A Seagrass-Based Biorefinery for Generation of Single-Cell Oils for Biofuel and Oleochemical Production.
Open accessAbstract
Abstract 78 million tons of residual seagrass deposits accumulate annually on shorelines worldwide. These represent an untapped feedstock for fermentative single‐cell oil production, targeted at biofuel and oleochemical generation, without affecting the sensitive marine environment or compromising food security. Seven beach‐cast samples of seagrass (related to Z. marina, Z. noltii, S. filiforme, P. australis, P. oceanic, and T. testudinum) were collected from marine ecosystems around the world. A combination of 18S rRNA phylogenetic, structural, and comprehensive biomass analyses of seagrass leaves were applied. The carbohydrate content ranged from 73 to 81 % (w/dwbiomass). Single‐step enzymatic hydrolysis was developed to efficiently release the monomeric sugars contained in seagrasses biomass without any pretreatment. P. oceanica hydrolysate allowed for higher lipid yields (6.8 g L−1) compared to the synthetic minimal medium (5.1 g L−1) in shake flasks, and was subsequently utilized as the sole fermentation medium for oleaginous yeast T. oleaginosus at a technical scale using a fed‐batch bioreactor, which provided 24.5 g L−1 lipids (0.35 g L−1 h−1). Moreover, the sugar/lipid conversion ratio was 0.41 (w/w). Cumulative data indicates that by exploiting only half of the global beach‐cast seagrass, approximately 4 million tons of microbial oils could be generated.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Estimations of up to 40 million tons of dry seagrass biomass, which can be used for biofuel production, are given.
“Estimations of up to 40 million tons of dry seagrass biomass, which can be used for biofuel production, are given.”
From The potential of biofuels from first to fourth generation (2023) · cites this paper as [61]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189 · see citing works
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/y59-099 · Cited by 23,189 · see citing works
Analysis, synthesis, and design of chemical processes
(1998) · Choice Reviews Online · doi:10.5860/choice.36-0974 · Cited by 3,433 · see citing works
Seagrass ecosystems as a globally significant carbon stock
Fourqurean, J. W., Duarte, C. M., Kennedy, H. A., Marbà, N., Holmer, M., Mateo, M. Á., Apostolaki, E. T., Kendrick, G. A., Krause‐Jensen, D., McGlathery, K. J., et al. (2012) · Nature Geoscience · doi:10.1038/ngeo1477 · Cited by 2,107 · see citing works
Bioconversion of lignocellulose: inhibitors and detoxification
Jönsson, L. J., Alriksson, B., & Nilvebrant, N. (2013) · Biotechnology for Biofuels · doi:10.1186/1754-6834-6-16 · Cited by 1,385 · see citing works
Kurtzman, C. P., & Robnett, C. J. (1997) · Journal of Clinical Microbiology · doi:10.1128/jcm.35.5.1216-1223.1997 · Cited by 963 · see citing works
Designer synthetic media for studying microbial-catalyzed biofuel production
Tang, X., Sousa, L. D. C., Jin, M., Chundawat, S. P. S., Chambliss, C. K., Lau, M. W., Xiao, Z., Dale, B. E., & Balan, V. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-014-0179-6 · Cited by 889 · see citing works
Enríquez, S., Duarte, C., & Sand-Jensen, K. (1993) · Oecologia · doi:10.1007/bf00566960 · Cited by 883 · see citing works
Green, E. P., & Short, F. T. (2004) · Choice Reviews Online · doi:10.5860/choice.41-3160 · Cited by 777 · see citing works
High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture
Li, Y., Zhao, Z. K., & Bai, F. (2007) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2007.02.008 · Cited by 698 · see citing works
Lipids of oleaginous yeasts. Part I: Biochemistry of single cell oil production
Papanikolaou, S., & Aggelis, G. (2011) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201100014 · Cited by 683 · see citing works
Oily yeasts as oleaginous cell factories
Ageitos, J. M., Vallejo, J. A., Veiga‐Crespo, P., & Villa, T. G. (2011) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3200-z · Cited by 578 · see citing works
Dı́ez, B., Pedrós‐Alió, C., & Massana, R. (2001) · Applied and Environmental Microbiology · doi:10.1128/aem.67.7.2932-2941.2001 · Cited by 505 · see citing works
