Towards high-throughput optimization of microbial lipid production: from strain development to process monitoring

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751 · see citing works

Biodiesel production from oleaginous microorganisms

Meng, X., Yang, J., Xu, X., Zhang, L., Nie, Q., & Xian, M. (2008) · Renewable Energy · doi:10.1016/j.renene.2008.04.014 · Cited by 1,155 · see citing works

A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae

Chen, W., Zhang, C., Song, L., Sommerfeld, M., & Hu, Q. (2009) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2009.01.001 · Cited by 681 · see citing works

Systems strategies for developing industrial microbial strains

Lee, S. Y., & Kim, H. U. (2015) · Nature Biotechnology · doi:10.1038/nbt.3365 · Cited by 589 · see citing works

Lipids of oleaginous yeasts. Part II: Technology and potential applications

Papanikolaou, S., & Aggelis, G. (2011) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201100015 · Cited by 389 · see citing works

Optimizing antenna size to maximize photosynthetic efficiency.

Ort, D. R., Zhu, X., (朱新广), & Melis, A. (2011) · Plant Physiology · doi:10.1104/pp.110.165886 · Cited by 338 · see citing works

Genes required for ionizing radiation resistance in yeast

Bennett, C. B., Lewis, L. K., Karthikeyan, G., Lobachev, K. S., Jin, Y. H., Sterling, J. F., Snipe, J. R., & Resnick, M. A. (2001) · Nature Genetics · doi:10.1038/ng778 · Cited by 328 · see citing works

Single cell oil production from low-cost substrates: The possibility and potential of its industrialization

Huang, C., Chen, X., Xiong, L., Chen, X.-D., Ma, L., & Chen, Y. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.08.010 · Cited by 305 · see citing works

The Engineering Potential of Rhodosporidium toruloides as a Workhorse for Biotechnological Applications

Park, Y. K., Nicaud, J., & Ledesma‐Amaro, R. (2017) · Trends in biotechnology · doi:10.1016/j.tibtech.2017.10.013 · Cited by 233 · see citing works

Modeling and optimization of biogas production on saw dust and other co-substrates using Artificial Neural network and Genetic Algorithm

Kana, E. B. G., Oloke, J. K., Lateef, A., et al. (2012) · Renewable Energy · doi:10.1016/j.renene.2012.03.027 · Cited by 211 · see citing works

An improved high-throughput Nile red fluorescence assay for estimating intracellular lipids in a variety of yeast species

Sitepu, I. R., Ignatia, L., Franz, A. K., Wong, D. M., Faulina, S. A., Tsui, M., Kanti, A., & Boundy-Mills, K. (2012) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2012.09.001 · Cited by 180 · see citing works

Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae

Chen, W., Sommerfeld, M., & Hu, Q. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2010.06.076 · Cited by 180 · see citing works

An evolutionary metabolic engineering approach for enhancing lipogenesis in Yarrowia lipolytica

Liu, L., Pan, A., Spofford, C., Zhou, N., & Alper, H. S. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.02.003 · Cited by 150 · see citing works

Grand Research Challenges for Sustainable Industrial Biotechnology

Straathof, A. J. J., Wahl, A., Benjamin, K. R., Takors, R., Wierckx, N. J. P., & Noorman, H. J. (2019) · Trends in biotechnology · doi:10.1016/j.tibtech.2019.04.002 · Cited by 147 · see citing works

Biotechnology, Big Data and Artificial Intelligence

Oliveira, A. L. (2019) · Biotechnology Journal · doi:10.1002/biot.201800613 · Cited by 145 · see citing works

Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools

Al-Khayri, J. M., Jain, S. M., & Johnson, D. V. (2015) · Springer eBooks · doi:10.1007/978-3-319-22521-0 · Cited by 131 · see citing works

Enhanced intracellular lipid in Nannochloropsis sp. via random mutagenesis and flow cytometric cell sorting

Doan, Y. T.-T., & Obbard, J. P. (2012) · Algal Research · doi:10.1016/j.algal.2012.03.001 · Cited by 120 · see citing works

Optimization of carbon and nitrogen sources for biomass and lipid production by Chlorella saccharophila under heterotrophic conditions and development of Nile red fluorescence based method for quantification of its neutral lipid content

Isleten-Hosoglu, M., Gultepe, I., & Elibol, M. (2012) · Biochemical Engineering Journal · doi:10.1016/j.bej.2011.12.001 · Cited by 114 · see citing works

