Three-dimensional Growth and Differentiation of Ovarian Tumor Cell Line in High Aspect Rotating-wall Vessel: Morphologic and Embryologic Considerations
Abstract
Cancer of the ovary is the leading cause of death from gynecologic malignancy. To understand better these aggressive tumors, the development of in vitro models to study human ovarian cancer is critical. However, the establishment of long-term cell lines has been difficult, due to the generalized poor survival of patient tumor cells grown in primary culture. Satisfactory culture systems for ovarian tumor cells have therefore been limited. To study cellular interactions involved in the growth and differentiation of these tumors, a cell line was established from a mixed müllerian tumor of the ovary. This cell line, designated LN1, was cultured on microcarrier beads in the high aspect rotating-wall vessel. The tumor cells grown in this vessel readily proliferated without a requirement for cocultivation with a supportive cell layer. Evaluation of cellular morphology by phase contrast light microscopy and scanning electron microscopy revealed the presence of three-dimensional multicellular aggregates consisting of multiple cell-coated beads bridged together, as well as scattered aggregates of LN1 cells proliferating as spheroids free in suspension. In contrast to conventional culture systems, culture in the high aspect rotating-wall vessel facilitated the generation of multiple cell types that could be recovered. These results illustrate the ability of this culture system to provide the biological conditions necessary for pluripotent cell growth.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
LN1 cells grown in a horizontally-oriented high aspect rotating vessel (HARV) with microcarrier beads as a scaffold showed growth selection for certain cell types from a mixed population in 3D compared to 2D.
“LN1 cells, derived from a mixed Mullerian tumor, were grown in a horizontally-oriented high aspect rotating vessel (HARV) with microcarrier beads as a scaffold to investigate the potential for metastatic growth as spheroids or aggregates (Becker et al., 1993). This study also showed that there is growth selection for certain cell types from a mixed population in 3D compared to 2D.”
From Three-dimensional modelling of ovarian cancer: from cell lines to organoids for discovery and personalized medicine (2022) · cites this paper as Becker et al., 1993
Cites
Analysis of a human tumor-associated glycoprotein (TAG-72) identified by monoclonal antibody B72.3.
Johnson, V. G., Schlom, J., Paterson, A. J., Bennett, J., Magnani, J. L., & Colcher, D. M. (1986) · PubMed · Cited by 406 · see citing works
Schwarz, R. P., Goodwin, T. J., & Wolf, D. A. (1992) · Journal of Tissue Culture Methods · doi:10.1007/bf01404744 · Cited by 399 · see citing works
The secondary Müllerian system.
Lauchlan, S. (1972) · Obstetrical and Gynecological Survey · doi:10.1097/00006254-197203000-00001 · Cited by 371 · see citing works
Carcinosarcomas and mixed mesodermal tumors of the ovary
Dehner, L. P., Norris, H. J., & Taylor, H. B. (1971) · Cancer · doi:10.1002/1097-0142(197101)27:1 · Cited by 125 · see citing works
Assessment of tumor cell kinetics by immunohistochemistry in carcinoma of breast
McGurrin, J. F., Doria, M. I., Dawson, P. J., Karrison, T., Stein, H. O., & Franklin, W. A. (1987) · Cancer · doi:10.1002/1097-0142(19870515)59:10 · Cited by 121 · see citing works
Morphologic differentiation of colon carcinoma cell lines HT-29 and HT-29KM in rotating-wall vessels
Goodwin, T. J., Jessup, J. M., & Wolf, D. A. (1992) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/bf02631079 · Cited by 114 · see citing works
UTERINE SARCOMAS: HISTOGENESIS AND TAXONOMY
Ober, W. B. (1959) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1959.tb44576.x · Cited by 106 · see citing works
Mesenchymal sarcomas of the uterus
Ober, W. B., & Tovell, H. M. M. (1959) · American Journal of Obstetrics and Gynecology · doi:10.1016/0002-9378(59)90225-x · Cited by 64 · see citing works
Das traubige Sarcom der Cervix uteri
Pfannenstiel, J. (1892) · Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin · doi:10.1007/bf01941375 · Cited by 60 · see citing works
Siegel, K., Mesagno, F. P., Karus, D. G., & Christ, G. H. (1992) · Cancer · doi:10.1002/1097-0142(19920401)69:7 · Cited by 58 · see citing works
Cystic pulmonary metastatic sarcoma
Traweek, S. T., Rotter, A. J., Swartz, W., & Azumi, N. (1990) · Cancer · doi:10.1002/1097-0142(19900415)65:8 · Cited by 58 · see citing works
Histogenesis and culture of human uterine carcinosarcoma.
