The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
In situ detection of fragmented dna (tunel assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death: A cautionary note
Open accessAbstract
Detection of DNA fragments in situ using the terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labeling (TUNEL) assay is increasingly applied to investigate active cell death (apoptosis). We studied the specificity of the assay in well-defined models of apoptosis and necrosis as well as in postmortem autolysis in rat liver. During involution of liver hyperplasia, which follows stopping treatment with the hepatomitogens cyproterone acetate (CPA) or nafenopin (NAF), numerous apoptotic hepatocytes could be observed with TUNEL-positive chromatin residues. A similar TUNEL-positive reaction appeared in necrotic hepatocytes after a cytotoxic dose of carbon tetrachloride (CCl 4) or N-nitrosomorpholine (NNM). Also, in insufficiently fixed, autolytic livers TUNEL-positive nuclei were observed. Thus, DNA fragmentation is common to different kinds of cell death; its detection in situ should not be considered a specific marker of apoptosis.
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A Nonapoptotic Cell Death Process, Entosis, that Occurs by Cell-in-Cell Invasion
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Photoreceptor degeneration: genetic and mechanistic dissection of a complex trait
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Detection of Apoptosis by TUNEL Assay
Kyrylkova, K., Kyryachenko, S., Leid, M., & Kioussi, C. (2012) · Methods in Molecular Biology · doi:10.1007/978-1-61779-860-3_5 · Cited by 510
Song, M., Mihara, K., Chen, Y., Scorrano, L., & Dorn, G. W. (2015) · Cell Metabolism · doi:10.1016/j.cmet.2014.12.011 · Cited by 482
Metadata
- Authors
- Kraupp, B. G., Ruttkay-Nedecky, B., Koudelka, H., Bukowska, K., Bursch, W., & Schulte-Hermann, R.
- Venue
- Hepatology
- Published
- 1995-05
- Volume
- 21
- Issue
- 5
- Pages
- 1465-1468
- ISSN
- 0270-9139 (ISSN-L)
- OpenAlex
- W2025955916
- Cited by
- 1,156
- References
- 31
- FWCI
- 15.16
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: In situ detection of fragmented dna (tunel assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death: A cautionary note. https://app.cochat.ai/papers/10.1002/hep.1840210534
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- Kraupp, B. G., Ruttkay-Nedecky, B., Koudelka, H., Bukowska, K., Bursch, W., & Schulte-Hermann, R. (1995). In situ detection of fragmented dna (tunel assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death: A cautionary note. Hepatology. https://doi.org/10.1002/hep.1840210534
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