Transgenic mice with hematopoietic and lymphoid specific expression of Cre
Open accessAbstract
Bacteriophage P1 Cre/loxP based systems can be used to manipulate the genomes of mice in vivo and in vitro, allowing the generation of tissue‐specific conditional mutants. Wehave generated mouse lines expressing Cre recombinase in hematopoietic tissues using the vav regulatory elements, or in lymphoid cells using the hCD2 promoter and locus control region (LCR). The R26R‐EYFP Cre reporter mouse line was used to determine the pattern of Cre expression in each line and enabled the assessment of Cre activity at a single‐cell level. Analysis showed that the vav promoter elements were able to direct Cre‐mediated recombination in all cells of the hematopoietic system. The hCD2 promoter and LCR on the other hand were able to drive Cre‐mediated recombination only in T cells and B cells, but not in other hematopoietic cell types. Furthermore, in the appropriate tissues, deletion of the floxed target was complete in all cells, thereby excluding the possibility of variegated expression of the Cre transgene. Both of these Cre‐transgenic lines will be useful in generating tissue‐specific gene deletions within all the cells of hematopoietic or lymphoid tissues.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The Commd10Tg(Vav1-icre)A2Kio mouse line has been previously described.
“Trp53tm2Tyj (ref. 88), Commd10Tg(Vav1-icre)A2Kio (ref. 89; Jackson Laboratory, 008610) and Tg(Tal1-cre/ERT)42-056Jrg (ref. 90) have been previously described.”
From Single-cell multi-omics identifies chronic inflammation as a driver of TP53-mutant leukemic evolution (2023) · cites this paper as [89]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Gu, H., Zou, Y.-R., & Rajewsky, K. (1993) · Cell · doi:10.1016/0092-8674(93)90644-6 · Cited by 989 · see citing works
Hoxb8 Is Required for Normal Grooming Behavior in Mice
Greer, J. M., & Capecchi, M. R. (2002) · Neuron · doi:10.1016/s0896-6273(01)00564-5 · Cited by 401 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- Ogilvy, S., Metcalf, D., Print, C. G., Bath, M. L., Harris, A. W. and Adams, J. M., Constitutive Bcl-2 expression throughout the hematopoietic compartment affects multiple lineages and enhances progenitor cell survival. Proc. Natl. Acad. Sci. USA 1999. 96: 14943-14948.
- Schmidt, E. E., Taylor, D. S., Prigge, J. R., Barnett, S. and Capecchi, M. R., Illegitimate Cre-dependent chromosome rear- rangements in transgenic mouse spermatids. Proc. Natl. Acad. Sci. USA 2000. 97: 13702-13707.
Cited by
Sorted by citations
The autophagy protein Atg7 is essential for hematopoietic stem cell maintenance
Mortensen, M., Soilleux, E. J., Djordjevic, G., Tripp, R., Lutteropp, M., Sadighi-Akha, E., Stranks, A. J., Glanville, J., Knight, S., W. Jacobsen, S.-E., et al. (2011) · Journal of Experimental Medicine · doi:10.1084/jem.20101145 · Cited by 657 · see citing works
IDH1(R132H) mutation increases murine haematopoietic progenitors and alters epigenetics
Sasaki, M., Knobbe, C. B., Munger, J. C., Lind, E. F., Brenner, D., Brüstle, A., Harris, I. S., Holmes, R., Wakeham, A., Haight, J., et al. (2012) · Nature · doi:10.1038/nature11323 · Cited by 513 · see citing works
Epigenetic regulation of brain region-specific microglia clearance activity
Ayata, P., Badimon, A., Strasburger, H. J., Duff, M. K., Montgomery, S. E., Loh, Y.-H. E., Ebert, A., Pimenova, A. A., Ramirez, B. R., Chan, A. T., et al. (2018) · Nature Neuroscience · doi:10.1038/s41593-018-0192-3 · Cited by 460 · see citing works
Maternal retinoids control type 3 innate lymphoid cells and set the offspring immunity
van de Pavert, S. A., Ferreira, M., Domingues, R. G., Ribeiro, H., Molenaar, R., Moreira-Santos, L., Almeida, F. F., Ibiza, S., Barbosa, I., Goverse, G., et al. (2014) · Nature · doi:10.1038/nature13158 · Cited by 374 · see citing works
Minutti, C. M., Modak, R. V., Macdonald, F., Li, F., Smyth, D. J., Dorward, D. A., Blair, N., Husovsky, C., Muir, A., Giampazolias, E., et al. (2019) · Immunity · doi:10.1016/j.immuni.2019.01.008 · Cited by 228 · see citing works
Macrophages support pathological erythropoiesis in polycythemia vera and β-thalassemia