The development and application of high throughput cultivation technology in bioprocess development

Long, Q., Liu, X., Yang, Y., et al. (2014) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2014.03.028 · Cited by 111 · see citing works

Evolution of Mutation Rates: Phylogenomic Analysis of the Photolyase/Cryptochrome Family

Lucas‐Lledó, J. I., & Lynch, M. (2009) · Molecular Biology and Evolution · doi:10.1093/molbev/msp029 · Cited by 111 · see citing works

Application of the standard addition method for the absolute quantification of neutral lipids in microalgae using Nile red

Bertozzini, E., Galluzzi, L., Penna, A., & Magnani, M. (2011) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2011.06.018 · Cited by 109 · see citing works

High-Throughput Screening Techniques for Biomass Conversion

Decker, S. R., Brunecky, R., Tucker, M. P., et al. (2009) · BioEnergy Research · doi:10.1007/s12155-009-9051-0 · Cited by 100 · see citing works

A sustainable, high-performance process for the economic production of waste-free microbial oils that can replace plant-based equivalents.

Masri, M. A., Garbe, D., Mehlmer, N., & Brück, T. B. (2019) · Energy & Environmental Science · doi:10.1039/c9ee00210c · Cited by 98 · see citing works

Optimization of lipid production by the oleaginous yeast Lipomyces starkeyi by random mutagenesis coupled to cerulenin screening

Tapia, E. V., Anschau, A., Coradini, A. L. V., Franco, T. T., & Deckmann, A. C. (2012) · AMB Express · doi:10.1186/2191-0855-2-64 · Cited by 91 · see citing works

Enzymatic hydrolysis of sugarcane bagasse for bioethanol production: determining optimal enzyme loading using neural networks

Rivera, E. C., Rabelo, S. C., Garcia, D. D. R., et al. (2010) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.2391 · Cited by 82 · see citing works

Multiplexed CRISPR-Cas9-Based Genome Editing of Rhodosporidium toruloides

Otoupal, P. B., Ito, M., Arkin, A. P., Magnuson, J. K., Gladden, J. M., & Skerker, J. M. (2019) · mSphere · doi:10.1128/msphere.00099-19 · Cited by 79 · see citing works

Mutants of Yarrowia lipolytica NCIM 3589 grown on waste cooking oil as a biofactory for biodiesel production

Katre, G., Ajmera, N., Zinjarde, S. S., et al. (2017) · Microbial Cell Factories · doi:10.1186/s12934-017-0790-x · Cited by 78 · see citing works

Breeding of high biomass and lipid producing Desmodesmus sp. by Ethylmethane sulfonate-induced mutation

Zhang, Y., He, M., Zou, S., Fei, C., Yan, Y., Zheng, S., Ahmed, R. A., & Wang, C. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.01.120 · Cited by 76 · see citing works

Genomics and Transcriptomics Analyses of the Oil-Accumulating Basidiomycete Yeast Trichosporon oleaginosus : Insights into Substrate Utilization and Alternative Evolutionary Trajectories of Fungal Mating Systems

Kourist, R., Bracharz, F., Lorenzen, J., Kracht, O. N., Chovatia, M., Daum, C. G., Deshpande, S., Lipzen, A., Nolan, M., Ohm, R. A., et al. (2015) · mBio · doi:10.1128/mbio.00918-15 · Cited by 74 · see citing works

Identification of oleaginous yeast strains able to accumulate high intracellular lipids when cultivated in alkaline pretreated corn stover

Sitepu, I. R., Jin, M., Fernandez, J. E., Sousa, L. D. C., Balan, V., & Boundy‐Mills, K. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-5944-8 · Cited by 74 · see citing works

Multi-Factorial-Guided Media Optimization for Enhanced Biomass and Lipid Formation by the Oleaginous Yeast Cutaneotrichosporon oleaginosus

Awad, D., Bohnen, F., Mehlmer, N., & Brueck, T. (2019) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00054 · Cited by 73 · see citing works

Genetic engineering and production of modified fatty acids by the non-conventional oleaginous yeast Trichosporon oleaginosus ATCC 20509

Görner, C., Redai, V., Bracharz, F., Schrepfer, P., Garbe, D., & Brück, T. (2015) · Green Chemistry · doi:10.1039/c5gc01767j · Cited by 68 · see citing works