Phylogenetic Studies in Alismatidae, II: Evolution of Marine Angiosperms (Seagrasses) and Hydrophily
Les, D. H., Cleland, M. A., & Waycott, M. (1997) · Systematic Botany · doi:10.2307/2419820 · Cited by 441 · see citing works
Current Pretreatment Technologies for the Development of Cellulosic Ethanol and Biorefineries
Silveira, M. H. L., Morais, A. R. C., Lopes, A. M. D. C., Olekszyszen, D. N., Bogel‐Łukasik, R., Andreaus, J., & Ramos, L. P. (2015) · ChemSusChem · doi:10.1002/cssc.201500282 · Cited by 385 · see citing works
Koutinas, A., Chatzifragkou, A., Kopsahelis, N., Papanikolaou, S., & Kookos, I. K. (2013) · Fuel · doi:10.1016/j.fuel.2013.08.045 · Cited by 372 · see citing works
Meesters, P. A. E. P., Huijberts, G. N. M., & Eggink, G. (1996) · Applied Microbiology and Biotechnology · doi:10.1007/s002530050731 · Cited by 366 · see citing works
Using marine macroalgae for carbon sequestration: a critical appraisal
Chung, I. K., Beardall, J., Mehta, S., Sahoo, D., & Stojkovic, S. (2010) · Journal of Applied Phycology · doi:10.1007/s10811-010-9604-9 · Cited by 364 · see citing works
Catalytic deoxygenation of microalgae oil to green hydrocarbons
Zhao, C., Brück, T., & Lercher, J. A. (2013) · Green Chemistry · doi:10.1039/c3gc40558c · Cited by 330 · see citing works
Self-citationNitsos, C. K., Matis, K. A., & Triantafyllidis, K. S. (2012) · ChemSusChem · doi:10.1002/cssc.201200546 · Cited by 327 · see citing works
Phosphate-limitation mediated lipid production by Rhodosporidium toruloides
Wu, S., Hu, C., Jin, G., Zhao, X., & Zhao, Z. K. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.02.111 · Cited by 280 · see citing works
Griffiths, M. J., van Hille, R. P., & Harrison, S. T. L. (2010) · Lipids · doi:10.1007/s11745-010-3468-2 · Cited by 277 · see citing works
The ecology of algal biodiesel production
Smith, V. H., Sturm, B. S. M., deNoyelles, F. J., & Billings, S. A. (2010) · Trends in Ecology & Evolution · doi:10.1016/j.tree.2009.11.007 · Cited by 277 · see citing works
Guldhe, A., Singh, B., Rawat, I., Ramluckan, K., & Bux, F. (2014) · Fuel · doi:10.1016/j.fuel.2014.02.059 · Cited by 239 · see citing works
Rakicka, M., Lazar, Z., Dulermo, T., Fickers, P., & Nicaud, J. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0286-z · Cited by 218 · see citing works
Photosynthesis of marine macroalgae and seagrasses in globally changing CO2 environments
Beer, S., & Koch, E. (1996) · Marine Ecology Progress Series · doi:10.3354/meps141199 · Cited by 198 · see citing works
Separation and quantification of microalgal carbohydrates
Templeton, D. W., Quinn, M., Wychen, S. V., Hyman, D., & Laurens, L. M. L. (2012) · Journal of Chromatography A · doi:10.1016/j.chroma.2012.10.034 · Cited by 188 · see citing works
An integrated renewable energy park approach for algal biofuel production in United States
Subhadra, B. G., & Edwards, M. (2010) · Energy Policy · doi:10.1016/j.enpol.2010.04.036 · Cited by 161 · see citing works
Optimization of Culture Conditions for Lipid Production by Rhodosporidium toruloides
Li, Y., Liu, B., Zhao, Z. K., et al. (2006) · Chinese journal of biotechnology/Shengwu gongcheng xuebao · doi:10.1016/s1872-2075(06)60050-2 · Cited by 150 · see citing works
Oil Production fromYarrowia lipolyticaPo1g Using Rice Bran Hydrolysate
Tsigie, Y. A., Wang, C., Kasim, N. S., Do, Q.-D., Huynh, L., Ho, Q. P., Truong, C.-T., & Ju, Y. (2012) · BioMed Research International · doi:10.1155/2012/378384 · Cited by 103 · see citing works
Structure and properties of fibres from sea-grass (Zostera marina)
Davies, P., Morvan, C., Sire, O., & Baley, C. (2007) · Journal of Materials Science · doi:10.1007/s10853-006-0546-1 · Cited by 96 · see citing works
Huang, H., Guo, X., Li, D., Liu, M., Wu, J., & Ren, H. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.05.008 · Cited by 89 · see citing works
Falco, G. D., Simeone, S., & Baroli, M. (2008) · Journal of Coastal Research · doi:10.2112/06-0800.1 · Cited by 87 · see citing works
Lipid production from sweet sorghum bagasse through yeast fermentation
Liang, Y., Tang, T., Siddaramu, T., et al. (2011) · Renewable Energy · doi:10.1016/j.renene.2011.09.035 · Cited by 87 · see citing works
[Fusion tags technology and their applications].
