Cited by 226Cancer-related Molecular PathwaysCancer Genomics and DiagnosticsGenomics and Rare Diseases

Analysis of TP53 Mutation Status in Human Cancer Cell Lines: A Reassessment

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Leroy, Bernard, Girard, Luc, Hollestelle, Antoinette, Minna, John D., Gazdar, Adi F., Soussi, Thierry·2014·Human Mutation

Abstract

Tumor-derived cell lines play an important role in the investigation of tumor biology and genetics. Across a wide array of studies, they have been tools of choice for the discovery of important genes involved in cancer and for the analysis of the cellular pathways that are impaired by diverse oncogenic events. They are also invaluable for screening novel anticancer drugs. The TP53 protein is a major component of multiple pathways that regulate cellular response to various types of stress. Therefore, TP53 status affects the phenotype of tumor cell lines profoundly and must be carefully ascertained for any experimental project. In the present review, we use the 2014 release of the UMD TP53 database to show that TP53 status is still controversial for numerous cell lines, including some widely used lines from the NCI-60 panel. Our analysis clearly confirms that, despite numerous warnings, the misidentification of cell lines is still present as a silent and neglected issue, and that extreme care must be taken when determining the status of p53, because errors may lead to disastrous experimental interpretations. A novel compendium gathering the TP53 status of 2,500 cell lines has been made available (http://p53.fr). A stand-alone application can be used to browse the database and extract pertinent information on cell lines and associated TP53 mutations. It will be updated regularly to minimize any scientific issues associated with the use of misidentified cell lines (http://p53.fr).

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Authors
Leroy, B., Girard, L., Hollestelle, A., Minna, J. D., Gazdar, A. F., & Soussi, T.
Affiliations
  • Université Pierre-et-Marie-Curie (FR)
  • The University of Texas Southwestern Medical Center (US)
  • Erasmus MC Cancer Institute (NL)
  • Karolinska Institutet (SE)
Corresponding author
Soussi, Thierry
Venue
Human Mutation
Publisher
Wiley
Published
2014-05-06
Volume
35
Issue
6
Pages
756-765
ISSN
1059-7794 (ISSN-L)
OpenAlex
W2030503662
Cited by
224
References
53
Citations by year
2026: 6 · 2025: 20 · 2024: 16 · 2023: 20 · 2022: 29 · 2021: 25 · 2020: 40 · 2019: 20 · 2018: 18 · 2017: 15 · 2016: 3 · 2015: 7 · 2014: 4
FWCI
1.08
Type
journal article
Language
en
Open access
Locations
  • Human Mutation (publishedVersion)
  • PubMed (publishedVersion)
  • EUR Research Repository (Erasmus University Rotterdam) (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • EUR Research Repository (Erasmus University Rotterdam) (submittedVersion)
Funders
National Cancer Institute (P50CA070907)
Topics
  • Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genomics and Rare Diseases
Keywords
TP53 mutation status, cancer cell lines, cell line misidentification, p53, anticancer drug screening
MeSH terms
Humans, Mutation, Neoplasms, Software, Tumor Suppressor Protein p53, Internet, Databases, Genetic, Cell Line, Tumor

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