The DOI matches its Crossref record.
Quantitative keratinocyte assay detects two biological activities of human papillomavirus DNA and identifies viral types associated with cervical carcinoma
Abstract
Keratinocytes electroporated with human papillomavirus (HPV) DNA (HPV-6, 11, 16 and 18) exhibited an increased cellular proliferation which was quantitated as microcolony and macrocolony formation. However, only macrocolonies induced by HPV-16 or HPV-18 DNA (the two viral types most commonly found in human cervical carcinomas) gave rise to proliferating, poorly-stratified colonies when grown in the presence of serum and calcium. Hydrocortisone increased the frequency of these differentiation-resistant colonies, and studies showed that they were immortalized, contained one copy of viral DNA per cell, expressed three discrete species of viral RNA and synthesized the viral E7 protein. HPV-induced cellular proliferation and altered differentiation are therefore separable events and may represent the activity of different viral genes.
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“using primary human keratinocytes, it was shown that high-risk HPV genomes cause lifespan extension, inhibit keratinocyte differentiation and lead to cellular immortalization [85,86,87]”
From The Not-So-Good, the Bad and the Ugly: HPV E5, E6 and E7 Oncoproteins in the Orchestration of Carcinogenesis (2021) · cites this paper as [85]
Citations
Cited by
Association of Human Papillomavirus Types 16 and 18 E6 Proteins with p53
Werness, B. A., Levine, A. J., & Howley, P. M. (1990) · Science · doi:10.1126/science.2157286 · Cited by 2,562
Huibregtse, J. M., Scheffner, M., & Howley, P. M. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb04990.x · Cited by 879
Interaction of Papillomavirus E6 Oncoproteins with a Putative Calcium-Binding Protein
Chen, J. J., Reid, C. E., Band, V., & Androphy, E. J. (1995) · Science · doi:10.1126/science.7624774 · Cited by 275
Basukala, O., & Banks, L. (2021) · Viruses · doi:10.3390/v13101892 · Cited by 137
Metadata
- Authors
- Schlegel, R., Phelps, W. C., Zhang, Y. L., & Barbosa, M.
- Venue
- The EMBO Journal
- Published
- 1988-10-01
- Volume
- 7
- Issue
- 10
- Pages
- 3181-3187
- ISSN
- 0261-4189
- 1460-2075
- Cited by
- 189
- References
- 0
- Language
- en
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- CoChat. (2026). Citation check: Quantitative keratinocyte assay detects two biological activities of human papillomavirus DNA and identifies viral types associated with cervical carcinoma. https://app.cochat.ai/papers/10.1002/j.1460-2075.1988.tb03185.x
- The paper
- Schlegel, R., Phelps, W. C., Zhang, Y. L., & Barbosa, M. (1988). Quantitative keratinocyte assay detects two biological activities of human papillomavirus DNA and identifies viral types associated with cervical carcinoma. The EMBO Journal. https://doi.org/10.1002/j.1460-2075.1988.tb03185.x
