The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
MicroCT for developmental biology: A versatile tool for high‐contrast 3D imaging at histological resolutions
Open accessAbstract
Understanding developmental processes requires accurate visualization and parameterization of three‐dimensional embryos. Tomographic imaging methods offer automatically aligned and calibrated volumetric images, but the usefulness of X‐ray CT imaging for developmental biology has been limited by the low inherent contrast of embryonic tissues. Here, I demonstrate simple staining methods that allow high‐contrast imaging of embryonic tissues at histological resolutions using a commercial microCT system. Quantitative comparisons of images of chick embryos treated with different contrast agents show that three very simple methods using inorganic iodine and phosphotungstic acid produce overall contrast and differential tissue contrast for X‐ray imaging at least as high as that obtained with osmium. The stains can be used after any common fixation and after storage in aqueous or alcoholic media, and on a wide variety of species. These methods establish microCT imaging as a useful tool for comparative developmental studies, embryo phenotyping, and quantitative modeling of development. Developmental Dynamics 238:632–640, 2009. © 2009 Wiley‐Liss, Inc.
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Cites
A series of normal stages in the development of the chick embryo
Hamburger, V., & Hamilton, H. L. (1992) · Developmental Dynamics · doi:10.1002/aja.1001950404 · Cited by 2,207
Histological and Histochemical Methods: Theory and Practice
Kiernan, J. A. (1981) · Cited by 1,359
Principles and Techniques of Electron Microscopy: Biological Applications
Maugel, T. K., & Hayat, M. A. (1979) · Transactions of the American Microscopical Society · doi:10.2307/3226038 · Cited by 1,316
Histologic fixatives suitable for diagnostic light and electron microscopy.
McDowell, E. M., & Trump, B. F. (1976) · PubMed · Cited by 1,254
Histological and Histochemical Methods - Theory and practice
Malatesta, M. (2016) · European Journal of Histochemistry · doi:10.4081/ejh.2016.2639 · Cited by 1,168
Computed tomography : fundamentals, system technology, image quality, applications
Kalender, W. A. (2000) · Cited by 698
Humason's Animal tissue techniques
Presnell, J. K., Schreibman, M. P., & Humason, G. L. (1997) · Johns Hopkins University Press eBooks · Cited by 458
Larabell, C. A., & Le Gros, M. A. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e03-07-0522 · Cited by 282
BETZ, O., WEGST, U., WEIDE, D., HEETHOFF, M., HELFEN, L., LEE, W., & CLOETENS, P. (2007) · Journal of Microscopy · doi:10.1111/j.1365-2818.2007.01785.x · Cited by 277
Principles and Techniques of Electron Microscopy: Biological Applications
Goode, M. D., & Hayat, M. A. (1973) · Transactions of the American Microscopical Society · doi:10.2307/3224936 · Cited by 253
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Self-citationGignac, P. M., Kley, N. J., Clarke, J. A., Colbert, M. W., Morhardt, A. C., Cerio, D., Cost, I. N., Cox, P. G., Daza, J. D., Early, C. M., et al. (2016) · Journal of Anatomy · doi:10.1111/joa.12449 · Cited by 541
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Metadata
- Authors
- Metscher, B. D.
- Venue
- Developmental Dynamics
- Publisher
- Wiley
- Published
- 2009-02-22
- Volume
- 238
- Issue
- 3
- Pages
- 632-640
- ISSN
- 1058-8388 (ISSN-L)
- 1097-0177
- OpenAlex
- W2142002310
- Cited by
- 607
- References
- 23
- FWCI
- 17.89
- Type
- journal article
- Language
- en
- Open access
- bronze
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