The SIF binding element confers sis/PDGF inducibility onto the c‐fos promoter.
Open accessAbstract
The c-fos proto-oncogene is rapidly and transiently induced by a variety of extracellular stimuli. We have previously shown that conditioned media from v-sis transformed NRK cells rapidly induces a DNA binding protein which binds to a conserved sequence upstream of the human c-fos gene. We now show that purified recombinant c-sis/PDGF can induce this binding activity which we have termed SIF, for sis-inducible factor. Oligonucleotides which bind to the SIF protein will confer sis/PDGF inducibility onto a truncated, unresponsive c-fos promoter. However, sequences lying between -100 and -57 of the c-fos gene are required for this induction. The sis-responsive element functions independently of a region of dyad symmetry previously identified as the serum responsive element (SRE). The time course of c-fos expression driven by the sis-responsive element is similar to that mediated by the SRE. Unlike the SRE, which can respond to signals generated by sis/PDGF, serum or phorbol esters, the SIF binding element mediates c-fos induction only in response to sis/PDGF. The SRE and SIF elements function in an additive manner to stimulate the transcription of the c-fos gene in response to sis/PDGF.
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c-fos and junB expression is likely to be directly regulated by STAT3 because their full transactivation requires the STAT binding elements in their promoters.
“their expression is likely to be directly regulated by STAT3 because their full transactivation requires the STAT binding elements in their promoters (19”
From STAT3 Contributes to the Mitogenic Response of Hepatocytes during Liver Regeneration (2002) · cites this paper as [19]
The c-fos promoter experiments (section labeled Introduction; page unavailable) show that a SIF-binding element enhances c-sis/PDGF-induced transcription and that nonbinding mutations abolish induction in the tested constructs. The paper does not address junB or identify SIF as STAT3, and these experiments do not establish that STAT binding elements are required for full transactivation of both genes. (LLM, conf=0.87)
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- Hayes,T.E., Kitchen,A.M. and Cochran,B.H. (1987a) Proc. Natl. Acad. Sci. USA, 84, 1272-1276.
- Fink,J.S., Verhave,M., Kasper,S., Tsukada,T., Mandel,G. and Goodman,R.H. (1988) Proc. Natl. Acad. Sci. USA, 85, 6662-6666.
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- Authors
- Wagner, B. J., Hayes, T. E., Hoban, C. J., & Cochran, B. H.
- Affiliations
- Massachusetts Institute of Technology (US)
- ORCID
- Cochran, B. H.
- Venue
- The EMBO Journal
- Published
- 1990-12-01
- Volume
- 9
- Issue
- 13
- Pages
- 4477-4484
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC552240
- OpenAlex
- W127609430
- Cited by
- 592
- References
- 0
- Citations by year
- 2025: 2 · 2024: 1 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 4 · 2017: 1 · 2015: 2 · 2014: 4 · 2013: 5 · 2012: 4
- FWCI
- 3.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Rita Allen Foundation
- Pfizer
- Searle Scholars Program
- National Institutes of Health (CA42063)
- National Cancer Institute (P01CA042063, P01 CA 42063)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Keywords
- c-fos proto-oncogene, c-fos promoter, serum response element, transcriptional activation, serum response factor, ternary complex factor, DNA-binding proteins, transcriptional regulation, gene expression regulation, phorbol ester
- MeSH terms
- Proto-Oncogene Mas, Animals, Base Sequence, Cells, Cultured, DNA-Binding Proteins, Mice, Inbred BALB C, Molecular Sequence Data, Nuclear Proteins, Oncogenes, Promoter Regions, Genetic, Proto-Oncogene Proteins, Receptors, Cell Surface, Proto-Oncogene Proteins c-fos, Receptors, Platelet-Derived Growth Factor, Serum Response Factor, Mice
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