Use of a Novel 3D Culture Model to Elucidate the Role of Mesothelial Cells, Fibroblasts and Extra-cellular Matrices on Adhesion and Invasion of Ovarian Cancer Cells to the Omentum
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The omentum is a major site of ovarian cancer metastasis. Our goal was to establish a three-dimensional (3D) model of the key components of the omental microenvironment (mesothelial cells, fibroblasts and extracellular matrices) to study ovarian cancer cell adhesion and invasion. The 3D model comprised of primary human fibroblasts extracted from normal human omentum, mixed with ECM and covered by a layer of primary human mesothelial cells, also from normal human omentum. After addition of ovarian cancer cells, the histological appearance of the 3D culture mimicked microscopic metastases to the omentum from patients with ovarian cancer. When ovarian cancer cells, SKOV3ip.1 and HeyA8, were applied to the 3D omental culture, 60% and 68% of all cells attached, respectively, but only 18% and 25% were able to invade. Ovarian cancer cells preferentially adhered to and invaded collagen I, followed by binding to collagen IV, fibronectin, vitronectin, laminin 10 and 1. Omental mesothelial cells significantly inhibited ovarian cancer cell adhesion and invasion, while omental fibroblasts induced adhesion and invasion. This effect is clearly mediated by direct cell-cell contact, since conditioned medium from mesothelial cells or fibroblasts has a minimal, or no, effect on ovarian cancer cell adhesion and invasion. In summary, we have established a 3D model to study the early steps of ovarian cancer metastasis to the human omentum, and found that omental mesothelial cells inhibit, while omental fibroblasts and the ECM enhance, the attachment and invasion of ovarian cancer cells.
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Three-dimensional organotypic models of the human mesothelium have been utilized for the study of early metastasis to the omentum and interactions with the tumor microenvironment.
“Three-dimensional organotypic models of the human mesothelium (Figure 3) have been utilized for the study of early metastasis to the omentum and interactions with the tumor microenvironment (Kenny et al., 2007).”
From Three-dimensional modelling of ovarian cancer: from cell lines to organoids for discovery and personalized medicine (2022) · cites this paper as Kenny et al., 2007
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Cites
Kalluri, R., & Zeisberg, M. (2006) · Nature Reviews Cancer · doi:10.1038/nrc1877 · Cited by 4,538 · see citing works
Orimo, A., Gupta, P. B., Sgroi, D. C., Arenzana-Seisdedos, F., Delaunay, T., Naeem, R., Carey, V. J., Richardson, A. L., & Weinberg, R. A. (2005) · Cell · doi:10.1016/j.cell.2005.02.034 · Cited by 3,759 · see citing works
Stromal fibroblasts in cancer initiation and progression
Bhowmick, N. A., Neilson, E. G., & Moses, H. L. (2004) · Nature · doi:10.1038/nature03096 · Cited by 2,291 · see citing works
A rapid in vitro assay for quantitating the invasive potential of tumor cells.
Albini, A., Iwamoto, Y., Hk, K., Gr, M., Aaronson, S. A., Jm, K., & McEwan, R. N. (1987) · PubMed · Cited by 1,769 · see citing works
Friends or foes — bipolar effects of the tumour stroma in cancer
Mueller, M. M., & Fusenig, N. E. (2004) · Nature reviews. Cancer · doi:10.1038/nrc1477 · Cited by 1,757 · see citing works
Reactivity of a monoclonal antibody with human ovarian carcinoma.
Bast, R. C., Feeney, M., Lazarus, H., Nadler, L. M., Colvin, R. B., & KNAPP, R. C. (1981) · Journal of Clinical Investigation · doi:10.1172/jci110380 · Cited by 1,661 · see citing works
Ovarian Surface Epithelium: Biology, Endocrinology, and Pathology
Auersperg, N., Wong, A. S. T., Choi, K., Kang, S. K., & Leung, P. C. K. (2001) · Endocrine Reviews · doi:10.1210/edrv.22.2.0422 · Cited by 1,040 · see citing works
Theory and Practice of Histological Techniques
MacSween, R. N. M. (1977) · Journal of Clinical Pathology · doi:10.1136/jcp.30.11.1089-a · Cited by 1,035 · see citing works
Cannistra, S. (1993) · New England Journal of Medicine · doi:10.1056/nejmra041842 · Cited by 957 · see citing works
Debnath, J., Mills, K. R., Collins, N. L., Reginato, M. J., Muthuswamy, S. K., & Brugge, J. S. (2002) · Cell · doi:10.1016/s0092-8674(02)01001-2 · Cited by 763 · see citing works
Hotary, K. B., Allen, E. D., Brooks, P. C., Datta, N. S., Long, M. W., & Weiss, S. J. (2003) · Cell · doi:10.1016/s0092-8674(03)00513-0 · Cited by 677 · see citing works
Wu, Y.-J., Parker, L. M., Binder, N., Beckett, M. A., Sinard, J. H., Griffiths, C. T., & Rheinwald, J. G. (1982) · Cell · doi:10.1016/0092-8674(82)90324-5 · Cited by 513 · see citing works
Ovarian Surface Epithelium: Biology, Endocrinology, and Pathology
Auersperg, N. (2001) · Endocrine Reviews · doi:10.1210/er.22.2.255 · Cited by 439 · see citing works
Mesothelial cells: Their structure, function and role in serosal repair
Mutsaers, S. E. (2002) · Respirology · doi:10.1046/j.1440-1843.2002.00404.x · Cited by 436 · see citing works
