Lethal effect of the anti-Fas antibody in mice
Abstract
During mammalian development, many cells are programmed to die most mediated by apoptosis. The Fas antigen coded by the structural gene for mouse lymphoproliferation mutation (lpr), is a cell surface protein belonging to the tumour necrosis factor/nerve growth factor receptor family, and mediates apoptosis. The Fas antigen messenger RNA is expressed in the thymus, liver, heart, lung and ovary. We prepared a monoclonal antibody against mouse Fas antigen, which immunoprecipitated the antigen (M(r) 45K) and had cytolytic activity against cell lines expressing mouse Fas antigen. We report here that staining of mouse thymocytes with the antibody indicated that thymocytes from the wild-type and lprcg mice expressed the Fas antigen, whereas little expression of the Fas antigen was found in lpr mice. Intraperitoneal administration of the anti-Fas antibody into mice rapidly killed the wild-type mice but neither lpr nor lprcg mice. Biochemical, histological and electron microscope analyses indicated severe damage of the liver by apoptosis. These findings suggest that the Fas antigen is important in programmed cell death in the liver, and may be involved in fulminant hepatitis in some cases.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Fas-mediated apoptosis is also reduced, as judged by using the monoclonal antibody Jo2 that recognizes and activates the Fas receptor and induces hepatic apoptosis, or by using ethanol gavage in mice, a system that mimics binge drinking in humans and triggers FasL-induced hepatocyte apoptosis.
“Fas-mediated apoptosis is also reduced, as judged by using the monoclonal antibody Jo2 that recognizes and activates the Fas receptor and induces hepatic apoptosis [109], or by using ethanol gavage in mice, a system that mimics binge drinking in humans and triggers FasL-induced hepatocyte apoptosis [110].”
From CCN1/CYR61: the very model of a modern matricellular protein (2011) · cites this paper as [109]
Cites
Cell Death: The Significance of Apoptosis
Wyllie, A. H., Kerr, J. F. R., & Currie, A. R. (1980) · International review of cytology · doi:10.1016/s0074-7696(08)62312-8 · Cited by 7,235 · see citing works
Current Protocols in Immunology
(2018) · Current Protocols in Immunology · doi:10.1002/(issn)1934-368x · Cited by 3,574 · see citing works
Lymphoproliferation disorder in mice explained by defects in Fas antigen that mediates apoptosis
Watanabe‐Fukunaga, R., Brannan, C., Copeland, N., Jenkins, N., & Nagata, S. (1992) · Nature · doi:10.1038/356314a0 · Cited by 3,153 · see citing works
Self-citationThe polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis
Itoh, N., Yonehara, S., Ishii, A., Yonehara, M., Mizushima, S.-I., Sameshima, M., Hase, A., Seto, Y., & Nagata, S. (1991) · Cell · doi:10.1016/0092-8674(91)90614-5 · Cited by 2,736 · see citing works
Self-citationSocial controls on cell survival and cell death
RAFF, M. C. (1992) · Nature · doi:10.1038/356397a0 · Cited by 2,634 · see citing works
Shock and Tissue Injury Induced by Recombinant Human Cachectin
Tracey, K. J., Beutler, B., Lowry, S. F., Merryweather, J., Wolpe, S., Milsark, I. W., Hariri, R. J., Fahey, T. J., Zentella, A., Albert, J. D., et al. (1986) · Science · doi:10.1126/science.3764421 · Cited by 2,549 · see citing works
Mechanisms and Functions of Cell Death
Ellis, R. E., Yuan, J., & Horvitz, H. R. (1991) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.07.110191.003311 · Cited by 2,063 · see citing works
Monoclonal Antibody-Mediated Tumor Regression by Induction of Apoptosis
Trauth, B. C., Klas, C., Peters, A. M. J., Matzku, S., Mӧller, P., Falk, W., Debatin, K., & Krammer, P. H. (1989) · Science · doi:10.1126/science.2787530 · Cited by 1,716 · see citing works
Current protocols in immunology
Nezlin, R. S. (1992) · Molecular Immunology · doi:10.1016/0161-5890(92)90114-d · Cited by 1,413 · see citing works
Yonehara, S., Ishii, A., & Yonehara, M. (1989) · The Journal of Experimental Medicine · doi:10.1084/jem.169.5.1747 · Cited by 1,398 · see citing works
Oehm, A., Behrmann, I., Falk, W., Pawlita, M., Maier, G., Klas, C., Li-Weber, M., Richards, S., Dhein, J., & Trauth, B. C. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50076-x · Cited by 834 · see citing works
The cDNA structure, expression, and chromosomal assignment of the mouse Fas antigen
Watanabe‐Fukunaga, R., Brannan, C. I., Itoh, N., Yonehara, S., Copeland, N. G., Jenkins, N. A., & Nagata, S. (1992) · The Journal of Immunology · doi:10.4049/jimmunol.148.4.1274 · Cited by 815 · see citing works
Self-citationFas involvement in Ca(2+)-independent T cell-mediated cytotoxicity.
