The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Improvement of the solubilization of proteins in two‐dimensional electrophoresis with immobilized pH gradients
Open accessAbstract
Membrane and nuclear proteins of poor solubility have been separated by high resolution two‐dimensional (2‐D) gel electrophoresis. Isoelectric focusing with immobilized pH gradients leads to severe quantitative losses of proteins in the resulting 2‐D map, although the resolution is usually high. Protein solubility could be improved by using denaturing solutions containing various detergents and chaotropes. Best results were obtained with a denaturing solution containing urea, thiourea, and detergents (both nonionic and zwitterionic). The usefulness of thiourea‐containing denaturing mixtures is shown for microsomal and nuclear proteins as well as for tubulin, a protein highly prone to aggregation.
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Cites
Measurement of protein using bicinchoninic acid
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., & Klenk, D. C. (1985) · Analytical Biochemistry · doi:10.1016/0003-2697(85)90442-7 · Cited by 18,482
Cited by
Protein Analysis by Shotgun/Bottom-up Proteomics
Zhang, Y., Fonslow, B. R., Shan, B., Baek, M.-C., & Yates, J. R. (2013) · Chemical Reviews · doi:10.1021/cr3003533 · Cited by 1,491
Proteomics: quantitative and physical mapping of cellular proteins
Blackstock, W. P., & Weir, M. P. (1999) · Trends in Biotechnology · doi:10.1016/s0167-7799(98)01245-1 · Cited by 873
Proteomics Analysis of Cellular Response to Oxidative Stress
Rabilloud, T., Heller, M., Gasnier, F., Luche, S., Rey, C., Aebersold, R., Benahmed, M., Louisot, P., & Lunardi, J. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m106585200 · Cited by 393
Self-citationPeltier, J.-B., Friso, G., Kalume, D. E., Roepstorff, P., Nilsson, F., Adamska, I., & van Wijka, K. J. (2000) · The Plant Cell · doi:10.1105/tpc.12.3.319 · Cited by 355
Use of thiourea to increase the solubility of membrane proteins in two‐dimensional electrophoresis
Rabilloud, T. (1998) · ELECTROPHORESIS · doi:10.1002/elps.1150190526 · Cited by 337
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Metadata
- Authors
- Rabilloud, T., Adessi, C., Giraudel, A., & Lunardi, J.
- Venue
- ELECTROPHORESIS
- Publisher
- Wiley
- Published
- 1997-01
- Volume
- 18
- Issue
- 3-4
- Pages
- 307-316
- ISSN
- 0173-0835 (ISSN-L)
- 1522-2683
- OpenAlex
- W2130381045
- Cited by
- 459
- References
- 1
- FWCI
- 20.13
- Type
- journal article
- Language
- en
- Open access
- green
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- Rabilloud, T., Adessi, C., Giraudel, A., & Lunardi, J. (1997). Improvement of the solubilization of proteins in two‐dimensional electrophoresis with immobilized pH gradients. ELECTROPHORESIS. https://doi.org/10.1002/elps.1150180303
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