Iyengar, P., Combs, T. P., Shah, S. J., Gouon–Evans, V., Pollard, J. W., Albanese, C., Flanagan, L., Tenniswood, M., Guha, C., Lisanti, M. P., et al. (2003) · Oncogene · doi:10.1038/sj.onc.1206737 · Cited by 364 · see citing works
Responses of fibroblasts to anchorage of dorsal extracellular matrix receptors
Beningo, K. A., Dembo, M., & Wang, Y.-L. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0405747102 · Cited by 269 · see citing works
Sawada, K., Radjabi, A. R., Shinomiya, N., Kistner, E. O., Kenny, H. A., Becker, A. R., Turkyilmaz, M. A., Salgia, R., Yamada, S. D., Woude, G. F. V., et al. (2007) · Cancer Research · doi:10.1158/0008-5472.can-06-1147 · Cited by 258 · see citing works
E-cadherin induces mesenchymal-to-epithelial transition in human ovarian surface epithelium
Auersperg, N., Pan, J., Grove, B. D., Peterson, T., Fisher, J., Maines‐Bandiera, S., Somasiri, A. M., & Roskelley, C. D. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.11.6249 · Cited by 254 · see citing works
CD44 and β1 Integrin Mediate Ovarian Carcinoma Cell Adhesion to Peritoneal Mesothelial Cells
Lessan, K., Aguiar, D. J., Oegema, T. R., Siebenson, L., & Skubitz, A. P. N. (1999) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)65406-5 · Cited by 226 · see citing works
β1-Integrins Partly Mediate Binding of Ovarian Cancer Cells to Peritoneal Mesothelium in Vitro
Ströbel, T., & Cannistra, S. A. (1999) · Gynecologic Oncology · doi:10.1006/gyno.1999.5388 · Cited by 168 · see citing works
Topley, N., Brown, Z., Jörres, A., Westwick, J. K., Davies, M., Coles, G. A., & Williams, J. D. (1993) · PubMed Central · Cited by 147 · see citing works
Interactions of human ovarian tumor cells with human mesothelial cells grown on extracellular matrix
Niedbala, M. J., Crickard, K., & Bernacki, R. J. (1985) · Experimental Cell Research · doi:10.1016/0014-4827(85)90197-1 · Cited by 141 · see citing works
Ahmed, N., Riley, C. B., Rice, G. E., & Quinn, M. A. (2005) · Clinical & Experimental Metastasis · doi:10.1007/s10585-005-1262-y · Cited by 140 · see citing works
Jones, M. L., Bancroft, J. D., & Gamble, M. (2008) · Elsevier eBooks · doi:10.1016/b978-0-443-10279-0.50017-8 · Cited by 140 · see citing works
Expression and Function of β1 and αvβ3 Integrins in Ovarian Cancer
Cannistra, S. A., Ottensmeier, C. H. H., Niloff, J. M., Orta, B., & DiCarlo, J. (1995) · Gynecologic Oncology · doi:10.1006/gyno.1995.1214 · Cited by 126 · see citing works
Ströbel, T., Swanson, L., & Cannistra, S. A. (1997) · PubMed · Cited by 123 · see citing works
Guo, W., Lonardo, F., Ueda, T., Kim, T. K., Huvos, A. G., Healey, J. H., & Ladanyi, M. (1999) · International Journal of Cancer · doi:10.1002/(sici)1097-0215(19990118)80:2 · Cited by 120 · see citing works
Expression and function of β1 and αvβ3 integrins in ovarian cancer
(1996) · International Journal of Gynecology & Obstetrics · doi:10.1016/0020-7292(96)84550-4 · Cited by 120 · see citing works
A comparative study of the structure of human and murine greater omentum
Wilkosz, S., Ireland, G. W., Khwaja, N., Walker, M. G., Butt, R. P., Giorgio-Miller, A. D., & Herrick, S. E. (2005) · Anatomy and Embryology · doi:10.1007/s00429-004-0446-6 · Cited by 110 · see citing works
Calcitonin receptors, bone sialoprotein and osteopontin are expressed in primary breast cancers
Gillespie, M. T., Thomas, R. J., Pu, Z., Zhou, H., Martin, T. J., & Findlay, D. M. (1997) · International Journal of Cancer · doi:10.1002/(sici)1097-0215(19971210)73:6 · Cited by 99 · see citing works
Composition of the extracellular matrix of the peritoneum
Witz, C. (2001) · Journal of the Society for Gynecologic Investigation · doi:10.1016/s1071-5576(01)00122-8 · Cited by 96 · see citing works
Ried, S., Jäger, C., Jeffers, M., Woude, G. F. V., Graeff, H., Schmitt, M., & Lengyel, E. R. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.23.16377 · Cited by 88 · see citing works
Sengupta, S., Xiao, Y., & Xu, Y. (2003) · The FASEB Journal · doi:10.1096/fj.02-1145fje · Cited by 83 · see citing works
Himelstein, B. P., Cañete-Soler, R., Bernhard, E. J., & Muschel, R. J. (1994) · Journal of Cell Science · doi:10.1242/jcs.107.2.477 · Cited by 81 · see citing works
Lengyel, E. R., Gum, R. J., Juárez, J., Clayman, G. L., Seiki, M., Sato, H., & Boyd, D. D. (1995) · PubMed · Cited by 78 · see citing works
Tumor Growth and Dissemination After Laparotomy and CO2 Pneumoperitoneum: A Rat Ovarian Cancer Model
Canis, M. J. (1998) · Obstetrics and Gynecology · doi:10.1016/s0029-7844(98)00145-8 · Cited by 68 · see citing works
Diet and other risk factors for cancer of the salivary glands: A population-based case-control study
Zheng, W., Shu, X., Ji, B., & Gao, Y. (1996) · International Journal of Cancer · doi:10.1002/(sici)1097-0215(19960717)67:2 · Cited by 59 · see citing works
MMP-2 release and activation in ovarian carcinoma: the role of fibroblasts
Boyd, R. S., & Balkwill, F. R. (1999) · British Journal of Cancer · doi:10.1038/sj.bjc.6690357 · Cited by 58 · see citing works
Pathology of the peritoneum: a review of selected topics.