Ishiwata, I., Ishiwata, C., Nagayama, T., & Ishikawa, H. (1981) · PubMed · Cited by 37 · see citing works
Surface (germinal) epithelium and related ovarian neoplasms.
Blaustein, A. (1981) · PubMed · Cited by 33 · see citing works
Puts, J. J. G., Moesker, O., Aldeweireldt, J., Vooijs, G. P., & Ramaekers, F. C. S. (1987) · International Journal of Gynecological Pathology · doi:10.1097/00004347-198709000-00007 · Cited by 28 · see citing works
State of the art in comparative cancer clinical trials
Begg, C. B., Pocock, S., Freedman, L. S., & Zelen, M. (1987) · Cancer · doi:10.1002/1097-0142(19871201)60:11 · Cited by 20 · see citing works
van Haaften‐Day, C., Russell, P., Carr, S., & Wright, L. C. (1988) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/bf02620867 · Cited by 10 · see citing works
Boven (1984) · doi:10.1002/1097-0142(19840101)53:1
Flagged first, then by citations
Cited by
Sorted by citations
Three-dimensional tissue culture models in cancer biology
Kim, J. B. (2005) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2005.05.002 · Cited by 613 · see citing works
Growing tissues in microgravity
Unsworth, B. R., & Lelkes, P. I. (1998) · Nature Medicine · doi:10.1038/nm0898-901 · Cited by 393 · see citing works
Hickman, J. A., Graeser, R., Hoogt, R. D., Vidic, S., Brito, C., Gutekunst, M., & van der Kuip, H. (2014) · Biotechnology Journal · doi:10.1002/biot.201300492 · Cited by 379 · see citing works
Yu, X., Botchwey, E. A., Levine, E. M., Pollack, S. R., & Laurencin, C. T. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402532101 · Cited by 296 · see citing works
Burleson, K. M., Casey, R. C., Skubitz, K. M., Pambuccian, S. E., Oegema, T. R., & Skubitz, A. P. N. (2004) · Gynecologic Oncology · doi:10.1016/j.ygyno.2003.12.034 · Cited by 269 · see citing works
Hutmacher, D. W., Schantz, T., Zein, I., Ng, K. W., Teoh, S. H., & Tan, K. C. (2001) · Journal of Biomedical Materials Research · doi:10.1002/1097-4636(200105)55:2 · Cited by 1,438 · see citing works
Ingram, M., Techy, G. B., Saroufeem, R. M. G., Yazan, O., Narayan, K. S., Goodwin, T. J., & Spaulding, G. F. (1997) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-997-0064-8 · Cited by 225 · see citing works
β1-Integrins Regulate the Formation and Adhesion of Ovarian Carcinoma Multicellular Spheroids
Casey, R. C., Burleson, K. M., Skubitz, K. M., Pambuccian, S. E., Oegema, T. R., Ruff, L. E., & Skubitz, A. P. N. (2001) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)63058-1 · Cited by 225 · see citing works
Microgravity tissue engineering
Freed, L. E., & Vunjak‐Novakovic, G. (1997) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-997-0009-2 · Cited by 202 · see citing works
Akins, R. E., Boyce, R., Madonna, M. L., Schroedl, N. A., Gonda, S. R., McLaughlin, T., & Hartzell, C. R. (1999) · Tissue Engineering · doi:10.1089/ten.1999.5.103 · Cited by 171 · see citing works
Cultivation of cell–polymer tissue constructs in simulated microgravity
Freed, L. E., & Vunjak‐Novakovic, G. (1995) · Biotechnology and Bioengineering · doi:10.1002/bit.260460403 · Cited by 171 · see citing works
Disaggregation and invasion of ovarian carcinoma ascites spheroids
Burleson, K. M., Boente, M. P., Pambuccian, S. E., & Skubitz, A. P. N. (2006) · Journal of Translational Medicine · doi:10.1186/1479-5876-4-6 · Cited by 146 · see citing works