Rouvier, É., Luciani, M. F., & Golstein, P. (1993) · The Journal of Experimental Medicine · doi:10.1084/jem.177.1.195 · Cited by 799 · see citing works
Apoptosis and the regulation of cell numbers in normal and neoplastic tissues: an overview
Wyllie, A. H. (1992) · Cancer and Metastasis Reviews · doi:10.1007/bf00048057 · Cited by 588 · see citing works
Lehmann, V., Freudenberg, M. A., & Galanos, C. (1987) · The Journal of Experimental Medicine · doi:10.1084/jem.165.3.657 · Cited by 547 · see citing works
Adachi, M., Watanabe‐Fukunaga, R., & Nagata, S. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.5.1756 · Cited by 509 · see citing works
Self-citationThe significance of spontaneous and induced apoptosis in the gastrointestinal tract of mice
Potten, C. S. (1992) · Cancer and Metastasis Reviews · doi:10.1007/bf00048063 · Cited by 331 · see citing works
Eskandari, M. K., Bolgos, G. L., Miller, C., Nguyen, D. T., DeForge, L. E., & Remick, D. G. (1992) · The Journal of Immunology · doi:10.4049/jimmunol.148.9.2724 · Cited by 320 · see citing works
The Genetic Mapping of a Defective Lps Response Gene in C3H/HeJ Mice
Watson, J. D., Kelly, K., Largen, M. T., & Taylor, B. A. (1978) · The Journal of Immunology · doi:10.4049/jimmunol.120.2.422 · Cited by 277 · see citing works
Interleukin 1 potentiates the lethal effect of tumor necrosis factor alpha/cachectin in mice.
Waage, A., & Espevik, T. (1988) · The Journal of Experimental Medicine · doi:10.1084/jem.167.6.1987 · Cited by 242 · see citing works
Characterization of IL-6 receptor expression by monoclonal and polyclonal antibodies.
Hirata, Y., Taga, T., Hibi, M., Nakano, N., Hirano, T., & Kishimoto, T. (1989) · The Journal of Immunology · doi:10.4049/jimmunol.143.9.2900 · Cited by 175 · see citing works
Monoclonal-antibody-mediated apoptosis in adult T-cell leukaemia
Debatin, K., Krammer, P. H., Goldman, C. K., Bamford, R. N., & Waldmann, T. A. (1990) · The Lancet · doi:10.1016/0140-6736(90)90735-n · Cited by 140 · see citing works
Kobayashi, N., Hamamoto, Y., Yamamoto, N., Ishii, A., Yonehara, M., & Yonehara, S. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.24.9620 · Cited by 118 · see citing works
Meier, T., Arni, S., Malarkannan, S., Poincelet, M., & Hoessli, D. C. (1992) · Analytical Biochemistry · doi:10.1016/0003-2697(92)90165-4 · Cited by 97 · see citing works
Falk, M., Trauth, B. C., Debatin, K., Klas, C., Gregory, C., Rickinson, A. B., Calender, A., Lenoir, G., Ellwart, J., & Krammer, P. (1992) · Blood · doi:10.1182/blood.v79.12.3300.bloodjournal79123300 · Cited by 96 · see citing works
Preferential distribution of apoptotic bodies in acinar zone 3 of normal human and rat liver
Benedetti, A., Jézéquel, A. M., & Orlandi, F. (1988) · Journal of Hepatology · doi:10.1016/s0168-8278(88)80004-7 · Cited by 88 · see citing works
Sánchez‐Madrid, F., Szklut, P., & Springer, T. A. (1983) · The Journal of Immunology · doi:10.4049/jimmunol.130.1.309 · Cited by 41 · see citing works
Hepatitis B Virus Biology and Pathogenesis
Chisari, F. V. (1992) · Elsevier eBooks · doi:10.1016/b978-0-12-462002-5.50008-x · Cited by 37 · see citing works
Nephritogenicity of the lprcg gene on the MRL background.