The Optimal Mutagen Dosage to Induce Point-Mutations in Synechocystis sp. PCC6803 and Its Application to Promote Temperature Tolerance

Tillich, U. M., Lehmann, S., Schulze, K., Dühring, U., & Frohme, M. (2012) · PLoS ONE · doi:10.1371/journal.pone.0049467 · Cited by 67 · see citing works

Utilization of sorghum bagasse hydrolysates for producing microbial lipids

Liang, Y., Tang, T., Umagiliyage, A. L., Siddaramu, T., McCarroll, M. E., & Choudhary, R. (2011) · Applied Energy · doi:10.1016/j.apenergy.2011.10.013 · Cited by 67 · see citing works

Estimation of neutral lipid levels in Antarctic sea ice microalgae by nile red fluorescence

Priscu, J. C., Priscu, L. R., Palmisano, A. C., & Sullivan, C. W. (1990) · Antarctic Science · doi:10.1017/s0954102090000190 · Cited by 65 · see citing works

A quick isolation method for mutants with high lipid yield in oleaginous yeast

Wang, J., Li, R., Lü, D., Ma, S., Yan, Y., & Li, W. (2009) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-009-9960-2 · Cited by 64 · see citing works

Opportunities and challenges in the development of Cutaneotrichosporon oleaginosus ATCC 20509 as a new cell factory for custom tailored microbial oils

Bracharz, F., Beukhout, T., Mehlmer, N., & Brück, T. (2017) · Microbial Cell Factories · doi:10.1186/s12934-017-0791-9 · Cited by 64 · see citing works

Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement

Kumawat, S., Rana, N., Bansal, R., et al. (2019) · Plants · doi:10.3390/plants8060164 · Cited by 58 · see citing works

Improving the reliability of fluorescence-based neutral lipid content measurements in microalgal cultures

De la Hoz Siegler, H., Ayidzoe, W., Ben-Zvi, A., Burrell, R. E., & McCaffrey, W. C. (2012) · Algal Research · doi:10.1016/j.algal.2012.07.004 · Cited by 57 · see citing works

Simultaneous separation and detection of hepatitis A virus and norovirus in produce

Morales-Rayas, R., Wolffs, P. F. G., & Griffiths, M. W. (2010) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2010.02.011 · Cited by 56 · see citing works

Improvement of lipid production by the oleaginous yeast Rhodosporidium toruloides through UV mutagenesis

Yamada, R., Kashihara, T., & Ogino, H. (2017) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-017-2269-7 · Cited by 56 · see citing works

Plantwide Optimizing Control of a continuous bioethanol production process

Ochoa, S., Wozny, G., & Repke, J. (2010) · Journal of Process Control · doi:10.1016/j.jprocont.2010.06.010 · Cited by 54 · see citing works

Improvement of lipid production in oleaginous yeast Rhodosporidium toruloides by ultraviolet mutagenesis

Guo, M., Cheng, S., Chen, G., & Chen, J. (2019) · Engineering in Life Sciences · doi:10.1002/elsc.201800203 · Cited by 54 · see citing works

Microbial lipid production by oleaginous yeasts grown on Scenedesmus obtusiusculus microalgae biomass hydrolysate.

Younes, S., Bracharz, F., Awad, D., Qoura, F., Mehlmer, N., & Brueck, T. (2020) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-020-02354-0 · Cited by 54 · see citing works

Continuous directed evolution for strain and protein engineering

d’Oelsnitz, S., & Ellington, A. D. (2018) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2017.12.020 · Cited by 53 · see citing works

Constructive learning neural network applied to identification and control of a fuel-ethanol fermentation process

Meleiro, L. A. D. C., Zuben, F. J. V., & Filho, R. M. (2008) · Engineering Applications of Artificial Intelligence · doi:10.1016/j.engappai.2008.06.001 · Cited by 51 · see citing works

Lipid Modification by Enzymes and Engineered Microbes

(2018) · Elsevier eBooks · doi:10.1016/c2016-0-04104-9 · Cited by 51 · see citing works

Neutron-induced Adaptive Response Studied in Go Human Lymphocytes Using the Comet Assay

Gajendiran, N., Tanaka, K. J., KUMARAVEL, T. S., et al. (2001) · Journal of Radiation Research · doi:10.1269/jrr.42.91 · Cited by 49 · see citing works

A Comparative Study of Rejoining of DNA Double-strand Breaks in Yeast Irradiated with 3·5 MeV α-particles or with 30 MeV Electrons

Frankenberg-Schwager, M., Frankenberg, D., Harbich, R., et al. (1990) · International Journal of Radiation Biology · doi:10.1080/09553009014551261 · Cited by 48 · see citing works

Characterization of oleaginous yeasts revealed two novel species:Trichosporon cacaoliposimilissp. nov. andTrichosporon oleaginosussp. nov.