Daya, D., & McCaughey, W. (1991) · PubMed · Cited by 57 · see citing works
Establishment and functional characterization of human peritoneal fibroblasts in culture
Jörres, A., Ludat, K., Lang, J., Sander, K., Gahl, G. M., Frei, U. A., DeJonge, K., Williams, J. D., & Topley, N. (1996) · Journal of the American Society of Nephrology · doi:10.1681/asn.v7102192 · Cited by 53 · see citing works
Zhu, G., Risteli, J., Puistola, U., Kauppila, A., & Risteli, L. (1993) · PubMed · Cited by 52 · see citing works
Turck, N., Groß, I., Gendry, P., Stutzmann, J., Freund, J., Kédinger, M., Simon-Assmann, P. M., & Launay, J. (2004) · Experimental Cell Research · doi:10.1016/j.yexcr.2004.10.025 · Cited by 51 · see citing works
Hepatocyte Growth Factor/Scatter Factor Released during Peritonitis Is Active on Mesothelial Cells
Rampino, T., Cancarini, G. C., Gregorini, M., Guallini, P., Maggio, M., Ranghino, A., Soccio, G., & Canton, A. D. (2001) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)62514-x · Cited by 49 · see citing works
Zhang, X. (1999) · Journal of the Society for Gynecologic Investigation · doi:10.1016/s1071-5576(99)00040-4 · Cited by 48 · see citing works
Primary culture and mRNA analysis of human ovarian cells
Dunfield, L., Shepherd, T. G., & Nachtigal, M. W. (2002) · Biological Procedures Online · doi:10.1251/bpo34 · Cited by 42 · see citing works
Neoangiogenesis: A putative marker of malignancy in non-small-cell-lung-cancer (NSCLC) development
Fontanini, G., Vignati, S., Bigini, D., Lucchi, M., Mussi, A., Basolo, F., Angeletti, C. A., & Bevilacqua, G. (1996) · International Journal of Cancer · doi:10.1002/(sici)1097-0215(19960904)67:5 · Cited by 34 · see citing works
Integrity of the 1,25-dihydroxyvitamin D3 receptor in bone, lung, and other cancers
Miller, C. W., Roberta Morosetti, A., Campbell, M. J., Mendoza, S., & Koeffler, H. P. (1997) · Molecular Carcinogenesis · doi:10.1002/(sici)1098-2744(199708)19:4 · Cited by 33 · see citing works
Sampling the omentum in ovarian neoplasia: when one block is enough
Doig, T., & Monaghan, H. (2006) · International Journal of Gynecological Cancer · doi:10.1111/j.1525-1438.2006.00273.x · Cited by 31 · see citing works
Westerlund, A., Hujanen, E., Puistola, U., & Turpeenniemi-Hujanen, T. (1997) · Gynecologic Oncology · doi:10.1006/gyno.1997.4808 · Cited by 30 · see citing works
Zhang, X., Pettengell, R., Nasiri, N., et al. (1999) · Journal of the Society for Gynecologic Investigation · doi:10.1177/107155769900600610 · Cited by 29 · see citing works
Casey, R. C., Koch, K. A., Oegema, T. R., Skubitz, K. M., Pambuccian, S. E., Grindle, S. M., & Skubitz, A. P. N. (2003) · Clinical & Experimental Metastasis · doi:10.1023/a:1024009131191 · Cited by 28 · see citing works
An experimental model for ovarian tumor invasion of cultured mesothelial cell monolayer.
Sawada, M., Shii, J., Akedo, H., & Tanizawa, O. (1994) · PubMed · Cited by 28 · see citing works
Composition of the Extracellular Matrix of the Peritoneum
Witz, C. A., Montoya-Rodriguez, I. A., Cho, S., Centonze, V. E., Bonewald, L. F., & Schenken, R. S. (2001) · Journal of the Society for Gynecologic Investigation · doi:10.1177/107155760100800508 · Cited by 24 · see citing works
The Variation in Outline of American “Flint” Arrow-heads
Abbott, C. C. (1872) · Nature · doi:10.1038/006392b0 · Cited by 8 · see citing works
Flagged first, then by citations
Research works printed but not linked: 6
- Tavassoli FA, Devilee P. Tumors of the ovary and peritoneum. In: Kleihues P, Sobin L, eds. Tumors of the breast and genital organs. Lyon, France: IARC Press, 2003.113-203.
- Rieppi M, Vergani V, Gatto C, Zanetta G, Allavena P, Taraboletti G, Giavazzi R. Mesothelial cells induce the motility of human ovarian carcinoma cells. Int J Cancer 1999;80:303-7.
- Moser TL, Pizzo SV, Bafetti L, Fishman DA, Stack MS. Evidence for preferential adhesion of ovarian epithelial carcinoma cells to type I col- lagen mediated by the a2b1 integrin. Int J Cancer 1996;67:695-701.
- Yashiro M, Chung Y, Inoue T, Nishimura S, Matsuoka T, Fujihara T, Sowa M. Hepatocyte growth factor (HGF) produced by peritoneal fibroblasts may affect mesothelial cell morphology and promote peri- toneal dissemination. Int J Cancer 1996;67:289-93.
- Borchers AH, Steinbauer H, Schafer BS, Kramer M, Bowden GT, Fusenig N. Fibroblast-directed expression and localization of 92-kDa type IV collagenase along the tumor-stroma interface in an in vitro three-dimensional model of human squamous cell carcinoma. Mol Carcinog 1997;19:258-66.
- van der Wal BCH, Hofland LJ, Marquet RL, van Koetsveld PM, van Rossen MEE, van Eijck CHJ. Paracrine interactions between meso- thelial and colon-carcinoma cells in a rat model. Int J Cancer 1997;73:885-90.
