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Production of hybridoma growth factor by human monocytes
Abstract
Human mononuclear leukocytes produce a growth factor (HGF) for hybridoma and plasmacytoma cells. HGF has recently been proven to be identical to IFN‐β2, 26‐kDa protein and BSF‐2. HGF can be quantitated in a proliferation assay with the HGF‐dependent hybridoma cell line B13.29. By selection of an extremely sensitive variant of this cell line, we were able to measure HGF production of single cells. Limiting dilution analysis of the producing cells in combination with size, density and adherence characteristics showed that HGF is produced by monocytes and not by lymphocytes. There was no need for the monocytes to be stimulated but the cells did require the presence of serum. This serum requirement could be met by purified bovine serum albumin, but not by other proteins like ovalbumin or human γ‐globulin. HGF production in vitro by monocytes starts after 2 h of incubation and is completed within 24 h.
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Citations
Cited by
de Waal Malefyt, R., Abrams, J., Bennett, B., Figdor, C. G., & de Vries, J. E. (1991) · The Journal of experimental medicine · doi:10.1084/jem.174.5.1209 · Cited by 3,762
“This was relatively late as compared to the production of I1,1oe, I1,1/~, I1,6, I1,8 and TNF-o~, which were secreted at high levels between 4-8 h after activation (31-33).”
Sudo, N., Chida, Y., Aiba, Y., Sonoda, J., Oyama, N., Yu, X., Kubo, C., & Koga, Y. (2004) · The Journal of Physiology · doi:10.1113/jphysiol.2004.063388 · Cited by 2,735
Interleukin-6 and the acute phase response
Heinrich, P. C., Castell, J. V., & Andus, T. (1990) · Biochemical Journal · doi:10.1042/bj2650621 · Cited by 2,734
Biological basis of the behavior of sick animals
Hart, B. L. (1988) · Neuroscience & Biobehavioral Reviews · doi:10.1016/s0149-7634(88)80004-6 · Cited by 2,223
Van Snick, J. (1990) · Annual Review of Immunology · doi:10.1146/annurev.iy.08.040190.001345 · Cited by 1,890
Metadata
- Authors
- Aarden, L. A., De Groot, E. R., Schaap, O. L., & Lansdorp, P. M.
- Venue
- European Journal of Immunology
- Publisher
- Wiley
- Published
- 1987-01
- Volume
- 17
- Issue
- 10
- Pages
- 1411-1416
- ISSN
- 0014-2980 (ISSN-L)
- 1521-4141
- OpenAlex
- W2122248986
- Cited by
- 1,092
- References
- 0
- FWCI
- 31.05
- Type
- journal article
- Language
- en
- Open access
- closed
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- Aarden, L. A., De Groot, E. R., Schaap, O. L., & Lansdorp, P. M. (1987). Production of hybridoma growth factor by human monocytes. European Journal of Immunology. https://doi.org/10.1002/eji.1830171004
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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