Kimura, M., Ogata, Y., Shimada, K., et al. (1992) · PubMed · Cited by 13 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Nagata, S., & Golstein, P. (1995) · Science · doi:10.1126/science.7533326 · Cited by 3,979 · see citing works
Self-citationMartin, S. J., Reutelingsperger, C. P., McGahon, A. J., Rader, J. A., van Schie, R. C., LaFace, D. M., & Green, D. R. (1995) · The Journal of experimental medicine · doi:10.1084/jem.182.5.1545 · Cited by 2,824 · see citing works
Lymphoproliferative Disorders with Early Lethality in Mice Deficient in <i>Ctla-4</i>
Waterhouse, P., Penninger, J. M., Timms, E., Wakeham, A., Shahinian, A., Lee, K. P., Thompson, C. B., Griesser, H., & Mak, T. W. (1995) · Science · doi:10.1126/science.270.5238.985 · Cited by 2,766 · see citing works
Tumoricidal activity of tumor necrosis factor-related apoptosis-inducing ligand in vivo.
Walczak, H., Miller, R. E., Ariail, K., Gliniak, B., Griffith, T. S., Kubin, M., Chin, W., Jones, J., Woodward, A., Le, T., et al. (1999) · Nature Medicine · doi:10.1038/5517 · Cited by 2,497 · see citing works
Catalytic activity of the caspase-8–FLIPL complex inhibits RIPK3-dependent necrosis
Oberst, A., Dillon, C. P., Weinlich, R., McCormick, L. L., Fitzgerald, P., Pop, C., Hakem, R., Salvesen, G. S., & Green, D. R. (2011) · Nature · doi:10.1038/nature09852 · Cited by 1,278 · see citing works
Godoy, P., Hewitt, N. J., Albrecht, U., Andersen, M. E., Ansari, N., Bhattacharya, S., Bode, J. G., Bolleyn, J., Borner, C., Böttger, J., et al. (2013) · Archives of Toxicology · doi:10.1007/s00204-013-1078-5 · Cited by 1,280 · see citing works
Gross, A., Yin, X.-M., Wang, K., Wei, M. C., Jockel, J., Milliman, C., Erdjument-Bromage, H., Tempst, P., & Korsmeyer, S. J. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.2.1156 · Cited by 1,045 · see citing works
Bid-deficient mice are resistant to Fas-induced hepatocellular apoptosis
Yin, X.-M., Wang, K., Gross, A., Zhao, Y., Zinkel, S., Klocke, B., Roth, K. A., & Korsmeyer, S. J. (1999) · Nature · doi:10.1038/23730 · Cited by 1,004 · see citing works
The many roles of FAS receptor signaling in the immune system
Strasser, A., Jost, P. J., & Nagata, S. (2009) · Immunity · doi:10.1016/j.immuni.2009.01.001 · Cited by 968 · see citing works
Self-citationBcl‐2 and Fas/APO‐1 regulate distinct pathways to lymphocyte apoptosis.