Burleson, K. M., Hansen, L. K., & Skubitz, A. P. N. (2005) · Clinical & Experimental Metastasis · doi:10.1007/s10585-004-5768-5 · Cited by 144 · see citing works
The Effects of Weightlessness on the Human Organism and Mammalian Cells
Pietsch, J., Bauer, J. W., Egli, M., Infanger, M., Wise, P. M., Ulbrich, C., & Grimm, D. G. (2011) · Current Molecular Medicine · doi:10.2174/156652411795976600 · Cited by 141 · see citing works
Using space-based investigations to inform cancer research on Earth
Becker, J. L., & Souza, G. R. (2013) · Nature reviews. Cancer · doi:10.1038/nrc3507 · Cited by 141 · see citing works
Storrie, H., & Stupp, S. I. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.01.043 · Cited by 139 · see citing works
Induction of three-dimensional assembly of human liver cells by simulated microgravity
Khaoustov, V. I., Darlington, G., Soriano, H. E., Krishnan, B., Risin, D., Pellis, N. R., & Yoffe, B. (1999) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-999-0060-2 · Cited by 114 · see citing works
Granet, C., Laroche, N., Vico, L., Alexandre, C., & Lafage‐Proust, M. (1998) · Medical & Biological Engineering & Computing · doi:10.1007/bf02523224 · Cited by 108 · see citing works
Zhau, H. E., Goodwin, T. J., Chang, S.-M., Baker, T. L., & Chung, L. W. K. (1997) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-997-0008-3 · Cited by 86 · see citing works
Changes in gravity inhibit lymphocyte locomotion through type I collagen
Pellis, N. R., Goodwin, T. J., Risin, D., McIntyre, B. W., Pizzini, R. P., Cooper, D. A., Baker, T. L., & Spaulding, G. F. (1997) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-997-0012-7 · Cited by 82 · see citing works
Lelkes, P. I., Galvan, D. L., Hayman, G. T., Goodwin, T. J., Chatman, D. Y., Cherian, S., Garcia, R. M. G., & Unsworth, B. R. (1998) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-998-0008-y · Cited by 81 · see citing works
LONG TERM ORGAN CULTURE OF HUMAN PROSTATE TISSUE IN A NASA-DESIGNED ROTATING WALL BIOREACTOR
Margolis, L. B., Hatfill, S. J., Chuaqui, R. F., Vocke, C. D., Emmert‐Buck, M. R., Linehan, W. M., & Duray, P. H. (1999) · The Journal of Urology · doi:10.1016/s0022-5347(01)62134-7 · Cited by 74 · see citing works
John, G. T., & Heinzle, E. (2001) · Biotechnology and Bioengineering · doi:10.1002/1097-0290(20010320)72:6 · Cited by 39 · see citing works
Yee, C., Dickson, K.-A., Muntasir, M. N., Ma, Y., & Marsh, D. J. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.836984 · Cited by 66 · see citing works
Corydon, T. J., Mann, V., Slumstrup, L., Kopp, S., Sahana, J., Askou, A. L., Magnusson, N. E., Echegoyen, D., Bek, T., Sundaresan, A., et al. (2016) · Cellular Physiology and Biochemistry · doi:10.1159/000452520 · Cited by 66 · see citing works
Rotary Cell Culture System (RCCS): A new Method for Cultivating Hepatocytes on Microcarriers
Mitteregger, R., Vogt, G., Roßmanith, E., & Falkenhagen, D. (1999) · The International Journal of Artificial Organs · doi:10.1177/039139889902201207 · Cited by 64 · see citing works
Qiu, Q., Ducheyne, P., Gao, H., & AYYASWAMY, P. S. (1998) · Tissue Engineering · doi:10.1089/ten.1998.4.19 · Cited by 64 · see citing works
