Oxidative stress delays development and alters gene expression in the agricultural pest moth, Helicoverpa armigera
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Abstract
Abstract Stress is a widespread phenomenon that all organisms must endure. Common in nature is oxidative stress, which can interrupt cell homeostasis to cause cell damage and may be derived from respiration or from environmental exposure thought diet. As a result of the routine exposure from respiration, many organisms can mitigate the effects of oxidative stress, but less is known about responses to oxidative stress from other sources. Helicoverpa armigera is a major agricultural pest moth that causes significant damage to crops worldwide. Here, we examined the effects of oxidative stress on H. armigera by chronically exposing individuals to paraquat - a free radical producer - and measuring changes in development (weight, developmental rate, lifespan), and gene expression. We found that oxidative stress strongly affected development in H. armigera , with stressed samples spending more time as caterpillars than control samples (>24 vs. ∼15 days, respectively) and living longer overall. We found 1,618 up- and 761 down-regulated genes, respectively, in stressed vs. control samples. In the up-regulated gene set were genes associated with cell senescence and apoptosis and an over-representation of biological processes related to cuticle and chitin development, glycine metabolism, and oxidation-reduction. Oxidative stress clearly impacts physiology and biochemistry in H. armigera and the interesting finding of an extended lifespan in stressed individuals could demonstrate hormesis, the process whereby toxic compounds can actually be beneficial at low doses. Collectively, our findings provide new insights into genomic responses to oxidative stress in invertebrates.
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Larvae under oxidative stress take significantly longer time for pupation compared to normal larvae.
“A recent study on Cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae) showed that larvae under oxidative stress takes significantly longer time for pupation compared to normal larvae similar to what we observed on larvae feeding on a PAW- diet105.”
From Atmospheric cold plasma alters plant traits and negatively affects the growth and development of fall armyworm in rice (2025) · cites this paper as [105]
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Singh, N., Maheshwari, N., & Qamar, A. (2024) · The Journal of Basic and Applied Zoology · doi:10.1186/s41936-024-00419-0 · Cited by 2 · see citing works
López, C., Muñoz, P., Zanga, D., Girón‐Calva, P. S., & Eizaguirre, M. (2021) · Insects · doi:10.3390/insects12080718 · Cited by 2 · see citing works
Bachler, A., Walsh, T. K., Andrews, D., Williams, M., Tay, W. T., Gordon, K. H. J., James, B., Fang, C., Wang, L., Wu, Y., et al. (2026) · BMC Genomics · doi:10.1186/s12864-026-13139-2 · Cited by 1 · see citing works
Self-citationFor Better or Worse: How Early Life Oxidative Stress Moulds the Phenotype
Costantini, D. (2024) · Fascinating life sciences · doi:10.1007/978-3-031-65183-0_7 · Cited by 1 · see citing works
Apirajkamol, N., Walsh, T., & McGaughran, A. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.12.15.422848 · Cited by 1 · see citing works
Self-citationVirulence of Beauveria sp. and Metarhizium sp. fungi towards Fall armyworm (Spodoptera frugiperda)Preprint
Apirajkamol, N., Hogarty, T. M., Mainali, B. P., Taylor, P. W., Walsh, T. K., & Tay, W. T. (2022) · Research Square · doi:10.21203/rs.3.rs-1833011/v1
Self-citationVirulence of Beauveria sp. and Metarhizium sp. fungi towards Fall armyworm (Spodoptera frugiperda)Preprint
Apirajkamol, N., Hogarty, T. M., Mainali, B. P., Taylor, P. W., Walsh, T. K., & Tay, W. T. (2022) · Research Square · doi:10.21203/rs.3.rs-1833011/v2
Self-citationTunçyürek, T., Genç, H. Y., & Gulec, N. (2026) · Biology Bulletin · doi:10.1134/s1062359025612935
Effects of novaluron exposure on gene expressions in midgut of silkworm, Bombyx mori
Li, M.-L., Zhu, L., Liu, Y.-H., Wang, M.-J., Zhao, G., & Qian, H.-Y. (2026) · Gene Reports · doi:10.1016/j.genrep.2026.102561
Midgut oxidative stress drives salinity-induced growth inhibition of an insect herbivorePreprint
Pawar, S. V., Pawar, M., Mueller, R., Peiffer, M. L., Hermann, S. L., & Felton, G. W. (2026) · SSRN Electronic Journal · doi:10.2139/ssrn.7351687
- Authors
- Apirajkamol, N. (., James, B., Gordon, K. H. J., Walsh, T. K., & McGaughran, A.
- Affiliations
- Australian National University (AU)
- Commonwealth Scientific and Industrial Research Organisation (AU)
- Corresponding author
- McGaughran, Angela
- Venue
- Ecology and Evolution
- Publisher
- Wiley
- Published
- 2020-04-29
- Volume
- 10
- Issue
- 12
- Pages
- 5680-5693
- ISSN
- 2045-7758 (ISSN-L)
- PMC
- PMC7319138
- OpenAlex
- W3021218637
- Cited by
- 26
- References
- 84
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 6 · 2023: 1 · 2022: 3 · 2021: 4 · 2020: 1
- FWCI
- 1.19
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Ecology and Evolution (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- ANU Open Research (Australian National University) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Commonwealth Scientific and Industrial Research Organisation
- Australian Research Council (DE160100685)
- Topics
- Physical Sciences › Environmental Science › Ecology › Physiological and biochemical adaptations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Keywords
- oxidative stress, Helicoverpa armigera, paraquat, gene expression, insect development, lifespan, hormesis, glycine metabolism, redox metabolism, crop pests
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