RETRACTED: Downregulation of miR‐122 in the rodent and human hepatocellular carcinomas
Open accessAbstract
MicroRNAs (miRs) are conserved small non‐coding RNAs that negatively regulate gene expression. The miR profiles are markedly altered in cancers and some of them have a causal role in tumorigenesis. Here, we report changes in miR expression profile in hepatocellular carcinomas (HCCs) developed in male Fisher rats‐fed folic acid, methionine, and choline‐deficient (FMD) diet. Comparison of the miR profile by microarray analysis showed altered expression of some miRs in hepatomas compared to the livers from age‐matched rats on the normal diet. While let‐7a, miR‐21, miR‐23, miR‐130, miR‐190, and miR‐17‐92 family of genes was upregulated, miR‐122, an abundant liver‐specific miR, was downregulated in the tumors. The decrease in hepatic miR‐122 was a tumor‐specific event because it did not occur in the rats switched to the folate and methyl‐adequate diet after 36 weeks on deficient diet, which did not lead to hepatocarcinogenesis. miR‐122 was also silent in a transplanted rat hepatoma. Extrapolation of this study to human primary HCCs revealed that miR‐122 expression was significantly (P = 0.013) reduced in 10 out of 20 tumors compared to the pair‐matched control tissues. These findings suggest that the downregulation of miR‐122 is associated with hepatocarcinogenesis and could be a potential biomarker for liver cancers. J. Cell. Biochem. 99: 671–678, 2006. © 2006 Wiley‐Liss, Inc.
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In hepatocellular carcinoma, miR-23a/b are most probably derived from adipocytes and are upregulated.
“miR-23a/b are most probably derived from adipocytes and are upregulated.”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [71]
The Results (Table I; page unavailable) report that miR-23a and miR-23b were upregulated in three rat hepatocellular carcinomas. The paper does not support the claim that they are probably derived from adipocytes, and its human-tumor analysis addresses miR-122 rather than miR-23a/b. (LLM, conf=0.90)
Experimental — accuracy not yet measuredmiR-23a/b are transferred to hepatocellular carcinoma cancer cells via exosomes, promoting the growth and migration of HCC cells.
“These miRNAs are then transferred to cancer cells via exosomes, thus promoting the growth and migration of HCC cells.”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [71]
The Results (page unavailable) report that miR-23a and miR-23b were elevated in rat HCC tumors and suggest a potential role for miR-23 in tumorigenesis. They do not report exosomal transfer to cancer cells or show that these miRNAs promote HCC cell growth or migration. (LLM, conf=0.87)
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- Authors
- Kutay, H., Bai, S., Datta, J., Motiwala, T., Pogribny, I., Frankel, W., Jacob, S. T., & Ghoshal, K.
- Affiliations
- The Ohio State University (US)
- National Center for Toxicological Research (US)
- Corresponding author
- Ghoshal, Kalpana
- Venue
- Journal of Cellular Biochemistry
- Publisher
- Wiley
- Published
- 2006-06-30
- Volume
- 99
- Issue
- 3
- Pages
- 671-678
- ISSN
- 0730-2312 (ISSN-L)
- 1097-4644
- PMC
- PMC3033198
- OpenAlex
- W1979912768
- Cited by
- 608
- References
- 1
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 9 · 2023: 19 · 2022: 18 · 2021: 21 · 2020: 16 · 2019: 27 · 2018: 27 · 2017: 23 · 2016: 42 · 2015: 49 · 2014: 48 · 2013: 45 · 2012: 51
- FWCI
- 13.71
- Type
- journal article
- Language
- en
- Status
- Retracted
- Update notices
- retraction (2025-04-28)
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Cellular Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Cancer Institute (R01CA086978, CA 86978)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Health Sciences › Medicine › Hepatology › Hepatocellular Carcinoma Treatment and Prognosis
- Keywords
- miR-122, hepatocellular carcinoma, miRNA expression profiling, hepatocarcinogenesis, folate deficiency, let-7a, miR-21, tumor markers, liver cancer
- MeSH terms
- Animals, Choline Deficiency, Diet, Folic Acid Deficiency, Carcinoma, Hepatocellular, Humans, Liver Neoplasms, Male, Methionine, Rats, Inbred F344, Gene Expression Regulation, Neoplastic, Gene Expression Profiling, MicroRNAs, Microarray Analysis, Rats
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