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Lengyel, E. R. (2010) · American Journal Of Pathology · doi:10.2353/ajpath.2010.100105 · Cited by 1,679 · see citing works
Cancer-Associated Stromal Fibroblasts Promote Pancreatic Tumor Progression
Hwang, R. F., Moore, T. T., Arumugam, T., Ramachandran, V., Amos, K. D., Rivera, A. M., Ji, B., Evans, D. B., & Logsdon, C. D. (2008) · Cancer Research · doi:10.1158/0008-5472.can-07-5714 · Cited by 1,093 · see citing works
Loessner, D., Meinert, C., Kaemmerer, E., Martine, L. C., Yue, K., Levett, P. A., Klein, T. J., Melchels, F. P. W., Khademhosseini, A., & Hutmacher, D. W. (2016) · Nature Protocols · doi:10.1038/nprot.2016.037 · Cited by 868 · see citing works
High-Grade Serous Ovarian Cancer: Basic Sciences, Clinical and Therapeutic Standpoints
Lisio, M. A., Fu, L., Goyeneche, A. A., Gao, Z., & Telleria, C. M. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20040952 · Cited by 685 · see citing works
Loessner, D., Stok, K. S., Lutolf, M. P., Hutmacher, D. W., Clements, J. A., & Rizzi, S. C. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.07.064 · Cited by 602 · see citing works
Gjerdrum, C., Tiron, C., Høiby, T., Stefansson, I., Haugen, H., Sandal, T., Collett, K., Li, S., McCormack, E., Gjertsen, B. T., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0909333107 · Cited by 594 · see citing works
Screening out irrelevant cell-based models of disease
Horvath, P., Aulner, N., Bickle, M., Davies, A. M., Nery, E. D., Ebner, D., Montoya, M. C., Östling, P., Pietiäinen, V., Price, L. S., et al. (2016) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2016.175 · Cited by 525 · see citing works
Adipocyte-induced CD36 expression drives ovarian cancer progression and metastasis
Ladányi, A., Mukherjee, A., Kenny, H. A., Johnson, A., Mitra, A. K., Sundaresan, S., Nieman, K. M., Pascual, G., Benitah, S. A., MONTAG, A. G., et al. (2018) · Oncogene · doi:10.1038/s41388-017-0093-z · Cited by 522 · see citing works
Neutrophils facilitate ovarian cancer premetastatic niche formation in the omentum
Lee, W., Ko, S. Y., Mohamed, M. S., Kenny, H. A., Lengyel, E., & Naora, H. (2019) · Journal of Experimental Medicine · doi:10.1084/jem.20181170 · Cited by 478 · see citing works
Liu, X., & Locasale, J. W. (2017) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2017.01.004 · Cited by 453 · see citing works
Yeung, T.-L., Leung, C. S., Yip, K., Yeung, C. L. A., Wong, S. T. C., & Mok, S. C. (2015) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00188.2015 · Cited by 379 · see citing works
MicroRNAs Reprogram Normal Fibroblasts into Cancer-Associated Fibroblasts in Ovarian Cancer
Mitra, A. K., Zillhardt, M., Hua, Y., Tiwari, P., Murmann, A. E., Peter, M. E., & Lengyel, E. R. (2012) · Cancer Discovery · doi:10.1158/2159-8290.cd-12-0206 · Cited by 371 · see citing works
Kenny, H. A., Kaur, S., Coussens, L. M., & Lengyel, E. R. (2008) · Journal of Clinical Investigation · doi:10.1172/jci33775 · Cited by 370 · see citing works
Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis
Cui, C., Chakraborty, K., Tang, X. A., Zhou, G., Schoenfelt, K. Q., Becker, K. M., Hoffman, A., Chang, Y.-F., Blank, A., Reardon, C. A., et al. (2021) · Cell · doi:10.1016/j.cell.2021.04.016 · Cited by 366 · see citing works
Loss of E-Cadherin Promotes Ovarian Cancer Metastasis via α5-Integrin, which Is a Therapeutic Target
Sawada, K., Mitra, A. K., Radjabi, A. R., Bhaskar, V., Kistner, E. O., Tretiakova, M., Jagadeeswaran, S., MONTAG, A. G., Becker, A. R., Kenny, H. A., et al. (2008) · Cancer Research · doi:10.1158/0008-5472.can-07-5167 · Cited by 356 · see citing works
Xu, F., Celli, J., Rizvi, I., Moon, S., Hasan, T., & Demirci, U. (2011) · Biotechnology Journal · doi:10.1002/biot.201000340 · Cited by 337 · see citing works
Mesothelial Cells Promote Early Ovarian Cancer Metastasis through Fibronectin Secretion
Kenny, H. A., Chiang, C.-Y., White, E. A., Schryver, E. M., Habis, M., Romero, I. L., Ladanyi, A., Penicka, C. V., George, J., Matlin, K., et al. (2014) · Journal of Clinical Investigation · doi:10.1172/jci74778 · Cited by 303 · see citing works
Lee, J. M., Mhawech-Fauceglia, P. Y., Lee, N., Parsanian, L. C., Lin, Y. G., Gayther, S. A., & Lawrenson, K. (2013) · Laboratory Investigation · doi:10.1038/labinvest.2013.41 · Cited by 302 · see citing works
Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis
Curtis, M., Kenny, H. A., Ashcroft, B., Mukherjee, A., Johnson, A., Zhang, Y., Helou, Y. A., Batlle, R., Liu, X., Gutiérrez, N. M., et al. (2018) · Cell Metabolism · doi:10.1016/j.cmet.2018.08.007 · Cited by 292 · see citing works
Rizvi, I., Gurkan, U. A., Tasoglu, S., Alagic, N., Celli, J. P., Mensah, L. B., Mai, Z., Demirci, U., & Hasan, T. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1216989110 · Cited by 234 · see citing works
Can tissue engineering concepts advance tumor biology research?
