”
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“Autofluorescence has been found in different mycobacterial species (16, 19).”
BackgroundTuberculosis revisited: Cytological perspective
Chatterjee, D., & Dey, P. (2014) · Diagnostic Cytopathology · doi:10.1002/dc.23190 · Cited by 16
“This method markedly increases the sensitivity of detection of mycobacterial over conventional ZN stain, with sensitivity as high as 95%.(67) This can detect bacilli when present in low concentration (<10(6) bacilli/mL) in clinical material like sputum.”
MethodDetection of Autofluorescent Mycobacterium Chelonae in Living Zebrafish
Whipps, C. M., Moss, L. G., Sisk, D. M., Murray, K. N., Tobin, D. M., & Moss, J. B. (2014) · Zebrafish · doi:10.1089/zeb.2012.0863 · Cited by 12
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New directions using reporter enzyme fluorescence (REF) as a tuberculosis diagnostic platform
Sule, P., Tilvawala, R. R., Behinaein, P., Walkup, G. K., & Cirillo, J. D. (2016) · Tuberculosis · doi:10.1016/j.tube.2016.09.009 · Cited by 11
“Although autofluorescence has been utilized with some success to diagnose tuberculosis [5,16], the thresholds of detection are thought to be much higher than the 10 CFU needed for REF, requiring more than 106 bacteria/ml sputum [16].”
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Diagnosis of Infectious Diseases by Oral Cytology
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Jayakumar, P., Kaushal, M., & Duggal, N. (2023) · Indian Journal of Pathology and Oncology · doi:10.18231/j.ijpo.2023.055
Metadata
- Authors
- Joshi, P., Singh, M., Bhargava, A., Singh, M., & Mehrotra, R.
- Affiliations
- Motilal Nehru Medical College (IN)
- Corresponding author
- Mehrotra, Ravi
- Venue
- Diagnostic Cytopathology
- Publisher
- Wiley
- Published
- 2012-02-20
- Volume
- 41
- Issue
- 4
- Pages
- 330-334
- ISSN
- 1097-0339 (ISSN-L)
- 8755-1039
- OpenAlex
- W2078401180
- Cited by
- 13
- References
- 12
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 1 · 2018: 1 · 2016: 3 · 2014: 3 · 2013: 1 · 2012: 2
- FWCI
- 1.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Diagnostic Cytopathology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Infectious Diseases › Diagnosis and treatment of tuberculosis
- Health Sciences › Medicine › Pathology and Forensic Medicine › Lymphadenopathy Diagnosis and Analysis
- Keywords
- autofluorescence, Mycobacterium tuberculosis, tuberculous lymphadenitis, fine-needle aspiration, Ziehl-Neelsen staining, acid-fast bacilli, sensitivity and specificity, extrapulmonary tuberculosis, rapid diagnosis
- MeSH terms
- Adolescent, Adult, Child, Child, Preschool, Culture Media, Female, Humans, Male, Middle Aged, Mycobacterium tuberculosis, Sensitivity and Specificity, Staining and Labeling, Tuberculosis, Lymph Node, Biopsy, Fine-Needle, Young Adult, Optical Imaging
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