Ki-67: more than a proliferation marker

Sun, Xiaoming; Kaufman, Paul D.

doi:10.1007/s00412-018-0659-8

Cited by 963Microtubule and mitosis dynamicsGenomics and Chromatin DynamicsNuclear Structure and Function

Ki-67: more than a proliferation marker

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Sun, Xiaoming, Kaufman, Paul D.·2018·Chromosoma

DOI 10.1007/s00412-018-0659-8PMC PMC5945335

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Abstract

Ki-67 protein has been widely used as a proliferation marker for human tumor cells for decades. In recent studies, multiple molecular functions of this large protein have become better understood. Ki-67 has roles in both interphase and mitotic cells, and its cellular distribution dramatically changes during cell cycle progression. These localizations correlate with distinct functions. For example, during interphase, Ki-67 is required for normal cellular distribution of heterochromatin antigens and for the nucleolar association of heterochromatin. During mitosis, Ki-67 is essential for formation of the perichromosomal layer (PCL), a ribonucleoprotein sheath coating the condensed chromosomes. In this structure, Ki-67 acts to prevent aggregation of mitotic chromosomes. Here, we present an overview of functional roles of Ki-67 across the cell cycle and also describe recent experiments that clarify its role in regulating cell cycle progression in human cells.

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SASP inducers are diverse, but in most cases they depend on NF-κB, CEBPβ, and/or STAT signaling.
“SASP inducers are diverse; however, in most cases, they rely on the involvement of NF-κB, CEBPβ, and/or STAT signaling28.”
From Guidelines for minimal information on cellular senescence experimentation in vivo (2024) · cites this paper as [28]
The Introduction and section 3 discuss Ki-67 in cell-cycle regulation and mention senescent cells, but neither addresses SASP induction. Searches for SASP and for NF-κB, CEBPβ, and STAT signaling found no passage in our copy supporting the attributed claim. (LLM, conf=0.76)
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Authors: Sun, X., & Kaufman, P. D.
Affiliations: University of Massachusetts Chan Medical School (US)
Corresponding author: Kaufman, Paul D.
ORCID: Kaufman, Paul D.
Venue: Chromosoma
Publisher: Springer Science and Business Media LLC
Published: 2018-01-10
Volume: 127
Issue: 2
Pages: 175-186
ISSN: 0009-5915 (ISSN-L)
1432-0886
DOI: 10.1007/s00412-018-0659-8
PMC: PMC5945335
OpenAlex: W2783197533
Cited by: 963
References: 109
Citations by year: 2026: 100 · 2025: 141 · 2024: 162 · 2023: 158 · 2022: 175 · 2021: 122 · 2020: 69 · 2019: 26 · 2018: 8 · 2012: 1
FWCI: 6.31
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: Chromosoma (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Funders: National Institute on Drug Abuse (U01DA040588)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords: Ki-67, cell proliferation markers, cell cycle regulation, heterochromatin, nucleolus, mitosis, interphase, chromosome condensation, ribonucleoprotein
MeSH terms: Protein Domains, Amino Acid Sequence, Cell Nucleolus, Gene Expression Regulation, Heterochromatin, Humans, Interphase, Mitosis, Ribonucleoproteins, Tumor Suppressor Protein p53, Retinoblastoma Protein, Ki-67 Antigen, Protein Isoforms, Cell Line, Tumor, Cell Proliferation, Cyclin-Dependent Kinase Inhibitor p21

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Ki-67: more than a proliferation marker

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