Hutmacher, D. W., Loessner, D., Rizzi, S. C., Kaplan, D. L., Mooney, D. J., & Clements, J. A. (2010) · Trends in biotechnology · doi:10.1016/j.tibtech.2009.12.001 · Cited by 237 · see citing works
Nakamura, K., Sawada, K., Kinose, Y., Yoshimura, A., Toda, A., Nakatsuka, E., Hashimoto, K., Mabuchi, S., Morishige, K., Kurachi, H., et al. (2016) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-16-0191 · Cited by 228 · see citing works
Motohara, T., Masuda, K., Morotti, M., Zheng, Y., El-Sahhar, S., Chong, K. Y., Wietek, N., Alsaadi, A., Carrami, E. M., Hu, Z., et al. (2019) · Oncogene · doi:10.1038/s41388-018-0637-x · Cited by 218 · see citing works
Epithelial ovarian cancer experimental models
Lengyel, E. R., Burdette, J. E., Kenny, H. A., Matei, D. E., Pilrose, J. M., Haluska, P., Nephew, K. P., Hales, D. B., & Stack, M. S. (2013) · Oncogene · doi:10.1038/onc.2013.321 · Cited by 209 · see citing works
Mikuła‐Pietrasik, J., Uruski, P., Tykarski, A., & Książek, K. (2017) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-017-2663-1 · Cited by 195 · see citing works
Rizvi, I., Celli, J. P., Evans, C. L., Abu‐Yousif, A. O., Muzikansky, A., Pogue, B. W., Finkelstein, D. M., & Hasan, T. G. (2010) · Cancer Research · doi:10.1158/0008-5472.can-10-1783 · Cited by 191 · see citing works
Fibroblasts in three dimensional matrices: cell migration and matrix remodeling
Rhee, S. (2009) · Experimental & Molecular Medicine · doi:10.3858/emm.2009.41.12.096 · Cited by 185 · see citing works
Sandoval, P., Jiménez-Heffernan, J. A., Rynne‐Vidal, Á., Lozano, M. L. P., Gilsanz, Á., Ruiz‐Carpio, V., Reyes, R., García‐Bordas, J., Stamatakis, K., Dotor, J., et al. (2013) · The Journal of Pathology · doi:10.1002/path.4281 · Cited by 186 · see citing works
Thibault, B., Castells, M., Delord, J., & Couderc, B. C. (2013) · Cancer and Metastasis Reviews · doi:10.1007/s10555-013-9456-2 · Cited by 181 · see citing works
Cancer systems biology: embracing complexity to develop better anticancer therapeutic strategies
Du, W., & Elemento, O. (2014) · Oncogene · doi:10.1038/onc.2014.291 · Cited by 182 · see citing works
Cancer Systems Biology: a peek into the future of patient care?
Werner, H. M. J., Mills, G. B., & Ram, P. T. (2014) · Nature Reviews Clinical Oncology · doi:10.1038/nrclinonc.2014.6 · Cited by 175 · see citing works
Cai, J., Tang, H., Xu, L., Wang, X., Yang, C., Ruan, S., Guo, J., Hu, S., & Wang, Z. (2012) · Carcinogenesis · doi:10.1093/carcin/bgr230 · Cited by 173 · see citing works
Three-dimensional in vitro cancer models: a short review
Wang, C., Tang, Z., Zhao, Y., Yao, R., Li, L., & Sun, W. (2014) · Biofabrication · doi:10.1088/1758-5082/6/2/022001 · Cited by 173 · see citing works
Kenny, H. A., Lal‐Nag, M., White, E. A., Shen, M., Chiang, C.-Y., Mitra, A. K., Zhang, Y., Curtis, M., Schryver, E. M., Bettis, S., et al. (2015) · Nature Communications · doi:10.1038/ncomms7220 · Cited by 172 · see citing works
Cell–cell and cell–matrix dynamics in intraperitoneal cancer metastasis
Sodek, K. L., Murphy, K., Brown, T. J., & Ringuette, M. J. (2012) · Cancer and Metastasis Reviews · doi:10.1007/s10555-012-9351-2 · Cited by 156 · see citing works
Schauer, I. G., Sood, A. K., Mok, S. C., & Liu, J. (2011) · Neoplasia · doi:10.1593/neo.101720 · Cited by 155 · see citing works
Clark, R., Krishnan, V., Schoof, M., Rodriguez, I., Theriault, B. R., Chekmareva, M. A., & Rinker-Schaeffer, C. W. (2013) · American Journal Of Pathology · doi:10.1016/j.ajpath.2013.04.023 · Cited by 153 · see citing works
MMP-2 functions as an early response protein in ovarian cancer metastasis
Kenny, H. A., & Lengyel, E. R. (2009) · Cell Cycle · doi:10.4161/cc.8.5.7703 · Cited by 149 · see citing works
Yoshimura, A., Sawada, K., Nakamura, K., Kinose, Y., Nakatsuka, E., Kobayashi, M., Miyamoto, M., Ishida, K., Matsumoto, Y., Kodama, M., et al. (2018) · BMC Cancer · doi:10.1186/s12885-018-4974-5 · Cited by 145 · see citing works
HOXA9 promotes ovarian cancer growth by stimulating cancer-associated fibroblasts
Ko, S. Y., Barengo, N., Ladanyi, A., Lee, J.-S., Marini, F., Lengyel, E., & Naora, H. (2012) · Journal of Clinical Investigation · doi:10.1172/jci62229 · Cited by 132 · see citing works
Mesenchymal gene program–expressing ovarian cancer spheroids exhibit enhanced mesothelial clearance
Davidowitz, R. A., Selfors, L. M., Iwanicki, M., Elias, K. M., Karst, A. M., Piao, H., Ince, T. A., Drage, M. G., Dering, J., Konecny, G. E., et al. (2014) · Journal of Clinical Investigation · doi:10.1172/jci69815 · Cited by 132 · see citing works
Zillhardt, M., Park, S.-M., Romero, I. L., Sawada, K., MONTAG, A. G., Krausz, T. N., Yamada, S. D., Peter, M. E., & Lengyel, E. R. (2011) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-10-3387 · Cited by 116 · see citing works
Sodek, K. L., Brown, T. J., & Ringuette, M. J. (2008) · BMC Cancer · doi:10.1186/1471-2407-8-223 · Cited by 111 · see citing works
