CD161 is a marker of all human IL‐17‐producing T‐cell subsets and is induced by RORC
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We have previously shown that human Th17 lymphocytes are characterized by the selective expression of IL‐23 receptor (IL‐23R), CCR6, CD161, and the transcription factor retinoic acid‐related orphan receptor C (RORC), and originate from a CD161+CD4+ naïve T‐cell precursor in response to the combined activity of IL‐1β and IL‐23. We show here that not only CD4+TCRαβ+, but also CD8+TCRαβ+, CD4−CD8− TCRαβ+, and CD4−CD8− TCRγδ+ circulating lymphocytes that produce IL‐17 express the distinctive marker CD161 on their surface. In addition, we demonstrate that CD161 expression identifies CD8+ and CD4−CD8− umbilical cord blood T cells that already express RORC and IL‐23R mRNA and that can be induced to differentiate into IL‐17‐producing cells in the presence of IL‐1β and IL‐23. Finally, we provide evidence that umbilical cord blood naïve CD4+CD161− T cells, upon lentivirus‐mediated transduction with RORC2 can acquire the ability to express IL‐23R, IL‐1RI, and CD161, as well as to produce IL‐17. Taken together, these data allow to conclude that T‐cell subsets able to produce IL‐17, as well as precursors of IL‐17‐producing T cells, exhibit surface expression of CD161, and that this feature is at least in part RORC2‐dependent.
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Th17 can be distinguished by expression of killer-cell lectin-like receptor CD161 (KLRB1), while Th22 expresses the skin-homing receptor CCR10.
“However, Th17 can be distinguished by the expression of the killer-cell lectin-like receptor CD161 (KLRB1) [155,156], while Th22 expresses the skin-homing receptor CCR10 [154].”
From Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) (2021) · cites this paper as [156]
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- Authors
- Maggi, L., Santarlasci, V., Capone, M., Peired, A., Frosali, F., Crome, S. Q., Querci, V., Fambrini, M., Liotta, F., Levings, M. K., Maggi, E., Cosmi, L., Romagnani, S., & Annunziato, F.
- Affiliations
- University of Florence (IT)
- University of British Columbia (CA)
- Corresponding author
- Romagnani, Sergio
- ORCID
- Venue
- European Journal of Immunology
- Publisher
- Wiley
- Published
- 2010-07-29
- Volume
- 40
- Issue
- 8
- Pages
- 2174-2181
- ISSN
- 0014-2980 (ISSN-L)
- 1521-4141
- OpenAlex
- W1985447596
- Cited by
- 362
- References
- 27
- Citations by year
- 2026: 12 · 2025: 12 · 2024: 14 · 2023: 15 · 2022: 20 · 2021: 24 · 2020: 30 · 2019: 27 · 2018: 19 · 2017: 34 · 2016: 23 · 2015: 29 · 2014: 24 · 2013: 23 · 2012: 30
- FWCI
- 10.70
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- European Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Florence Research (University of Florence) (submittedVersion)
- Funders
- Canadian Institutes of Health Research (MOP93793)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › IL-33, ST2, and ILC Pathways
- Keywords
- CD161, IL-17-producing T cells, RORC, RORγt, Th17 cells, IL23R, IL-1β, IL-23, T cell differentiation, CD8+ T cells, γδ T cells, lentiviral transduction
- MeSH terms
- Cell Differentiation, Cells, Cultured, T-Lymphocytes, Helper-Inducer, Humans, Lymphocyte Activation, Biomarkers, CD4 Antigens, T-Lymphocyte Subsets, Receptors, Antigen, T-Cell, gamma-delta, Receptors, Antigen, T-Cell, alpha-beta, CD8 Antigens, Transgenes, Interleukin-17, Interleukin-1beta, Interleukin-23, NK Cell Lectin-Like Receptor Subfamily B, Nuclear Receptor Subfamily 1, Group F, Member 3
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