Gujjari, P., Suh, S., Coumes, K., & Zhou, J. J. (2011) · Mycologia · doi:10.3852/10-403 · Cited by 48 · see citing works

A new strategy for strain improvement of Aurantiochytrium sp. based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase

Cheng, Y., Sun, Z., Cui, G., Song, X., & Cui, Q. (2016) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2016.08.019 · Cited by 47 · see citing works

A waste-free, microbial oil centered cyclic bio-refinery approach based on flexible macroalgae biomass

Masri, M. A., Jurkowski, W., Shaigani, P., Haack, M., Mehlmer, N., & Brück, T. (2018) · Applied Energy · doi:10.1016/j.apenergy.2018.04.089 · Cited by 46 · see citing works

Advances in analytical tools for high throughput strain engineering

Marcellin, E., & Nielsen, L. K. (2018) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2018.01.027 · Cited by 43 · see citing works

A Seagrass-Based Biorefinery for Generation of Single-Cell Oils for Biofuel and Oleochemical Production.

Masri, M. A., Younes, S., Haack, M., Qoura, F., Mehlmer, N., & Brück, T. (2018) · Energy Technology · doi:10.1002/ente.201700604 · Cited by 41 · see citing works

Automation of a Nile red staining assay enables high throughput quantification of microalgal lipid production

Morschett, H., Wiechert, W., & Oldiges, M. (2016) · Microbial Cell Factories · doi:10.1186/s12934-016-0433-7 · Cited by 36 · see citing works

Induction and Subsequent Repair of DNA Damage by Fast Neutrons in Cultured Mammalian Cells

Sakai, K., Suzuki, S., Nakamura, N., et al. (1987) · Radiation Research · doi:10.2307/3576999 · Cited by 35 · see citing works

New kids on the block: emerging oleaginous yeast of biotechnological importance

Yaguchi, A., Rives, D., & Blenner, M. (2017) · AIMS Microbiology · doi:10.3934/microbiol.2017.2.227 · Cited by 35 · see citing works

Optimization of a Nile Red method for rapid lipid determination in autotrophic, marine microalgae is species dependent

Balduyck, L., Veryser, C., Goiris, K., Bruneel, C., Muylaert, K., & Foubert, I. (2015) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2015.09.009 · Cited by 31 · see citing works

The munich fission neutron therapy facility MEDAPP at the research reactor FRM II

Wagner, F. M., Kneschaurek, P., Kastenmüller, A., et al. (2008) · Strahlentherapie und Onkologie · doi:10.1007/s00066-008-1878-3 · Cited by 30 · see citing works

Optimization of protein isolation by proteomic qualification from Cutaneotrichosporon oleaginosus

Awad, D., & Brueck, T. (2019) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-019-02254-7 · Cited by 30 · see citing works

Improvement of the Nile Red fluorescence assay for determination of total lipid content in microalgae independent of chlorophyll content

Ward, V. C. A., & Rehmann, L. (2014) · Journal of Applied Phycology · doi:10.1007/s10811-014-0481-5 · Cited by 27 · see citing works

Calculation of Biodiesel Fuel Characteristics Based on the Fatty Acid Composition of the Lipids of Some Biotechnologically Important Microorganisms

Sergeeva, Y. E., Mostova, E. B., Gorin, K. V., Komova, A. V., Konova, I. A., Pozhidaev, V. M., Gotovtsev, P. M., Vasilov, R. G., & Sineoky, S. P. (2017) · Applied Biochemistry and Microbiology · doi:10.1134/s0003683817080063 · Cited by 26 · see citing works

Identification of novel genes in the carotenogenic and oleaginous yeast Rhodotorula toruloides through genome-wide insertional mutagenesis

Liu, Y., Koh, C. M. J., Yap, S. A., Du, M., Hlaing, M. M., & Ji, L. (2018) · BMC Microbiology · doi:10.1186/s12866-018-1151-6 · Cited by 25 · see citing works

The Slower Cellular Recovery after Higher-LET Irradiations, Including Neutrons, Focuses on the Quality of DNA Breaks