Kobayashi, M., Sawada, K., Miyamoto, M., Shimizu, A., Yamamoto, M., Kinose, Y., Nakamura, K., Kawano, M., Kodama, M., Hashimoto, K., et al. (2020) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2020.04.076 · Cited by 108 · see citing works
Moss, N. M., Barbolina, M. V., Liu, Y., Sun, L., Munshi, H. G., & Stack, M. S. (2009) · Cancer Research · doi:10.1158/0008-5472.can-08-4151 · Cited by 105 · see citing works
The Mesothelial Origin of Carcinoma Associated-Fibroblasts in Peritoneal Metastasis
Rynne‐Vidal, Á., Jiménez-Heffernan, J. A., Fernández‐Chacón, C., López‐Cabrera, M., & Sandoval, P. (2015) · Cancers · doi:10.3390/cancers7040872 · Cited by 104 · see citing works
Drug screening in 3D in vitro tumor models: overcoming current pitfalls of efficacy read‐outs
Santo, V. E., Rebelo, S. P., Estrada, M. F., Alves, P. M., Boghaert, E. R., & Brito, C. (2016) · Biotechnology Journal · doi:10.1002/biot.201600505 · Cited by 103 · see citing works
An integrated analysis identifies STAT4 as a key regulator of ovarian cancer metastasis
Zhao, L., Ji, G., Le, X., Luo, Z., Wang, C., Feng, M., Xu, L., Zhang, Y., Lau, W. B., Lau, B., et al. (2017) · Oncogene · doi:10.1038/onc.2016.487 · Cited by 100 · see citing works
Sun, Y., Fan, X., Zhang, Q. H., Shi, X., Xu, G., & Zou, C. (2017) · Tumor Biology · doi:10.1177/1010428317712592 · Cited by 100 · see citing works
Deying, W., Feng, G., Shumei, L., Zhao, H., Liu, M., & Hongqing, W. (2017) · Bioscience Reports · doi:10.1042/bsr20160470 · Cited by 99 · see citing works
Kenny, H. A., Dogan, S., Zillhardt, M., Mitra, A. K., Yamada, S. D., Krausz, T. N., & Lengyel, E. R. (2009) · Cancer treatment and research · doi:10.1007/978-0-387-98094-2_16 · Cited by 91 · see citing works
Current Status of Patient-Derived Ovarian Cancer Models
Maru, Y., & Hippo, Y. (2019) · Cells · doi:10.3390/cells8050505 · Cited by 88 · see citing works
Usui, A., Ko, S. Y., Barengo, N., & Naora, H. (2014) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-13-0489 · Cited by 85 · see citing works
Ubink, I., van Eden, W. J., Snæbjörnsson, P., Kok, N. F. M., van Kuik, J., van Grevenstein, W. M. U., Laclé, M. M., Sanders, J., Fijneman, R. J. A., Elias, S. G., et al. (2018) · British journal of surgery · doi:10.1002/bjs.10788 · Cited by 80 · see citing works
Metalloproteinases in Ovarian Cancer
Carey, P. M., Low, E. L., Harper, E. I., & Stack, M. S. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22073403 · Cited by 82 · see citing works
The First Line of Intra-abdominal Metastatic Attack: Breaching the Mesothelial Cell Layer
Kenny, H. A., Nieman, K. M., Mitra, A. K., & Lengyel, E. R. (2011) · Cancer Discovery · doi:10.1158/2159-8290.cd-11-0117 · Cited by 79 · see citing works
Therapeutic Targeting of Collective Invasion in Ovarian Cancer
Moffitt, L. R., Karimnia, N., Stephens, A. N., & Bilandzic, M. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20061466 · Cited by 78 · see citing works
Pinto, E. B., Sawazaki‐Calone, Í., Macedo, C. C. S., Carnielli, C. M., de Oliveira, C. E., Rodrigues, P. C., Rangel, A. L. C. A., dos Santos, J. N., Risteli, J., Graner, E., et al. (2016) · Tumor Biology · doi:10.1007/s13277-015-4629-y · Cited by 78 · see citing works
Targeting the Urokinase Plasminogen Activator Receptor Inhibits Ovarian Cancer Metastasis
Kenny, H. A., Leonhardt, P., Ladányi, A., Yamada, S. D., MONTAG, A. G., Im, H. K., Jagadeeswaran, S., Shaw, D. E., Mazar, A. P., & Lengyel, E. R. (2010) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-10-2258 · Cited by 77 · see citing works
Microenvironment-induced downregulation of miR-193b drives ovarian cancer metastasis
Mitra, A. K., Chiang, C. Y., Tiwari, P., Tomar, S., Watters, K. M., Peter, M. E., & Lengyel, E. R. (2015) · Oncogene · doi:10.1038/onc.2015.43 · Cited by 74 · see citing works
A novel asymmetric 3D in-vitro assay for the study of tumor cell invasion
Brekhman, V., & Neufeld, G. (2009) · BMC Cancer · doi:10.1186/1471-2407-9-415 · Cited by 73 · see citing works
Kaur, S., Kenny, H. A., Jagadeeswaran, S., Zillhardt, M., MONTAG, A. G., Kistner, E. O., Yamada, S. D., Mitra, A. K., & Lengyel, E. R. (2009) · American Journal Of Pathology · doi:10.2353/ajpath.2009.090028 · Cited by 72 · see citing works
Carroll, M. J., Fogg, K. C., Patel, H. A., Krause, H. B., Mancha, A.-S., Patankar, M. S., Weisman, P. S., Barroilhet, L. M., & Kreeger, P. K. (2018) · Cancer Research · doi:10.1158/0008-5472.can-17-3341 · Cited by 72 · see citing works
Visualizing the Network of Bonghan Ducts in the Omentum and Peritoneum by Using Trypan Blue
Lee, B.-C., Kim, K. W., & Soh, K. (2009) · Journal of Acupuncture and Meridian Studies · doi:10.1016/s2005-2901(09)60017-0 · Cited by 69 · see citing works
Yee, C., Dickson, K.-A., Muntasir, M. N., Ma, Y., & Marsh, D. J. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.836984 · Cited by 66 · see citing works
Biology-driven therapy advances in high-grade serous ovarian cancer
