Oxidative damage induced in A549 cells by physically and chemically characterized air particulate matter (PM2.5) collected in Abidjan, Côte d'Ivoire
Abstract
Exposure to high levels of air pollution particulate matter (PM) is strongly associated with increased pulmonary morbidity and mortality. However, the underlying mechanisms of action whereby PM cause adverse health effects are still unclear. In developing countries, like in the sub‐Saharian region of Africa, people are often exposed to high PM levels. Hence, three PM 2.5 samples were collected in the District of Abidjan (Côte d'Ivoire), under rural, urban or industrial influences. Their most toxicologically relevant physical and chemical characteristics were determined – thereby showing that most of them were equal or smaller than 2.5 µm – and the influence of both natural (Ca, Na, Mg, Ti, etc.) and anthropic (Al, Fe, Mn, Cr, Pb, Zn, Cu, Ni, benzene and its derivatives, paraffins, etc.) emission sources. The toxicity induced by the three PM samples was studied through 5‐bromodeoxyuridine incorporation to DNA, mitochondrial dehydrogenase activity and extracellular lactate dehydrogenase activity. Hence, effect concentrations at 10 and 50% (EC 10 and EC 50, respectively) were as follows: (i) rural PM – EC 10 = 5.91 µg cm−2 and EC 50 = 29.55 µg cm−2; (ii) urban PM – EC 10 = 5.45 µg cm−2 and EC 50 = 27.23 µg cm−2; and (iii) industrial PM – EC 10 = 6.86 µg cm−2 and EC 50 = 34.29 µg cm−2. Moreover, PM‐induced oxidative damage in A549 cells was observed through the induction of lipid peroxidation, the alteration of superoxide dismutase activity, and the disruption of glutathione status. Both the transition metals and the organic chemicals within the three collected PM samples under study might be involved in the oxidative damage and, therefore, the toxicity they induced in A549 cells. Copyright © 2009 John Wiley & Sons, Ltd.
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Oxidative stress is a common mechanism of PM2.5-induced damage.
“Accumulated evidence indicates that oxidative stress is a common mechanism of PM2.5-induced damage (Gualtieri et al. 2012; Kouassi et al. 2010).”
From Particulate matter 2.5 damages skin cells by inducing oxidative stress, subcellular organelle dysfunction, and apoptosis (2018) · cites this paper as Kouassi et al. 2010
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- Authors
- Kouassi, K. S., Billet, S., Garçon, G., Verdin, A., Diouf, A., Cazier, F., Djaman, J., Courcot, D., & Shirali, P.
- Affiliations
- Université du littoral côte d'opale (FR)
- Cheikh Anta Diop University (SN)
- Institut Pasteur de Côte d'Ivoire (CI)
- Corresponding author
- Shirali, Pirouz
- Venue
- Journal of Applied Toxicology
- Publisher
- Wiley
- Published
- 2009-11-27
- Volume
- 30
- Issue
- 4
- Pages
- 310-320
- ISSN
- 0260-437X (ISSN-L)
- 1099-1263
- OpenAlex
- W2017673350
- Cited by
- 89
- References
- 50
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 6 · 2023: 8 · 2022: 10 · 2021: 7 · 2020: 6 · 2019: 9 · 2018: 11 · 2017: 4 · 2016: 4 · 2015: 3 · 2014: 3 · 2013: 6 · 2012: 2
- FWCI
- 1.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministère de l'Enseignement Supérieur et de la Recherche
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
- Physical Sciences › Earth and Planetary Sciences › Atmospheric Science › Atmospheric chemistry and aerosols
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Indoor Air Quality and Microbial Exposure
- Keywords
- PM2.5, particulate matter, oxidative stress, A549 cells, lipid peroxidation, superoxide dismutase, glutathione, transition metals, organic pollutants, benzene, Abidjan, Côte d'Ivoire, sub-Saharan Africa, DNA damage, lactate dehydrogenase, mitochondrial dehydrogenases
- MeSH terms
- Air Pollutants, Antioxidants, Cell Survival, Environmental Monitoring, Humans, Cote d'Ivoire, Lipid Peroxides, Microscopy, Electron, Scanning, Particle Size, Surface Properties, Urbanization, Lipid Peroxidation, Oxidative Stress, Cell Culture Techniques, Cell Line, Tumor, Particulate Matter
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