Hendry, J. H. (1991) · Radiation Research · doi:10.2307/3578011 · Cited by 21 · see citing works

Dose–response relationship of dicentric chromosomes in human lymphocytes obtained for the fission neutron therapy facility MEDAPP at the research reactor FRM II

Schmid, E. W., Wagner, F. M., Romm, H., et al. (2008) · Radiation and Environmental Biophysics · doi:10.1007/s00411-008-0202-z · Cited by 15 · see citing works

Biofuels from Microbial Lipids

Chew, K. W., Chia, S. R., Show, P. L., Ling, T. C., & Chang, J. (2018) · Green energy and technology · doi:10.1007/978-981-10-7677-0_9 · Cited by 13 · see citing works

An integrated robotic system for high‐throughput process development of cell and virus culture conditions: Application to biosafety level 2 live virus vaccines

Daniels, C. L., Rodriguez, J., Lim, E., et al. (2015) · Engineering in Life Sciences · doi:10.1002/elsc.201400245 · Cited by 11 · see citing works

Enhancement of lipid productivity in green microalgae Chlorella sp. via fast neutron irradiation

Liu, S., Xu, J., Chen, W., Fu, H., Ma, L. Y., Xu, H. G., Xinnian, L., Wu, M., & Ma, F. (2016) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2016.05.013 · Cited by 11 · see citing works

The Relative Biological Effectiveness of 14.5-MeV Neutrons for the Induction of Gene Conversion and Mutation in Yeast

Unrau, P. (1986) · Radiation Research · doi:10.2307/3576848 · Cited by 9 · see citing works

Systematic Optimization of a Robust Telescoped Process for a BTK Inhibitor with Atropisomer Control by High-Throughput Experimentation, Design of Experiments, and Linear Regression

Gonzalez, F. L., Wisniewski, S. R., Katipally, K. R., et al. (2019) · Organic Process Research & Development · doi:10.1021/acs.oprd.8b00398 · Cited by 8 · see citing works

Etude de stratégies de culture de Dunaliella tertiolecta combinant haute densité cellulaire et accumulation de lipides en vue de produire du biodiesel

Massart, A., Aubry, É., & Hantson, A. (2009) · Flanders Marine Institute (Flanders Marine Institute) · Cited by 7 · see citing works

DOSIMETRY WITH SALT IN MIXED RADIATION FIELDS OF PHOTONS AND NEUTRONS

Ekendahl, D., Rubovič, P., Žlebčík, P., et al. (2017) · Radiation Protection Dosimetry · doi:10.1093/rpd/ncx114 · Cited by 6 · see citing works

The potential of biofuels from first to fourth generation

Cavelius, P., Engelhart-Straub, S., Mehlmer, N., Lercher, J., Awad, D., & Brück, T. (2023) · PLOS Biology · doi:10.1371/journal.pbio.3002063 · Cited by 224 · see citing works

Oleaginous yeasts- substrate preference and lipid productivity: a view on the performance of microbial lipid producers

Shaigani, P., Awad, D., Redai, V., Fuchs, M., Haack, M., Mehlmer, N., & Brueck, T. (2021) · Microbial Cell Factories · doi:10.1186/s12934-021-01710-3 · Cited by 68 · see citing works

Yeasts and Yeast-like Organisms

Kockova-Kratichvilova, A., & Kocková‐Kratochvílová, A. (1990) · Medical Entomology and Zoology · Cited by 74 · see citing works

Yeasts

Athnasios, A. K., & Quantz, M. (2012) · Ullmann's Encyclopedia of Industrial Chemistry · doi:10.1002/14356007.a28_461.pub2 · Cited by 1 · see citing works

Formation by Yeast of the HEMF (4-hydroxy-2(or 5)-ethyl-5(or 2)-methyl-3(2H)-furanone) Aroma Component in Miso with Aging

Sugawara, E., Hashimoto, S., Sakurai, Y., et al. (1994) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.58.1134 · Cited by 40 · see citing works

Metabolic Activity of the Dehydrated Yeast by Spray Drying

Márquez-Montes, R., Robles-Olvera, V. J., Luna‐Solano, G., et al. (2007) · Drying Technology · doi:10.1080/07373930701438824 · Cited by 8 · see citing works

Effects of Yeast Properties on Beer Flavoring Substances

Zhi-jian, W. (2008) · Liquor-making Science & Technology