Cited by 180interferon and immune responsesGenomics and Chromatin DynamicsDNA and Nucleic Acid Chemistry

Enhancer‐like interferon responsive sequences of the human and murine (2′‐5′) oligoadenylate synthetase gene promoters.

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Cohen, B., Peretz, D., Vaiman, D., Benech, P., Chebath, J.·1988·The EMBO Journal

Abstract

The human (2'-5') oligo(A) synthetase gene contains two independent cis-acting DNA elements, A and B, which act as transcriptional enhancers. Element A alone is not activated by IFN treatment. Element B alone confers IFN-inducibility to the herpes tk promoter. Two murine (2'-5') oligo(A) synthetase genes were isolated and their promoter sequences show high conservation of element A and B. A synthetic oligonucleotide, containing 16 bp of the human element B, or 14 bp of the homologue murine element B, was linked to a TK-CAT construct. These oligonucleotides were shown to be sufficient to activate the TK promoter in the presence of IFN. When multiple repeats of the interferon-responsive sequence (E-IRS) were cloned in 5' of the TK promoter, the activation ratio was increased. In vitro, specific binding of nuclear protein(s) is observed to the radiolabelled synthetic human E-IRS. This binding is competed by the addition of cold synthetic mouse E-IRS or fragments of genomic DNA containing the E-IRS.

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Crossref · 10.1002/j.1460-2075.1988.tb02958.x

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Authors
Cohen, B., Peretz, D., Vaiman, D., Benech, P., & Chebath, J.
Affiliations
Weizmann Institute of Science (IL)
Venue
The EMBO Journal
Published
1988-05-01
Volume
7
Issue
5
Pages
1411-1419
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
OpenAlex
W2344893292
Cited by
180
References
35
Citations by year
2024: 1 · 2020: 1 · 2018: 1 · 2015: 1 · 2014: 1 · 2013: 1
FWCI
11.64
Type
journal article
Language
en
Open access
Locations
  • The EMBO Journal (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords
2',5'-oligoadenylate synthetase, enhancers, cis-acting DNA elements, interferon induction, promoter elements, nuclear protein binding, sequence conservation
MeSH terms
Animals, Base Sequence, DNA, Enhancer Elements, Genetic, Multigene Family, Histocompatibility Antigens, Humans, Interferons, Molecular Sequence Data, Promoter Regions, Genetic, Protein Binding, Sequence Homology, Nucleic Acid, 2',5'-Oligoadenylate Synthetase, Mice

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