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Cited by 81Collagen: Extraction and CharacterizationTissue Engineering and Regenerative MedicineBone Tissue Engineering Materials

Assessment of the biocompatibility and stability of a gold nanoparticle collagen bioscaffold

Grant, Sheila A., Spradling, Claire S., Grant, Daniel N., Fox, Derek B., Jimenez, Luis, Grant, David A., Rone, Rebecca J.·2014·Journal of Biomedical Materials Research Part A
DOI 10.1002/jbm.a.34698
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Abstract

Collagen has been utilized as a scaffold for tissue engineering applications due to its many advantageous properties. However, collagen in its purified state is mechanically weak and prone to rapid degradation. To mitigate these effects, collagen can be crosslinked. Although enhanced mechanical properties and stability can be achieved by crosslinking, collagen can be rendered less biocompatible either due to changes in the overall microstructure or due to the cytotoxicity of the crosslinkers. We have investigated crosslinking collagen using gold nanoparticles (AuNPs) to enhance mechanical properties and resistance to degradation while also maintaining its natural microstructure and biocompatibility. Rat tail type I collagen was crosslinked with AuNPs using a zero-length crosslinker, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). Several characterization studies were performed including electron microscopy, collagenase assays, ROS assays, and biocompatibility assays. The results demonstrated that AuNP-collagen scaffolds had increased resistance to degradation as compared to non-AuNP-collagen while still maintaining an open microstructure. Although the biocompatibility assays showed that the collagen and AuNP-collagen scaffolds are biocompatible, the AuNP-collagen demonstrated enhanced cellularity and glycoaminoglycans (GAG) production over the collagen scaffolds. Additionally, the Reactive Oxygen Species (ROS) assays indicated the ability of the AuNP-collagen to reduce oxidation. Overall, the AuNP-collagen scaffolds demonstrated enhanced biocompatibility and stability over non-AuNP scaffolds.

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  • When scaffolds are seeded with cells, the rate of bone formation drastically increases.

    “However, when the scaffolds were seeded with the cells, the rate of bone formation was found to drastically increase [60].”

    From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [60]

  • Scaffolds placed inside a biological system without seeding any cells induce cell differentiation and bone formation.

    “Even though when scaffolds were placed inside the biological system without seeding any cells, they were found to induce cell differentiation and bone formation.”

    From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [60]

Cites

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Research works printed but not linked: 5

  1. Chou CW, 2008, Biostability and biocompatibility of poly(ether)urethane containing gold or silver nanoparticles in a porcine model, J Biomed Mater Res Part A, 84, 85
  2. 10.2217/17435889.1.2.169 · DOI
  3. 10.1023/B:JMSM.0000036281.47596.cc · DOI
  4. 10.1089/10763270260424114 · DOI
  5. 10.1006/excr.2002.5498 · DOI

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Related

Authors
Grant, S. A., Spradling, C. S., Grant, D. N., Fox, D. B., Jimenez, L., Grant, D. A., & Rone, R. J.
Affiliations
University of Missouri (US)
ORCID
Grant, Sheila A.
Venue
Journal of Biomedical Materials Research Part A
Publisher
Wiley
Published
2013-05-14
Volume
102
Issue
2
Pages
332-339
ISSN
  • 1549-3296 (ISSN-L)
  • 1552-4965
DOI
10.1002/jbm.a.34698
OpenAlex
W1851802643
Cited by
81
References
32
Citations by year
2026: 2 · 2025: 6 · 2024: 3 · 2023: 6 · 2022: 3 · 2021: 7 · 2020: 4 · 2019: 8 · 2018: 9 · 2017: 13 · 2016: 8 · 2015: 7 · 2014: 4 · 2013: 1
FWCI
2.55
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Journal of Biomedical Materials Research Part A (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
Keywords
gold nanoparticles, collagen, collagen cross-linking, scaffold stability, biocompatibility, type I collagen, rat tail collagen, reactive oxygen species, antioxidant activity, tissue engineering scaffolds
MeSH terms
Animals, Cell Line, Collagen, Gold, Materials Testing, Reactive Oxygen Species, Rats, Metal Nanoparticles, Tissue Scaffolds

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