Ramos, P., Casu, C., Gardenghi, S., Breda, L., Crielaard, B. J., Guy, E., Marongiu, M. F., Gupta, R., Levine, R. L., Abdel-Wahab, O., et al. (2013) · Nature Medicine · doi:10.1038/nm.3126 · Cited by 227 · see citing works
Neuro-mesenchymal units control ILC2 and obesity via a brain–adipose circuit
Cardoso, F., Klein Wolterink, R. G. J., Godinho-Silva, C., Domingues, R. G., Ribeiro, H., da Silva, J. A., Mahú, I., Domingos, A. I., & Veiga-Fernandes, H. (2021) · Nature · doi:10.1038/s41586-021-03830-7 · Cited by 159 · see citing works
Rodriguez-Meira, A., Norfo, R., Wen, S., Chédeville, A. L., Rahman, H., O’Sullivan, J., Wang, G., Louka, E., Kretzschmar, W. W., Paterson, A., et al. (2023) · Nature Genetics · doi:10.1038/s41588-023-01480-1 · Cited by 158 · see citing works
Identification of subepithelial mesenchymal cells that induce IgA and diversify gut microbiota
Nagashima, K., Sawa, S., Nitta, T., Tsutsumi, M., Okamura, T., Penninger, J. M., Nakashima, T., & Takayanagi, H. (2017) · Nature Immunology · doi:10.1038/ni.3732 · Cited by 154 · see citing works
Savage, T. M., Fortson, K. T., de los Santos-Alexis, K., Oliveras-Alsina, A., Rouanne, M., Rae, S. S., Gamarra, J. R., Shayya, H., Kornberg, A., Cavero, R., et al. (2024) · Immunity · doi:10.1016/j.immuni.2024.01.009 · Cited by 115 · see citing works
Cell shape sensing licenses dendritic cells for homeostatic migration to lymph nodes
Alraies, Z., Rivera, C. A., Delgado, M.-G., Sanséau, D., Maurin, M., Amadio, R., Maria Piperno, G., Dunsmore, G., Yatim, A., Lacerda Mariano, L., et al. (2024) · Nature Immunology · doi:10.1038/s41590-024-01856-3 · Cited by 106 · see citing works
Fumarate hydratase is a critical metabolic regulator of hematopoietic stem cell functions
Guitart, A. V., Panagopoulou, T. I., Villacreces, A., Vukovic, M., Sepulveda, C., Allen, L., Carter, R. N., van de Lagemaat, L. N., Morgan, M., Giles, P., et al. (2017) · Journal of Experimental Medicine · doi:10.1084/jem.20161087 · Cited by 86 · see citing works
Riedelberger, M., Penninger, P., Tscherner, M., Seifert, M., Jenull, S., Brunnhofer, C., Scheidl, B., Tsymala, I., Bourgeois, C., Petryshyn, A., et al. (2020) · Cell Host & Microbe · doi:10.1016/j.chom.2020.01.023 · Cited by 64 · see citing works
Ferrer, I. R., West, H. C., Henderson, S., Ushakov, D. S., Santos e Sousa, P., Strid, J., Chakraverty, R., Yates, A. J., & Bennett, C. L. (2019) · Science Immunology · doi:10.1126/sciimmunol.aax8704 · Cited by 61 · see citing works
Nuclear envelope disruption triggers hallmarks of aging in lung alveolar macrophages
De Silva, N. S., Siewiera, J., Alkhoury, C., Nader, G. P. F., Nadalin, F., de Azevedo, K., Couty, M., Izquierdo, H. M., Bhargava, A., Conrad, C., et al. (2023) · Nature Aging · doi:10.1038/s43587-023-00488-w · Cited by 34 · see citing works
- Authors
- de Boer, J., Williams, A., Skavdis, G., Harker, N., Coles, M., Tolaini, M., Norton, T., Williams, K., Roderick, K., Potocnik, A. J., & Kioussis, D.
- Corresponding author
- Kioussis, Dimitris
- Venue
- European Journal of Immunology
- Publisher
- Wiley
- Published
- 2003-01-20
- Volume
- 33
- Issue
- 2
- Pages
- 314-325
- ISSN
- 0014-2980 (ISSN-L)
- 1521-4141
- OpenAlex
- W2002817805
- Cited by
- 739
- References
- 2
- Citations by year
- 2026: 23 · 2025: 44 · 2024: 41 · 2023: 32 · 2022: 55 · 2021: 56 · 2020: 35 · 2019: 44 · 2018: 45 · 2017: 38 · 2016: 35 · 2015: 42 · 2014: 51 · 2013: 39 · 2012: 36
- FWCI
- 3.68
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- European Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- Cre recombinase, Cre-loxP system, tissue-specific gene deletion, hematopoietic cells, lymphoid cells, locus control region, conditional mutants, T cells, B cells
- MeSH terms
- Animals, B-Lymphocytes, Bacterial Proteins, Hematopoietic Stem Cells, Humans, Luminescent Proteins, Lymphoid Tissue, Male, Mice, Transgenic, Microscopy, Fluorescence, Organ Specificity, Recombinant Fusion Proteins, T-Lymphocytes, Testis, Viral Proteins, Oncogene Proteins, Bacteriophage P1, Genes, Reporter, Gene Targeting, CD2 Antigens, Cell Lineage, Integrases, Locus Control Region, Mice, Proto-Oncogene Proteins c-vav
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