Strasser, A., Harris, A. W., Huang, D. C., Krammer, P. H., & Cory, S. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb00304.x · Cited by 784 · see citing works
Hepatic T cells and liver tolerance
Crispe, I. N. (2003) · Nature Reviews Immunology · doi:10.1038/nri981 · Cited by 661 · see citing works
Exploring the TRAILs less travelled: TRAIL in cancer biology and therapy
von Karstedt, S., Montinaro, A., & Walczak, H. (2017) · Nature Reviews Cancer · doi:10.1038/nrc.2017.28 · Cited by 556 · see citing works
XIAP discriminates between type I and type II FAS-induced apoptosis
Jost, P. J., Grabow, S., Gray, D., McKenzie, M. D., Nachbur, U., Huang, D. C. S., Bouillet, P., Thomas, H. E., Borner, C., Silke, J., et al. (2009) · Nature · doi:10.1038/nature08229 · Cited by 482 · see citing works
Varanita, T., Soriano, M. E., Romanello, V., Zaglia, T., Quintana-Cabrera, R., Semenzato, M., Menabò, R., Costa, V., Civiletto, G., Pesce, P., et al. (2015) · Cell Metabolism · doi:10.1016/j.cmet.2015.05.007 · Cited by 460 · see citing works
Membrane-bound Fas ligand only is essential for Fas-induced apoptosis
O’ Reilly, L. A., Tai, L., Lee, L., Kruse, E. A., Grabow, S., Fairlie, W. D., Haynes, N. M., Tarlinton, D. M., Zhang, J.-G., Belz, G. T., et al. (2009) · Nature · doi:10.1038/nature08402 · Cited by 400 · see citing works
CCN1/CYR61: the very model of a modern matricellular protein
Lau, L. F. (2011) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-011-0778-3 · Cited by 338 · see citing works
Survival Function of the FADD-CASPASE-8-cFLIPL Complex
Dillon, C. P., Oberst, A., Weinlich, R., Janke, L. J., Kang, T.-B., Ben-Moshe, T., Mak, T. W., Wallach, D., & Green, D. R. (2012) · Cell Reports · doi:10.1016/j.celrep.2012.03.010 · Cited by 331 · see citing works
“As seen in caspase-8, RIPK3 DKO mice (Kaiser et al., 2011; Oberst et al., 2011), FADD, RIPK3 DKO mice were completely resistant to lethal hepatic injury induced by injection of agonistic anti-CD95 antibody (Ogasawara et al., 1993) (Figures 1C, S1B–”
BackgroundIncreased toxin-induced liver injury and fibrosis in interleukin-6-deficient mice
Kovalovich, K., DeAngelis, R. A., Li, W., Furth, E. E., Ciliberto, G., & Taub, R. (2000) · Hepatology · doi:10.1002/hep.510310123 · Cited by 298 · see citing works
Headen, D. M., Woodward, K. B., Coronel, M. M., Shrestha, P., Weaver, J. D., Zhao, H., Tan, M., Hunckler, M. D., Bowen, W. S., Johnson, C. T., et al. (2018) · Nature Materials · doi:10.1038/s41563-018-0099-0 · Cited by 175 · see citing works
Differential Regulation and Function of the Fas/Fas Ligand System in Human Trophoblast Cells1
Aschkenazi, S., Straszewski, S., Verwer, K. M. A., Foellmer, H., Rutherford, T., & Mor, G. (2002) · Biology of Reproduction · doi:10.1095/biolreprod66.6.1853 · Cited by 161 · see citing works
A Direct In Vivo RNAi Screen Identifies MKK4 as a Key Regulator of Liver Regeneration
Wuestefeld, T., Pesic, M., Rudalska, R., Dauch, D., Longerich, T., Kang, T.-W., Yevsa, T., Heinzmann, F., Hoenicke, L., Hohmeyer, A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.03.026 · Cited by 149 · see citing works
Ethanol-Induced Apoptosis in Mouse Liver
Zhou, Z., Sun, X., & Kang, Y. J. (2001) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)61699-9 · Cited by 129 · see citing works
Protein-based therapeutic approaches targeting death receptors
French, L. E., & Tschopp, J. (2003) · Cell Death & Differentiation · doi:10.1038/sj.cdd.4401185 · Cited by 102 · see citing works