Three-dimensional culture of bovine chondrocytes in rotating-wall vessels
Baker, T. L., & Goodwin, T. J. (1997) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-997-0006-5 · Cited by 61 · see citing works
Three-dimensional endothelial-tumor epithelial cell interactions in human cervical cancers
Chopra, V., Dinh, T., & Hannigan, E. V. (1997) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-997-0061-y · Cited by 52 · see citing works
Goodwin, T. J., Prewett, T. L., Spaulding, G. F., & Becker, J. L. (1997) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-997-0007-4 · Cited by 50 · see citing works
Marlovits, S., Tichy, B., Truppe, M. J., Gruber, D., & Schlegel, W. (2003) · The International Journal of Artificial Organs · doi:10.1177/039139880302600407 · Cited by 47 · see citing works
Characterization of Primary Breast Carcinomas Grown in Three-Dimensional Cultures
Becker, J. L., & Blanchard, D. K. (2007) · Journal of Surgical Research · doi:10.1016/j.jss.2007.03.016 · Cited by 43 · see citing works
Polymeric Hydrogels for In Vitro 3D Ovarian Cancer Modeling
Braccini, S., Tacchini, C., Chiellini, E., & Puppi, D. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23063265 · Cited by 42 · see citing works
Cultures of human liver cells in simulated microgravity environment
Yoffe, B., Darlington, G., Soriano, H. E., Krishnan, B., Risin, D., Pellis, N. R., & Khaoustov, V. I. (1999) · Advances in Space Research · doi:10.1016/s0273-1177(99)00079-4 · Cited by 38 · see citing works
Tissue engineering lamb heart valve leaflets
Breuer, C. K., Shinoka, T., Tanel, R. E., Zünd, G., Mooney, D. J., Ma, P. X., Miura, T., Colan, S. D., Langer, R., Mayer, J. E., et al. (1996) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(19960605)50:5 · Cited by 88 · see citing works
Nelson, L. J., Walker, S. W., Hayes, P. C., & Plevris, J. N. (2010) · Cells Tissues Organs · doi:10.1159/000308893 · Cited by 37 · see citing works
Generation of 3D Retina-Like Structures from a Human Retinal Cell Line in a NASA Bioreactor
Dutt, K., Harris‐Hooker, S., Ellerson, D., Layne, D., Kumar, R., & Hunt, R. C. (2003) · Cell Transplantation · doi:10.3727/000000003108747334 · Cited by 36 · see citing works
Cultivation of lacrimal gland acinar cells in a microgravity environment
Schrader, S., Kremling, C., Klinger, M. H. F., Laqua, H., & Geerling, G. (2009) · British Journal of Ophthalmology · doi:10.1136/bjo.2008.137927 · Cited by 35 · see citing works
Invasion of Human Tissue Ex Vivo byBorrelia burgdorferi
Duray, P. H., Yin, S., Ito, Y., Bezrukov, L., Cox, C., Cho, M., Fitzgerald, W., Dorward, D. W., Zimmerberg, J. J., & Margolis, L. B. (2005) · The Journal of Infectious Diseases · doi:10.1086/429632 · Cited by 34 · see citing works
Effect of glutamine limitation on the death of attached Chinese hamster ovary cells
Sanfeliu, A., & Stephanopoulos, G. N. (1999) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(19990705)64:1 · Cited by 53 · see citing works
Wang, S. S. ‐., & Good, T. A. (2001) · Journal of Cellular Biochemistry · doi:10.1002/jcb.1252 · Cited by 32 · see citing works
Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations
Wang, Y., Kim, M. H., Tabaei, S. R., Park, J. H., Na, K., Chung, S. H., Zhdanov, V. P., & Cho, N. (2016) · PLoS ONE · doi:10.1371/journal.pone.0161915 · Cited by 31 · see citing works
