Cited by 879Cancer-related Molecular PathwaysCervical Cancer and HPV ResearchUbiquitin and proteasome pathways

A cellular protein mediates association of p53 with the E6 oncoprotein of human papillomavirus types 16 or 18.

Open access
Huibregtse, J.M., Scheffner, M., Howley, P.M.·1991·The EMBO Journal

Abstract

The E6 protein of human papillomavirus types 16 and 18 (HPV-16 and HPV-18) can stably associate with the p53 protein in vitro. In the presence of rabbit reticulocyte lysate, this association leads to the specific degradation of p53 through the ubiquitin-dependent proteolysis system. We have examined the E6-p53 complex in more detail and have found that association of E6 with p53 is mediated by an additional cellular factor. This factor is present in rabbit reticulocyte lysate, primary human keratinocytes and in each of five human cell lines examined. The factor is designated E6-AP, for E6-associated protein, based on the observation that the E6 proteins of HPV-16 and 18 can form a stable complex with the factor in the absence of p53, whereas p53 association with the factor can be detected only in the presence of E6. Gel filtration and coprecipitation experiments indicate that E6-AP is a monomeric protein of approximately 100 kDa.

Integrity signals

Registry

Crossref · 10.1002/j.1460-2075.1991.tb04990.x

Cites

49 of 49 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

The text we found for this paper looks like another document, so its claims and self-check weren’t run. The registry and citation checks still apply.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The text we found for this paper looks like another document, so the self-check wasn’t run.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Huibregtse, J. M., Scheffner, M., & Howley, P. M.
Affiliations
National Institutes of Health (US)
Venue
The EMBO Journal
Published
1991-12-01
Volume
10
Issue
13
Pages
4129-4135
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
OpenAlex
W140061535
Cited by
879
References
46
Citations by year
2026: 8 · 2025: 15 · 2024: 16 · 2023: 26 · 2022: 21 · 2021: 25 · 2020: 27 · 2019: 18 · 2018: 28 · 2017: 29 · 2016: 19 · 2015: 26 · 2014: 29 · 2013: 28 · 2012: 41
FWCI
5.96
Type
journal article
Language
en
Open access
Locations
  • The EMBO Journal (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Deutsche Forschungsgemeinschaft
  • National Cancer Institute
Topics
  • Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
  • Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
Keywords
p53, E6 oncoprotein, HPV16, HPV 18, UBE3A, ubiquitin-mediated proteolysis, p53 degradation, protein-protein interaction, keratinocytes, tumor suppressor
MeSH terms
Animals, Cell Line, Chromatography, Gel, DNA-Binding Proteins, Electrophoresis, Polyacrylamide Gel, Humans, Hydrolysis, Oncogene Proteins, Viral, Plasmids, Protein Binding, Proteins, Repressor Proteins, Reticulocytes, Tumor Suppressor Protein p53, Papillomaviridae

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review