Li, Y.-J., Chen, Y., & Bi, L.-J. (2006) · PubMed · Cited by 80 · see citing works
Zha, Y., Muilwijk, B., & Coulier, L. (2012) · Journal of Bioprocessing & Biotechniques · doi:10.4172/2155-9821.1000112 · Cited by 79 · see citing works
Wei, Y., Siewers, V., & NIELSEN, J. K. (2017) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-017-8126-7 · Cited by 79 · see citing works
Kourist, R., Bracharz, F., Lorenzen, J., Kracht, O. N., Chovatia, M., Daum, C. G., Deshpande, S., Lipzen, A., Nolan, M., Ohm, R. A., et al. (2015) · mBio · doi:10.1128/mbio.00918-15 · Cited by 74 · see citing works
Self-citationSubhash, G. V., & Mohan, S. V. (2013) · Fuel · doi:10.1016/j.fuel.2013.08.035 · Cited by 70 · see citing works
Lipid production from corn stover by the oleaginous yeast Cryptococcus curvatus
Gong, Z., Shen, H., Yang, X., Wang, Q., Xie, H., & Zhao, Z. K. (2014) · Biotechnology for Biofuels · doi:10.1186/s13068-014-0158-y · Cited by 70 · see citing works
Chang, Y.-H., Chang, K., Hsu, C., et al. (2012) · Fuel · doi:10.1016/j.fuel.2012.10.033 · Cited by 64 · see citing works
Kato, Y., Aioi, K., Omori, Y., et al. (2003) · Genes & Genetic Systems · doi:10.1266/ggs.78.329 · Cited by 62 · see citing works
Optimization of the process of chemical hydrolysis of cellulose to glucose
de la Rosa, S. M., Campos‐Martín, J. M., & Fierro, J. L. G. (2014) · Cellulose · doi:10.1007/s10570-014-0280-9 · Cited by 61 · see citing works
Temperature shifts in regulation of lipids accumulated byLipomyces starkeyi
Suutari, M. H., Priha, P., & Laakso, S. (1993) · Journal of the American Oil Chemists Society · doi:10.1007/bf02545349 · Cited by 54 · see citing works
Wang, J., Pan, X., Han, Y. C., et al. (2012) · Marine Drugs · doi:10.3390/md10122729 · Cited by 52 · see citing works
Gujjari, P., Suh, S., Coumes, K., & Zhou, J. J. (2011) · Mycologia · doi:10.3852/10-403 · Cited by 48 · see citing works
Oil Production by the Oleaginous Yeast Lipomyces starkeyi using Diverse Carbon Sources
Wang, R., Wang, J., Xu, R., Fang, Z. Y., & Liu, A. (2014) · BioResources · doi:10.15376/biores.9.4.7027-7040 · Cited by 46 · see citing works
Cebrián, J., Duarte, C. M., & Marbà, N. (1996) · Journal of Experimental Marine Biology and Ecology · doi:10.1016/0022-0981(96)02574-9 · Cited by 43 · see citing works
Zhang, G., French, W. T., Hernandez, R. A., Hall, J., Sparks, D., & Holmes, W. E. (2011) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.2592 · Cited by 43 · see citing works
Asif, S., Chuah, L. F., Klemeš, J. J., Ahmad, M., Akbar, M. M., Lee, K. T., & Fatima, A. (2017) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2017.02.081 · Cited by 38 · see citing works
Transformation of Posidonia oceanica residues to bioethanol
Pilavtepe, M., Çeliktaş, M. S., Sargın, S., & Yeşil-Çeliktaş, Ö. (2013) · Industrial Crops and Products · doi:10.1016/j.indcrop.2013.09.020 · Cited by 37 · see citing works
