Enhancer‐like interferon responsive sequences of the human and murine (2′‐5′) oligoadenylate synthetase gene promoters.
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The human (2'-5') oligo(A) synthetase gene contains two independent cis-acting DNA elements, A and B, which act as transcriptional enhancers. Element A alone is not activated by IFN treatment. Element B alone confers IFN-inducibility to the herpes tk promoter. Two murine (2'-5') oligo(A) synthetase genes were isolated and their promoter sequences show high conservation of element A and B. A synthetic oligonucleotide, containing 16 bp of the human element B, or 14 bp of the homologue murine element B, was linked to a TK-CAT construct. These oligonucleotides were shown to be sufficient to activate the TK promoter in the presence of IFN. When multiple repeats of the interferon-responsive sequence (E-IRS) were cloned in 5' of the TK promoter, the activation ratio was increased. In vitro, specific binding of nuclear protein(s) is observed to the radiolabelled synthetic human E-IRS. This binding is competed by the addition of cold synthetic mouse E-IRS or fragments of genomic DNA containing the E-IRS.
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- Authors
- Cohen, B., Peretz, D., Vaiman, D., Benech, P., & Chebath, J.
- Affiliations
- Weizmann Institute of Science (IL)
- Venue
- The EMBO Journal
- Published
- 1988-05-01
- Volume
- 7
- Issue
- 5
- Pages
- 1411-1419
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC458391
- OpenAlex
- W2344893292
- Cited by
- 180
- References
- 35
- Citations by year
- 2024: 1 · 2020: 1 · 2018: 1 · 2015: 1 · 2014: 1 · 2013: 1
- FWCI
- 11.64
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- 2',5'-oligoadenylate synthetase, enhancers, cis-acting DNA elements, interferon induction, promoter elements, nuclear protein binding, sequence conservation
- MeSH terms
- Animals, Base Sequence, DNA, Enhancer Elements, Genetic, Multigene Family, Histocompatibility Antigens, Humans, Interferons, Molecular Sequence Data, Promoter Regions, Genetic, Protein Binding, Sequence Homology, Nucleic Acid, 2',5'-Oligoadenylate Synthetase, Mice
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