Wang, Y., Duval, A. J., Adli, M., & Matei, D. E. (2024) · Journal of Clinical Investigation · doi:10.1172/jci174013 · Cited by 70 · see citing works
Adaptive RSK‐EphA2‐GPRC5A signaling switch triggers chemotherapy resistance in ovarian cancer
Moyano‐Galceran, L., Pietilä, E. A., Turunen, S. P., Corvigno, S., Hjerpe, E., Bulanova, D. R., Joneborg, U., Alkasalias, T., Miki, Y., Yashiro, M., et al. (2020) · EMBO Molecular Medicine · doi:10.15252/emmm.201911177 · Cited by 67 · see citing works
Protein Kinase A Activity and Anchoring Are Required for Ovarian Cancer Cell Migration and Invasion
McKenzie, A. J., Campbell, S. L., & Howe, A. K. (2011) · PLoS ONE · doi:10.1371/journal.pone.0026552 · Cited by 66 · see citing works
Gonadotropin signalling in epithelial ovarian cancer
Mertens‐Walker, I., Baxter, R. C., & Marsh, D. J. (2012) · Cancer Letters · doi:10.1016/j.canlet.2012.05.017 · Cited by 63 · see citing works
Engineering a High-Throughput 3-D In Vitro Glioblastoma Model
Fan, Y., Avci, N. G., Nguyen, D. T., Dragomir, A., Akay, Y. M., Xu, F., & Akay, M. (2015) · IEEE Journal of Translational Engineering in Health and Medicine · doi:10.1109/jtehm.2015.2410277 · Cited by 63 · see citing works
Tumor microenvironment–associated modifications of alternative splicing
Brosseau, J., Lucier, J., Nwilati, H., Thibault, P., Garneau, D., Gendron, D., Durand, M., Couture, S., Lapointe, E., Prinos, P., et al. (2013) · RNA · doi:10.1261/rna.042168.113 · Cited by 63 · see citing works
Bilandzic, M., Rainczuk, A., Green, E., Fairweather, N., Jobling, T. W., Plebanski, M., & Stephens, A. N. (2019) · Cancers · doi:10.3390/cancers11091228 · Cited by 63 · see citing works
Laoukili, J., Constantinides, A., Wassenaar, E. C. E., Elias, S. G., Raats, D., van Schelven, S. J., van Wettum, J., Volckmann, R., Köster, J., Huitema, A. D. R., et al. (2022) · British Journal of Cancer · doi:10.1038/s41416-022-01742-5 · Cited by 62 · see citing works
Sulaiman, S. A., Mutalib, N. A., & Jamal, R. A. (2016) · Frontiers in Pharmacology · doi:10.3389/fphar.2016.00271 · Cited by 63 · see citing works
Three‐dimensional in vitro cell biology models of ovarian and endometrial cancer
Grün, B., Benjamin, E. O., Sinclair, J., Timms, J. F., Jacobs, I. J., Gayther, S. A., & Dafou, D. (2009) · Cell Proliferation · doi:10.1111/j.1365-2184.2008.00579.x · Cited by 60 · see citing works
Tissue-engineered human 3D model of bladder cancer for invasion study and drug discovery
Goulet, C. R., Bernard, G., Chabaud, S., Couture, A., Langlois, A., Neveu, B., Pouliot, F., & Bolduc, S. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.08.041 · Cited by 60 · see citing works
A 3D Microfluidic Model to Recapitulate Cancer Cell Migration and Invasion
Toh, Y.-C., Raja, A., Yu, H., & Van Noort, D. (2018) · Bioengineering · doi:10.3390/bioengineering5020029 · Cited by 57 · see citing works
The proto-oncogene c-Fos transcriptionally regulates VEGF production during peritoneal inflammation
Catar, R. A., Witowski, J., Wagner, P., Schramm, I., Kawka, E., Philippe, A., Dragun, D., & Jörres, A. (2013) · Kidney International · doi:10.1038/ki.2013.217 · Cited by 59 · see citing works
Panagopoulos, V., Leach, D. A., Zinonos, I., Ponomarev, V., Licari, G., Liapis, V., Ingman, W. V., Anderson, P., DeNichilo, M. O., & Evdokiou, A. (2017) · International Journal of Oncology · doi:10.3892/ijo.2017.3883 · Cited by 58 · see citing works
Loessner, D., Holzapfel, B. M., & Clements, J. A. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.06.001 · Cited by 57 · see citing works
A Novel Intravital Imaging Window for Longitudinal Microscopy of the Mouse Ovary
Bochner, F., Fellus-Alyagor, L., Kalchenko, V., Shinar, S., & Neeman, M. (2015) · Scientific Reports · doi:10.1038/srep12446 · Cited by 57 · see citing works
An omentum-inspired 3d peg hydrogel for identifying ecm-drivers of drug resistant ovarian cancer
Brooks, E. A., Gencoglu, M. F., Corbett, D. C., Stevens, K. R., & Peyton, S. R. (2019) · APL Bioengineering · doi:10.1063/1.5091713 · Cited by 51 · see citing works
Roy, V., Magne, B., Vaillancourt-Audet, M., Blais, M., Chabaud, S., Grammond, E., Piquet, L., Fradette, J., Laverdière, I., Moulin, V. J., et al. (2020) · BioMed Research International · doi:10.1155/2020/6051210 · Cited by 53 · see citing works
Mutant p53 regulates ovarian cancer transformed phenotypes through autocrine matrix deposition
Iwanicki, M., Chen, H., Iavarone, C., Zervantonakis, I. K., Muranen, T. E., Novak, M., Ince, T. A., Drapkin, R., & Brugge, J. S. (2016) · JCI Insight · doi:10.1172/jci.insight.86829 · Cited by 54 · see citing works
Ibrahim, L. I., Hajal, C., Offeddu, G. S., Gillrie, M. R., & Kamm, R. D. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121728 · Cited by 53 · see citing works
In vitro metastatic colonization of human ovarian cancer cells to the omentum