Lipid production with Trichosporon oleaginosus in a membrane bioreactor using microalgae hydrolysate
Meo, A., Priebe, X., & Weuster‐Botz, D. (2016) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2016.10.021 · Cited by 37 · see citing works
Zhang, X., Yan, S., Tyagi, R. D., et al. (2014) · Fuel · doi:10.1016/j.fuel.2014.05.089 · Cited by 33 · see citing works
Production of 16.5% v/v ethanol from seagrass seeds
Uchida, M., Miyoshi, T., Kaneniwa, M., Ishihara, K., Nakashimada, Y., & Urano, N. (2014) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2014.05.017 · Cited by 30 · see citing works
Quality of the Seagrass Halophila ovalis on a Thai Intertidal Flat as Food for the Dugong
Yamamuro, M., & Chirapart, A. (2005) · Journal of Oceanography · doi:10.1007/s10872-005-0030-6 · Cited by 30 · see citing works
SYNTHESIS OF LIPIDS BY CERTAIN YEAST STRAINS GROWN ON WHEY PERMEATE
AKHTAR, P., Gray, J., & Asghar, A. (1998) · Journal of Food Lipids · doi:10.1111/j.1745-4522.1998.tb00125.x · Cited by 30 · see citing works
Siegal-Willott, J. L., Harr, K., Hayek, L.-A. C., Scott, K. C., Gerlach, T., Sirois, P., Reuter, M., Crewz, D. W., & Hill, R. C. (2010) · Journal of Zoo and Wildlife Medicine · doi:10.1638/2009-0118.1 · Cited by 28 · see citing works
Two-step process for production of biodiesel blends from oleaginous yeast and microalgae
McCurdy, A. T., Higham, A. J., Morgan, M. R., Quinn, J. C., & Seefeldt, L. C. (2014) · Fuel · doi:10.1016/j.fuel.2014.07.099 · Cited by 25 · see citing works
Tsigie, Y. A., Huynh, L. H., Nguyen, P. L. T., et al. (2013) · Fuel Processing Technology · doi:10.1016/j.fuproc.2013.03.036 · Cited by 12 · see citing works
Production of Fischer–Tropsch hydrocarbons via oxygen-blown gasification of charred pinewood pellets
Muradov, N., Gujar, A. C., Baik, J. H., et al. (2015) · Fuel Processing Technology · doi:10.1016/j.fuproc.2015.09.009 · Cited by 11 · see citing works
Liu, Y., Jiang, G., & Wu, Z. (2010) · Journal of Ocean University of China · doi:10.1007/s11802-010-0068-y · Cited by 9 · see citing works
A Search for a Single DNA Barcode for Seagrasses of the World
Daru, B. H., & Yessoufou, K. (2016) · Springer eBooks · doi:10.1007/978-3-319-41840-7_19 · Cited by 8 · see citing works
Brudecki, G. P., Farzanah, R., Cybulska, I., et al. (2015) · Energy Procedia · doi:10.1016/j.egypro.2015.07.508 · Cited by 6 · see citing works
Alipour, S., Omidvarborna, H., Relue, P., et al. (2017) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2017.05.130 · Cited by 1 · see citing works
Flagged first, then by citations
Research works printed but not linked: 14
- G. V. Subhash,S .V .Mohan, Fuel 2014, 116,5 09 -515.
- M. Rakicka,Z .L azar, T. Dulermo, P. Fickers,J .M.N icaud, Bio- technol. Biofuels 2015, 8,1 ;
- R.W ang, J. Wang, R. Xu, Z. Fang, A. Liu, BioResources 2014, 9,7 027 -7040.
- G .D eF alco,S .S imeone,M .B aroli, J. Coastal Res. 2008, 24,69-75.
