The DOI matches its Crossref record; 47 of the 53 references it cites were found in a registry or an index.
Analysis of TP53 Mutation Status in Human Cancer Cell Lines: A Reassessment
Open accessAbstract
Tumor-derived cell lines play an important role in the investigation of tumor biology and genetics. Across a wide array of studies, they have been tools of choice for the discovery of important genes involved in cancer and for the analysis of the cellular pathways that are impaired by diverse oncogenic events. They are also invaluable for screening novel anticancer drugs. The TP53 protein is a major component of multiple pathways that regulate cellular response to various types of stress. Therefore, TP53 status affects the phenotype of tumor cell lines profoundly and must be carefully ascertained for any experimental project. In the present review, we use the 2014 release of the UMD TP53 database to show that TP53 status is still controversial for numerous cell lines, including some widely used lines from the NCI-60 panel. Our analysis clearly confirms that, despite numerous warnings, the misidentification of cell lines is still present as a silent and neglected issue, and that extreme care must be taken when determining the status of p53, because errors may lead to disastrous experimental interpretations. A novel compendium gathering the TP53 status of 2,500 cell lines has been made available (http://p53.fr). A stand-alone application can be used to browse the database and extract pertinent information on cell lines and associated TP53 mutations. It will be updated regularly to minimize any scientific issues associated with the use of misidentified cell lines (http://p53.fr).
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“In addition, p53 is mutated or altered in multiple immortalized cell lines 62,63 , resulting in altered DDR.”
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The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
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Mutational heterogeneity in cancer and the search for new cancer-associated genes
Lawrence, M. S., Stojanov, P., Polak, P., Kryukov, G. V., Cibulskis, K., Sivachenko, A., Carter, S. L., Stewart, C., Mermel, C. H., Roberts, S. A., et al. (2013) · Nature · doi:10.1038/nature12213 · Cited by 5,461
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A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes
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Self-citationThe NCI60 human tumour cell line anticancer drug screen
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COSMIC: mining complete cancer genomes in the Catalogue of Somatic Mutations in Cancer
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MDM2, MDMX and p53 in oncogenesis and cancer therapy
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Self-citationp53 mutations in colorectal cancer.
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Metadata
- Authors
- Leroy, B., Girard, L., Hollestelle, A., Minna, J. D., Gazdar, A. F., & Soussi, T.
- Affiliations
- Université Pierre-et-Marie-Curie (FR)
- The University of Texas Southwestern Medical Center (US)
- Erasmus MC Cancer Institute (NL)
- Karolinska Institutet (SE)
- Corresponding author
- Soussi, Thierry
- Venue
- Human Mutation
- Publisher
- Wiley
- Published
- 2014-05-06
- Volume
- 35
- Issue
- 6
- Pages
- 756-765
- ISSN
- 1059-7794 (ISSN-L)
- PMC
- PMC4451114
- OpenAlex
- W2030503662
- Cited by
- 224
- References
- 53
- Citations by year
- 2026: 6 · 2025: 20 · 2024: 16 · 2023: 20 · 2022: 29 · 2021: 25 · 2020: 40 · 2019: 20 · 2018: 18 · 2017: 15 · 2016: 3 · 2015: 7 · 2014: 4
- FWCI
- 1.08
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Human Mutation (publishedVersion)
- PubMed (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion)
- Funders
- National Cancer Institute (P50CA070907)
- Topics
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genomics and Rare Diseases
- Keywords
- TP53 mutation status, cancer cell lines, cell line misidentification, p53, anticancer drug screening
- MeSH terms
- Humans, Mutation, Neoplasms, Software, Tumor Suppressor Protein p53, Internet, Databases, Genetic, Cell Line, Tumor
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