Khan, S., Funk, H. M., Thiolloy, S., Lotan, T. L., Hickson, J. A., Prins, G. S., Drew, A. F., & Rinker-Schaeffer, C. W. (2010) · Clinical & Experimental Metastasis · doi:10.1007/s10585-010-9317-0 · Cited by 53 · see citing works
Microenvironmental Regulation of Ovarian Cancer Metastasis
Barbolina, M. V., Moss, N. M., Westfall, S. D., Liu, Y., Burkhalter, R. J., Marga, F. S., Forgács, G., Hudson, L. G., & Stack, M. S. (2009) · Cancer treatment and research · doi:10.1007/978-0-387-98094-2_15 · Cited by 53 · see citing works
Akinjiyan, F. A., Ibitoye, Z., Zhao, P., Shriver, L. P., Patti, G. J., Longmore, G. D., & Fuh, K. C. (2023) · Oncogene · doi:10.1038/s41388-023-02884-3 · Cited by 50 · see citing works
Cancer-associated fibroblasts as target and tool in cancer therapeutics and diagnostics
Vlieghere, E. D., Verset, L., Demetter, P., Bracke, M., & de Wever, O. (2015) · Virchows Archiv · doi:10.1007/s00428-015-1818-4 · Cited by 50 · see citing works
Organotypic 3D Models of the Ovarian Cancer Tumor Microenvironment
Watters, K. M., Bajwa, P., & Kenny, H. A. (2018) · Cancers · doi:10.3390/cancers10080265 · Cited by 49 · see citing works
Bajwa, P., Kordylewicz, K., Bilecz, A. J., Lastra, R. R., Wroblewski, K., Rinkevich, Y., Lengyel, E. R., & Kenny, H. A. (2023) · JCI Insight · doi:10.1172/jci.insight.163019 · Cited by 49 · see citing works
Mesenchymal Cell Interaction with Ovarian Cancer Cells Triggers Pro-Metastatic Properties
Lis, R., Touboul, C., Raynaud, C. M., Malek, J. A., Suhre, K., Mirshahi, M., & Rafii, A. (2012) · PLoS ONE · doi:10.1371/journal.pone.0038340 · Cited by 47 · see citing works
Jones, P., & Drapkin, R. (2013) · Frontiers in Oncology · doi:10.3389/fonc.2013.00217 · Cited by 46 · see citing works
Bockstal, M. V., Lambein, K., Gele, M. V., Vlieghere, E. D., Limame, R., Braems, G., den Broecke, R. V., Cocquyt, V., Denys, H. G., Bracke, M., et al. (2014) · Oncoscience · doi:10.18632/oncoscience.87 · Cited by 46 · see citing works
Asem, M., Young, A., Oyama, C., Zerda, A. C. D. L., Liu, Y., Ravosa, M. J., Gupta, V. G., Jewell, A., Khabele, D., & Stack, M. S. (2020) · Scientific Reports · doi:10.1038/s41598-020-68639-2 · Cited by 46 · see citing works
Life is Three Dimensional—As In Vitro Cancer Cultures Should Be
Levinger, I., Ventura, Y., & Vago, R. (2014) · Advances in cancer research · doi:10.1016/b978-0-12-800249-0.00009-3 · Cited by 45 · see citing works
Harper, E. I., Sheedy, E. F., & Stack, M. S. (2018) · Cancers · doi:10.3390/cancers10070230 · Cited by 45 · see citing works
In vitro Mesothelial Clearance Assay that Models the Early Steps of Ovarian Cancer Metastasis
Davidowitz, R. A., Iwanicki, M., & Brugge, J. S. (2012) · Journal of Visualized Experiments · doi:10.3791/3888 · Cited by 44 · see citing works
Three-dimensional modeling of ovarian cancer
White, E. A., Kenny, H. A., & Lengyel, E. R. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.07.003 · Cited by 42 · see citing works
Systems microscopy approaches to understand cancer cell migration and metastasis
Dévédec, S. E. L., Yan, K., de Bont, H., Ghotra, V. P. S., Truong, H. H., Danen, E. H. J., Verbeek, F. J., & van de Water, B. (2010) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-010-0419-2 · Cited by 41 · see citing works
- Authors
- Kenny, H. A., Krausz, T., Yamada, S. D., & Lengyel, E.
- Affiliations
- University of Chicago (US)
- Corresponding author
- Lengyel, Ernst
- Venue
- International Journal of Cancer
- Publisher
- Wiley
- Published
- 2007-06
- Volume
- 121
- Issue
- 7
- Pages
- 1463-1472
- ISSN
- 0020-7136 (ISSN-L)
- 1097-0215
- OpenAlex
- W2040167467
- Cited by
- 243
- References
- 52
- Citations by year
- 2026: 2 · 2025: 12 · 2024: 10 · 2023: 14 · 2022: 20 · 2021: 17 · 2020: 10 · 2019: 16 · 2018: 13 · 2017: 14 · 2016: 16 · 2015: 15 · 2014: 20 · 2013: 15 · 2012: 10
- FWCI
- 10.64
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- International Journal of Cancer (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ovarian Cancer Research Fund
- Illinois Department of Public Health
- Cardiff University
- National Institutes of Health (4r01ca111882-10)
- University of Texas MD Anderson Cancer Center
- National Cancer Institute (R01-CA111882, CA111882)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Ovarian cancer diagnosis and treatment
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Keywords
- ovarian cancer, omental metastasis, mesothelial cells, extracellular matrix, tumor cell adhesion, tumor invasion, 3D culture models, type I collagen, type IV collagen, fibronectin, vitronectin, cell-cell contact
- MeSH terms
- Cell Adhesion, Cell Survival, Cells, Cultured, Epithelial Cells, Extracellular Matrix, Female, Fibroblasts, Humans, Immunohistochemistry, Keratins, Microscopy, Phase-Contrast, Models, Biological, Neoplasm Invasiveness, Omentum, Ovarian Neoplasms, Procollagen-Proline Dioxygenase, Tumor Cells, Cultured, Vimentin, CA-125 Antigen, Cell Culture Techniques, Cell Proliferation, beta Catenin
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