Multicellular ovarian cancer spheroids: novel 3D model to mimic tumour complexity
Flörkemeier, I., Antons, L. K., Weimer, J. P., Hedemann, N., Rogmans, C., Krüger, S., Scherließ, R., Dempfle, A., Arnold, N., Maass, N., et al. (2024) · Scientific Reports · doi:10.1038/s41598-024-73680-6 · Cited by 30 · see citing works
Schönthal, A. H., Swenson, S. D., Chen, T. C., & Markland, F. S. (2020) · Biochemical Pharmacology · doi:10.1016/j.bcp.2020.114149 · Cited by 30 · see citing works
Drago‐Ferrante, R., Fiore, R. D., Karouia, F., Subbannayya, Y., Das, S., Mathyk, B. A., Arif, S., Guevara-Cerdán, A. P., Seylani, A., Galsinh, A. S., et al. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23137465 · Cited by 30 · see citing works
Consolo, F., Fiore, G. B., Truscello, S., Caronna, M., Morbiducci, U., Montevecchi, F. M., & Redaelli, A. C. L. (2009) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2008.0213 · Cited by 29 · see citing works
Dutt, K., Sanford, G. L., Harris‐Hooker, S., Brako, L. A., Kumar, R., Sroufe, A. E., & Melhado, C. (2003) · Tissue Engineering · doi:10.1089/107632703322495547 · Cited by 28 · see citing works
Yamamoto, S., Hotta, M. M., Okochi, M., & Honda, H. (2014) · PLoS ONE · doi:10.1371/journal.pone.0103502 · Cited by 26 · see citing works
Becker, J. L., Papenhausen, P. R., & Widen, R. H. (1997) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-997-0001-x · Cited by 24 · see citing works
Long, J. P., Pierson, S., & Hughes, J. H. (1999) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-999-0043-3 · Cited by 19 · see citing works
Leung, S., Kim, J., Musson, D. S., McGlashan, S. R., Cornish, J., Anderson, I. A., & Shim, V. (2021) · Journal of Biomechanical Engineering · doi:10.1115/1.4051116 · Cited by 17 · see citing works
O’Connor, K. C., Enmon, R. M., Dotson, R. S., Primavera, A. C., & Clejan, S. (1997) · Tissue Engineering · doi:10.1089/ten.1997.3.161 · Cited by 17 · see citing works
Cultivation of fall armyworm ovary cells in simulated microgravity
Francis, K. M., O’Connor, K. C., & Spaulding, G. F. (1997) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-997-0002-9 · Cited by 16 · see citing works
Simulated Microgravity and Hypergravity Attenuate Heart Tissue Development in Explant Culture
Lwigale, P. Y., Thurmond, J. E., Norton, W. N., Spooner, B. S., & Wiens, D. J. (2000) · Cells Tissues Organs · doi:10.1159/000016780 · Cited by 15 · see citing works
Three-dimensional transgenic cell model to quantify genotoxic effects of space environment
Gonda, S. R., Wu, H., Pingerelli, P. L., & Glickman, B. W. (2001) · Advances in Space Research · doi:10.1016/s0273-1177(01)00010-2 · Cited by 15 · see citing works
Saporta, S., Willing, A. E., Shamekh, R., Bickford, P. C., Paredes, D., & Cameron, D. F. (2004) · Brain Research Bulletin · doi:10.1016/j.brainresbull.2004.09.003 · Cited by 15 · see citing works
Whole organ culture of the postnatal sensory inner ear in simulated microgravity
Hahn, H., Müller, M. C., & Löwenheim, H. (2008) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2008.02.004 · Cited by 14 · see citing works
Current approaches for the regeneration and reconstruction of ocular surface in dry eye