- B. H. Daru, K. Yessoufou in DNAB arcodingi nM arine Perspectives, Springer, Switzerland, 2016,pp. 313 -330.
- Y. A. Tsigie,C .-Y.W ang, N. S. Kasim,Q .-D.D iem, L.-H. Huynh, Q.-P.H o, C.-T.T ruong, Y. -H. Ju, BioMed Res.I nt. 2012, 2012, 378384;
- L. Yong-Hong, L. Bo,Z .Z ong-Bao,B .F eng-Wu, Chin. J. Biotechnol. 2006, 22,6 50 -656.
- Energy Technol. 2018, 6, 1026-1038 2018 The .ublished -VCH . ,W einheim
- Z. Gong, H. Shen, X.Y ang, Q. Wang, H. Xie,Z .K.Z hao, Biotech- nol. Biofuels 2014, 7,158.
- Y. Zha, B. Muilwijk,L .C oulier, P. J. Punt, J. Bioprocess.B iotech. 2012, 2,1 12 -122.
- S. Asif,L .F .C huah, J. J. Klemes ˇ,M .A hmad, M. M. Akbar, K. T. Lee,A.F atima, J. Cleaner Prod. 2017, 161,1360 -1373.
- B. Díez, C. Pedrós-Alió,R .M assana, Appl. Environ. Microbiol. 2001, 67,2932 -2941.
- C. Kurtzman, C. Robnett, J. Clin. Microbiol. 1997, 35,1 216 -1223.
- J. Folch, M. Lees,G .S .S tanley, J. Biol. Chem. 1957, 226,4 97 -509.
Cited by
Sorted by citations
The potential of biofuels from first to fourth generation
Cavelius, P., Engelhart-Straub, S., Mehlmer, N., Lercher, J., Awad, D., & Brück, T. (2023) · PLOS Biology · doi:10.1371/journal.pbio.3002063 · Cited by 224 · see citing works
Self-citationMasri, M. A., Garbe, D., Mehlmer, N., & Brück, T. B. (2019) · Energy & Environmental Science · doi:10.1039/c9ee00210c · Cited by 98 · see citing works
Self-citationYounes, S., Bracharz, F., Awad, D., Qoura, F., Mehlmer, N., & Brueck, T. (2020) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-020-02354-0 · Cited by 54 · see citing works
Self-citationIdentifying carbohydrate-active enzymes of Cutaneotrichosporon oleaginosus using systems biology
Fuchs, T., Melcher, F., Rerop, Z. S., Lorenzen, J., Shaigani, P., Awad, D., Haack, M., Prem, S. A., Masri, M., Mehlmer, N., et al. (2021) · Microbial Cell Factories · doi:10.1186/s12934-021-01692-2 · Cited by 22 · see citing works
Self-citationAwad, D., Younes, S., Glemser, M., M. Wagner, F., Schenk, G., Mehlmer, N., & Brueck, T. (2020) · Sustainable Energy & Fuels · doi:10.1039/d0se00540a · Cited by 15 · see citing works
Self-citation
- Authors
- Masri, M. A., Younes, S., Haack, M., Qoura, F., Mehlmer, N., & Brück, T.
- Affiliations
- Technical University of Munich (DE)
- Corresponding author
- Brück, Thomas
- Venue
- Energy Technology
- Publisher
- Wiley
- Published
- 2017-12-11
- Volume
- 6
- Issue
- 6
- Pages
- 1026-1038
- ISSN
- 2194-4288 (ISSN-L)
- 2194-4296
- OpenAlex
- W2757757252
- Cited by
- 41
- References
- 64
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 7 · 2023: 5 · 2022: 3 · 2021: 17 · 2020: 3 · 2019: 1
- FWCI
- 0.49
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Energy Technology (publishedVersion, cc-by-nc-nd)
- Funders
- German Federal Ministry of Education and Research (031A261, 03SF0446A)
- Bavarian State Ministry for Environment and Consumer Protection (TLKO1U-69045)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- seagrass, biorefinery, single cell oil, microbial lipids, oleaginous yeast, enzymatic hydrolysis, carbohydrate content, Zostera marina, Zostera noltii, Posidonia oceanica, Thalassia testudinum, biofuels, oleochemicals, 18S rRNA phylogeny
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
