Dose-dependent effects of kefir on colitis induced by trinitrobenzene sulfonic acid in rats
Open accessAbstract
Evidence suggests that gut microbiota dysbiosis plays a critical role in the initiation and promotion of inflammatory bowel disease (IBD). Kefir is a fermented dairy product including yeast and bacterial species. We aimed to investigate the effect of kefir on trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats using two different doses. Fifty-four Wistar rats were divided into six groups. For 14 days, the normal control and colitis control groups were given tap water, kefir10 control, kefir10 colitis, and kefir30 control, and the kefir30 colitis groups were given phosphate-buffered saline containing 10% or 30% kefir, respectively, instead of tap water. Colitis was induced by intracolonically administrating TNBS in the colitis control, kefir10 colitis, and kefir30 colitis groups. On the 14th day, the rats were sacrificed. The weights and lengths of the colons were measured and macroscopically evaluated, and the distal 10 cm segments were subjected to a histopathological examination. The incidence of bloody stool and diarrhea in the kefir10 colitis group was found to be less than the colitis control and kefir30 colitis groups. The colonic weight/length ratio in the kefir10 colitis group was lower than that in the colitis control and kefir30 colitis groups. We detected that the 10% kefir treatment reduced TNBS-induced macroscopic colonic damage, while it was exacerbated by the 30% kefir treatment. No significant difference was observed between the colitis groups in terms of microscopic colonic damage scoring. These results indicate that kefir, with a careful dose selection, may be a useful agent in the treatment of IBD.
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Kefir contains bacteria such as L. acidophilus, brevis, casei, delbrueckii subsp. Bulgaricus, and yeasts such as Candida humilis, Kazachstania unispora, and Kluyveromyces lactis.
“It is a fermented milk drink that contains bacteria, such as L. acidophilus, brevis, casei, delbrueckii subsp. Bulgaricus, and yeasts such as Candida humilis, Kazachstania unispora, and Kluyveromyces lactis [190].”
From The Potential Impact of Probiotics on Human Health: An Update on Their Health-Promoting Properties (2024) · cites this paper as [190]
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- Authors
- Sevencan, N. O., Isler, M., Kapucuoglu, F. N., Senol, A., Kayhan, B., Kiztanir, S., & Kockar, M. C.
- Affiliations
- Department of Internal Medicine, Medical Faculty The University of Karabuk Karabuk Turkey
- Süleyman Demirel Üniversitesi (TR)
- Department of Pathology, Medical Faculty The University of Suleyman Demirel Isparta Turkey
- Department of Gastroenterology, Medical Faculty The University of Suleyman Demirel Isparta Turkey
- Department of Internal Medicine, Medical Faculty The University of Suleyman Demirel Isparta Turkey
- ORCID
- Venue
- Food Science & Nutrition
- Publisher
- Wiley
- Published
- 2019-08-22
- Volume
- 7
- Issue
- 9
- Pages
- 3110-3118
- ISSN
- 2048-7177 (ISSN-L)
- PMC
- PMC6766543
- OpenAlex
- W2969780477
- Cited by
- 22
- References
- 31
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 5 · 2023: 4 · 2022: 8 · 2021: 2 · 2019: 1
- FWCI
- 1.66
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Food Science & Nutrition (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Digital Collections portal (Koç University) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Informationszentrum Lebenswissenschaften (ZB MED) (submittedVersion, cc-by)
- Funders
- Suleyman Demirel University Research Projects Management Unit (1303-TU-06)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- kefir, trinitrobenzene sulfonic acid, colitis, inflammatory bowel disease, dose-dependent effects, gut dysbiosis, fermented dairy products, colonic damage
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