Singh, V. K., Sharma, P., Vaksh, U. K. S., & Chandra, R. (2022) · Frontiers in Medicine · doi:10.3389/fmed.2022.885780 · Cited by 14 · see citing works
Proliferation behavior of E. coli in a three-dimensional in vitro tumor model
Elliott, N. T., Lee, T., You, L., & Yuan, F. (2011) · Integrative Biology · doi:10.1039/c0ib00137f · Cited by 14 · see citing works
Influence of simulated microgravity on the longevity of insect-cell culture
Cowger, N. L., O’Connor, K. C., & Bivins, J. E. (1997) · Enzyme and Microbial Technology · doi:10.1016/s0141-0229(96)00153-6 · Cited by 14 · see citing works
Simulated microgravity impairs respiratory burst activity in human promyelocytic cells
Hughes, J. H., & Long, J. P. (2001) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/bf02577531 · Cited by 13 · see citing works
Hormonal Modulation of Ishikawa Cells During Three-Dimensional Growth In Vitro
PINELLI, D., DRAKE, J., WILLIAMS, M., Cavanagh, D., & Becker, J. L. (1998) · Journal of the Society for Gynecologic Investigation · doi:10.1016/s1071-5576(98)00015-x · Cited by 10 · see citing works
Generation of Two- and Three-Dimensional Lacrimal Gland Constructs
Schrader, S., Liu, L., Kasper, K., & Geerling, G. (2010) · Developments in ophthalmology · doi:10.1159/000315019 · Cited by 7 · see citing works
Three-dimensional in vitro models in head and neck cancer: current trends and applications
Amiri, M., Tabatabai, T. S., Seifi, Z., Rostaminasab, G., Mikaeili, A., Hosseini, F., & Rezakhani, L. (2025) · Medical Oncology · doi:10.1007/s12032-025-02737-x · Cited by 4 · see citing works
Fetal thymic organ culture in rotating bioreactors
Tabourn, B., & Spain, L. M. (1999) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-999-0034-4 · Cited by 4 · see citing works
Study of Rotary Cell Culture System-Induced Microgravity Effects on Cancer Biomarkers
Singh, R., & Singh, R. P. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-1896-7_10 · Cited by 2 · see citing works
Saleh, R. I., & Cha, C. (2025) · Biofabrication · doi:10.1088/1758-5090/ade7b1 · Cited by 2 · see citing works
Chakrabarti, A., Stoecker, A., & Schatten, H. (1995) · doi:10.2514/6.1995-1059 · Cited by 2 · see citing works
Rotating-Wall Vessels for Cell Culture
Qiu, Q., AYYASWAMY, P. S., & Ducheyne, P. (2011) · Comprehensive Biomaterials · doi:10.1016/b978-0-08-055294-1.00165-3 · Cited by 2 · see citing works
Krüger, M., Bauer, J. W., & Grimm, D. G. (2017) · SpringerBriefs in space life sciences · doi:10.1007/978-3-319-64054-9_7 · Cited by 2 · see citing works
Klement, B. J., & Spooner, B. S. (1997) · Space technology and applications international forum (STAIF - 97) · doi:10.1063/1.52100 · Cited by 2 · see citing works
Ugele, B., & Lange, F. (2001) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1290/1071-2690(2001)037 · Cited by 24 · see citing works
Discussion: Development of Experimental Models for Ovarian Cancer
Hamilton, T. C., Becker, J. L., Kacinski, B. M., Nicosia, S. V., & Rodriquez, G. (2003) · Gynecologic Oncology · doi:10.1006/gyno.2002.6684 · Cited by 1 · see citing works
AYYASWAMY, P. S. (2023) · International Conference on Microgravity Fluid Physics and Heat Transfer · doi:10.1615/mfpht-1999.220 · Cited by 1 · see citing works
Marrero, B. (2010) · Digital Commons - University of South Florida (University of South Florida)