Chabicovsky, M., Herkner, K., & Rossmanith, W. (2003) · Endocrinology · doi:10.1210/en.2003-0388 · Cited by 74 · see citing works
Human Hepatic Stellate Cells Inhibit T-Cell Response Through B7-H1 Pathway
Charles, R., Chou, H.-S., Wang, L., Fung, J. J., Lu, L., & Qian, S. (2013) · Transplantation · doi:10.1097/tp.0b013e318294caae · Cited by 72 · see citing works
Tada, K., Tanaka, M., Hanayama, R., Miwa, K., Shinohara, A., Iwamatsu, A., & Nagata, S. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.171.11.5718 · Cited by 70 · see citing works
Self-citationJuric, V., Chen, C.-C., & Lau, L. F. (2009) · Molecular and Cellular Biology · doi:10.1128/mcb.00064-09 · Cited by 70 · see citing works
Brodbeck, W. G., Shive, M. S., Colton, E., Ziats, N. P., & Anderson, J. M. (2002) · Journal of Laboratory and Clinical Medicine · doi:10.1067/mlc.2002.121260 · Cited by 52 · see citing works
Chen, C., & Lau, L. F. (2010) · Journal of Cell Communication and Signaling · doi:10.1007/s12079-009-0080-4 · Cited by 47 · see citing works
Ligand stimulation of CD95 induces activation of Plk3 followed by phosphorylation of caspase-8
Helmke, C., Raab, M., Rödel, F., Matthess, Y., Oellerich, T., Mandal, R., Sanhaji, M., Urlaub, H., Rödel, C., Becker, S., et al. (2016) · Cell Research · doi:10.1038/cr.2016.78 · Cited by 44 · see citing works
MOAP-1 Mediates Fas-Induced Apoptosis in Liver by Facilitating tBid Recruitment to Mitochondria
Tan, C. T., Zhou, Q.-L., Su, Y.-C., Fu, N. Y., Chang, H.-C., Tao, R. N., Sukumaran, S. K., Baksh, S., Tan, Y.-J., Sabapathy, K., et al. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.05.068 · Cited by 35 · see citing works
Therapeutics Targeting the Core Apoptotic Machinery
Hamilton, C., Fox, J. P., Longley, D. B., & Higgins, C. A. (2021) · Cancers · doi:10.3390/cancers13112618 · Cited by 26 · see citing works
The Extracellular Matrix Protein CCN1 Dictates TNFα and FasL Cytotoxicity In Vivo
Chen, C.-C., Juric, V., & Lau, L. F. (2011) · Advances in Experimental Medicine and Biology · doi:10.1007/978-1-4419-6612-4_63 · Cited by 8 · see citing works
- Authors
- Ogasawara, J., Watanabe-Fukunaga, R., Adachi, M., Matsuzawa, A., Kasugai, T., Kitamura, Y., Itoh, N., Suda, T., & Nagata, S.
- Affiliations
- Institute of Medical Science, The University of Tokyo (JP)
- The University of Osaka (JP)
- ORCID
- Nagata, Shigekazu
- Venue
- Nature
- Published
- 1993-08
- Volume
- 364
- Issue
- 6440
- Pages
- 806-809
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2067534266
- Cited by
- 1,881
- References
- 29
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 16 · 2023: 13 · 2022: 14 · 2021: 13 · 2020: 19 · 2019: 21 · 2018: 25 · 2017: 29 · 2016: 17 · 2015: 29 · 2014: 32 · 2013: 37 · 2012: 39
- FWCI
- 24.97
- Type
- journal article
- Language
- en
- Update notices
- correction (1993-10-07)
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- Fas antigen, anti-Fas antibody, apoptosis, programmed cell death, lpr mutation, thymocytes, liver injury, fulminant hepatitis, cytotoxicity, TNF receptor superfamily, mouse lethality
- MeSH terms
- Animals, Antibodies, Monoclonal, Antigens, Surface, Blood, Cell Line, Female, Cricetinae, Humans, Hybrid Cells, Liver, Mice, Inbred BALB C, Mice, Inbred C3H, Mutation, Myocardium, Phagocytosis, Thymus Gland, Apoptosis, fas Receptor, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
