Generation of Ppp2Ca and Ppp2Cb conditional null alleles in mouse
Abstract
Protein phosphatase 2A (PP2A) is one of the most abundant serine/threonine phosphatases, with a critical role in embryonic development and human disease. There are two isoforms of the catalytic subunit of PP2A, Ppp2ca and Ppp2cb. Null mutation of Ppp2ca leads to early embryonic lethality at E6.5, hindering functional study of PP2A beyond this stage. We generated conditional null alleles of Ppp2ca and Ppp2cb by flanking with loxP sites exons 3 to 5 of Ppp2ca and exon 3 of Ppp2cb. Ppp2ca(fl/fl) mice did not display any visible phenotype. Homozygous mutants in which Cre-mediated excision resulted in global deletion of Ppp2ca displayed embryonic lethality and developmental defects similar to those previously reported. Ppp2cb(Δ/Δ) mice generated by the same strategy did not display any obvious morphological or physiological defects. These mouse strains can serve as important genetic tools to study the roles of PP2A during development and disease in a spatial- or temporal-specific manner.
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Ppp2cb conditional knockout mice showed no obvious embryonic or adult phenotype, behaved normally, and were fertile.
“On the other hand, Ppp2cb conditional KO mice showed no obvious embryonic or adult phenotype, behaved normally and were fertile [35].”
From Physiologic functions of PP2A: Lessons from genetically modified mice (2019) · cites this paper as [35]
Very similar observations were made in a conditional Ppp2ca knockout model: smaller, degenerated embryos at E6.5, no mesoderm formation, and no Brachyury expression.
“Very similar observations were made in a conditional Ppp2ca KO mouse model, in which again smaller, degenerated embryos were observed at E6.5, with no mesoderm formation and no Brachyury expression [35].”
From Physiologic functions of PP2A: Lessons from genetically modified mice (2019) · cites this paper as [35]
Homozygous deletion of PPP2R1A and PPP2CA in mice is embryonically lethal.
“More importantly, knockout (KO) studies in mice have revealed that the homozygous deletion of PPP2R1A and PPP2CA is embryonically lethal [ 36 – 38 ].”
From Targeting PP2A in cancer: an underrated option (2025) · cites this paper as [37]
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- Authors
- Gu, P., Qi, X., Zhou, Y., Wang, Y., & Gao, X.
- Affiliations
- Model Animal Research Center (CN)
- Corresponding author
- Gao, Xiang
- Venue
- genesis
- Publisher
- Wiley
- Published
- 2011-12-27
- Volume
- 50
- Issue
- 5
- Pages
- 429-436
- ISSN
- 1526-954X (ISSN-L)
- 1526-968X
- OpenAlex
- W2003424246
- Cited by
- 51
- References
- 18
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 3 · 2023: 1 · 2022: 9 · 2021: 4 · 2020: 4 · 2019: 4 · 2018: 2 · 2017: 3 · 2016: 5 · 2015: 3 · 2014: 1 · 2013: 3 · 2012: 2
- FWCI
- 0.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- genesis (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- protein phosphatase 2A, PPP2CA, embryonic lethality, developmental defects, mouse model
- MeSH terms
- Alleles, Animals, Base Sequence, Isoenzymes, Blotting, Southern, Blotting, Western, DNA Primers, Mice, Knockout, Reverse Transcriptase Polymerase Chain Reaction, Mice, Protein Phosphatase 2, Real-Time Polymerase Chain Reaction
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