Elliott, N. T. (2010) · DukeSpace (Duke University)
Culturing mouse embryonic stem cells with microcarriers in rotary cell culture system
Wang, X., Wei, G., & Ma, X. (2010) · IEEE Annual Northeast Bioengineering Conference (NEBEC) · doi:10.1109/nebc.2010.5458161
Simulated microgravity model of ovarian tumor growth
Becker, J. L., Goodwin, T. J., Prewett, T. L., & Spaulding, G. F. (1993) · Space Programs and Technologies Conference · doi:10.2514/6.1993-4100
Francis, K. M., O’Connor, K. C., Prewett, T. L., Goodwin, T. J., & Spaulding, G. F. (1993) · Space Programs and Technologies Conference · doi:10.2514/6.1993-4099
Growth of human ovarian tumor cells in microgravity - Overview for ISS studies
Baker, T. L., Widen, R. H., Goodwin, T. J., Love, J., & Becker, J. L. (2001) · AIAA Conference and Exhibit on International Space Station Utilization · doi:10.2514/6.2001-5015
THREE-DIMENSIONAL ENDOTHELIAL-TUMOR EPITHELIAL CELL INTERACTIONS IN
Chopra, V., Dinh, T., & Hannigan, E. V. (1997)
Growth and Structure of Corneal Tissue in Simulated Microgravity
Francis, K. M., O’Connor, K. C., Blake, D. A., Caldwell, D. R., & Spaulding, G. F. (1995) · Springer eBooks · doi:10.1007/978-94-011-0437-1_153
Spatial and Temporal Microenvironmental Factors
(2010) · CRC Press eBooks · doi:10.1201/ebk1420073492-c6
Steins, A., Bijlsma, M. F., & van Laarhoven, H. W. M. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-811353-0.00054-3
Consolo, F., Fiore, G. B., Truscello, S., Caronna, M., Morbiducci, U., Montevecchi, F. M., & Redaelli, A. C. L. (2008) · Tissue Engineering Part A · doi:10.1089/ten.tea.2008.0213
5.3 Rotating-Wall Vessels for Cell Culture ☆
Qiu, Q.-Q., AYYASWAMY, P. S., & Ducheyne, P. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-803581-8.10185-7
Advances on Molecular and Biological Effects of Mammalian Cells in Microgravity Environment
Yu, Y., Shengwei, W. A. N. G., Zhaoxian, X. U., Mingjie, J. I. N., & Shulin, Y. A. N. G. (2018) · Chinese Journal of Space Science · doi:10.11728/cjss2018.06.891
- Authors
- Becker, J. L., Prewett, T. L., Spaulding, G. F., & Goodwin, T. J.
- Affiliations
- University of South Florida (US)
- Johnson Space Center (US)
- Corresponding author
- Becker, Jeanne L.
- ORCID
- Goodwin, Thomas J.
- Venue
- Journal of Cellular Biochemistry
- Publisher
- Wiley
- Published
- 1993-03
- Volume
- 51
- Issue
- 3
- Pages
- 283-289
- ISSN
- 0730-2312 (ISSN-L)
- 1097-4644
- OpenAlex
- W1969503471
- Cited by
- 91
- References
- 18
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 1 · 2022: 5 · 2021: 1 · 2020: 1 · 2018: 2 · 2017: 1 · 2016: 3 · 2014: 2 · 2013: 1
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cellular Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Ovarian cancer diagnosis and treatment
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- ovarian cancer, microcarrier beads, 3D cell culture, tumor cell differentiation, cell proliferation, multicellular aggregates, spheroids, mixed Mullerian tumor, tumor cell survival
- MeSH terms
- Biotechnology, Cell Differentiation, Cell Division, Female, Embryonic and Fetal Development, Humans, Ovarian Neoplasms, Rotation, Tumor Cells, Cultured, Culture Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
