The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Use of thiourea to increase the solubility of membrane proteins in two‐dimensional electrophoresis
Abstract
The separation of membrane proteins by high‐resolution two‐dimensional electrophoresis was carried out. At high loads, these proteins are prone to precipitation, resulting in poor resolution. It is shown here that the use of thiourea, previously described for focusing in immobilized pH gradients, can be extended to conventional isoelectric focusing. As thiourea inhibits acrylamide polymerization, a modified photopolymerization system must be used. These modifications result in higher solubility of proteins during IEF, thereby increasing the resolution and capacity of the two‐dimensional gels.
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Cites
Two-dimensional gel electrophoresis of membrane proteins
Ames, G. F. L., & Nikaido, K. (1976) · Biochemistry · doi:10.1021/bi00648a026 · Cited by 697
Rabilloud, T., Adessi, C., Giraudel, A., & Lunardi, J. (1997) · ELECTROPHORESIS · doi:10.1002/elps.1150180303 · Cited by 459
Self-citationRabilloud, T., Valette, C., & Lawrence, J. J. (1994) · ELECTROPHORESIS · doi:10.1002/elps.11501501223 · Cited by 366
Self-citationBjellqvist, B., Sanchez, J., Pasquali, C., Ravier, F., Paquet, N., Frutiger, S., Hughes, G. J., & Hochstrasser, D. (1993) · ELECTROPHORESIS · doi:10.1002/elps.11501401212 · Cited by 187
Adessi, C., Miège, C., Albrieux, C., & Rabilloud, T. (1997) · Electrophoresis · doi:10.1002/elps.1150180124 · Cited by 135
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Cited by
Peltier, J.-B., Friso, G., Kalume, D. E., Roepstorff, P., Nilsson, F., Adamska, I., & van Wijka, K. J. (2000) · The Plant Cell · doi:10.1105/tpc.12.3.319 · Cited by 355
Comparison of different electrophoretic separations of hen egg white proteins
Desert, C., Guérin-Dubiard, C., Nau, F., Jan, G., Val, F., & Mallard, J. (2001) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf001423n · Cited by 123
Empirical evaluation of bone extraction protocols
Cleland, T. P., Voegele, K., & Schweitzer, M. H. (2012) · PLoS ONE · doi:10.1371/journal.pone.0031443 · Cited by 60
Mazzoli, R., Pessione, E., Dufour, M., Laroute, V., Giuffrida, M. G., Giunta, C., Cocaign-Bousquet, M., & Loubière, P. (2010) · Amino Acids · doi:10.1007/s00726-010-0507-5 · Cited by 61
Metadata
- Authors
- Rabilloud, T.
- Venue
- ELECTROPHORESIS
- Publisher
- Wiley
- Published
- 1998-05
- Volume
- 19
- Issue
- 5
- Pages
- 758-760
- ISSN
- 0173-0835 (ISSN-L)
- 1522-2683
- OpenAlex
- W2024657158
- Cited by
- 337
- References
- 5
- FWCI
- 9.02
- Type
- journal article
- Language
- en
- Open access
- closed
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- Rabilloud, T. (1998). Use of thiourea to increase the solubility of membrane proteins in two‐dimensional electrophoresis. ELECTROPHORESIS. https://doi.org/10.1002/elps.1150190526
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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