The cell wall subproteome of Listeria monocytogenes
Abstract
The surface subproteome of Listeria monocytogenes that includes many proteins already known to be involved in virulence and interaction with host cells has been characterized. A new method for the isolation of a defined surface proteome of low complexity has been established based on serial extraction of proteins by different salts at high concentration, and in all 55 proteins were identified by N-terminal sequencing and mass spectrometry. About 16% of these proteins are of unknown function and three proteins have no orthologue in the nonpathogenic L. innocua and might be involved in virulence mechanisms. Remarkably, a relatively high number of proteins with a function in the cytoplasmic compartment was identified in this surface proteome. These proteins had neither predicted or detectable signal peptides nor could any modification be observed except removal of the N-terminal methionine. Enolase (Lmo2455) is one of these proteins. It was shown to be present in the cell wall of the pathogen by immunoelectron microscopy and, along with heat shock factor DnaK (Lmo1473), elongation factor TU (Lmo2653), and glyceraldehyde-3-phosphate dehydrogenase (Lmo2459), it was found to be able to bind human plasminogen in overlay blots and surface plasmon resonance (SPR) experiments. The KD values of these interactions were determined by SPR measurements. The data indicate a possible role of these proteins as receptors for human plasminogen on the bacterial cell surface. The potential role of this recruitment of a host protease for extracellular invasion mechanisms is discussed.
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Extracellular DnaK, enolase, and GAPDH were identified in this study and in E. faecalis JH2-2; these proteins may play an important role in bacterial adhesion.
“Extracellular DnaK, enolase, and GAPDH were identified in this study and in the laboratory strain, E. faecalis JH2-2 (Benachour et al., 2009), these proteins may play an important role in bacterial adhesion, as they have been reported to have a strong binding capacity to human thrombospondin (Schaumburg et al., 2004).”
From Adhesion and Colonization of the Probiotic Lactobacillus plantarum HC-2 in the Intestine of Litopenaeus Vannamei Are Associated With Bacterial Surface Proteins (2022) · cites this paper as Schaumburg et al., 2004
Ribosomal proteins have been identified not only in the cytoplasm but also on the surface of bacteria, and they may play a key role in mediating bacterial adhesion and colonization.
“Recently, ribosomal proteins have been identified not only in the cytoplasm but also on the surface of bacteria, and they may play a key role in mediating bacterial adhesion and colonization; for example, surface-associated ribosomal proteins have been detected in Streptococcus pseudomallei and Bacillus subtilis 168 (Schaumburg et al., 2004; Severin et al., 2007; Tjalsma”
From Adhesion and Colonization of the Probiotic Lactobacillus plantarum HC-2 in the Intestine of Litopenaeus Vannamei Are Associated With Bacterial Surface Proteins (2022) · cites this paper as Schaumburg et al., 2004
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- Authors
- Schaumburg, J., Diekmann, O., Hagendorff, P., Bergmann, S., Rohde, M., Hammerschmidt, S., Jänsch, L., Wehland, J., & Kärst, U.
- Affiliations
- Lund University (SE)
- Biaffin (Germany) (DE)
- Helmholtz Centre for Infection Research (DE)
- Corresponding author
- Kärst, Uwe
- Venue
- PROTEOMICS
- Publisher
- Wiley
- Published
- 2004-10
- Volume
- 4
- Issue
- 10
- Pages
- 2991-3006
- ISSN
- 1615-9853 (ISSN-L)
- 1615-9861
- OpenAlex
- W2003688768
- Cited by
- 199
- References
- 58
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 6 · 2023: 3 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 9 · 2018: 2 · 2017: 7 · 2016: 6 · 2015: 11 · 2014: 22 · 2013: 15 · 2012: 18
- FWCI
- 10.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- PROTEOMICS (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Listeria monocytogenes in Food Safety
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Listeria monocytogenes, cell surface proteome, virulence factors, plasminogen binding, enolase, DnaK, elongation factor Tu, glyceraldehyde-3-phosphate dehydrogenase, cytosolic proteins, host-pathogen interaction, plasminogen receptor, Listeria innocua
- MeSH terms
- Aminopeptidases, Bacterial Proteins, Cell Wall, Cloning, Molecular, Cytoplasm, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Humans, Kinetics, Ligands, Listeria monocytogenes, Methionine, Plasminogen, Proteins, Salts, Mass Spectrometry, Time Factors, Virulence, Blotting, Western, Electrophoresis, Gel, Two-Dimensional, Microscopy, Immunoelectron, Protein Structure, Tertiary, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Computational Biology, Surface Plasmon Resonance, Sequence Analysis, Protein, Proteome
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