Cited by 266TGF-β signaling in diseasesLiver physiology and pathologyT-cell and B-cell Immunology

Production of transforming growth factor beta by human peripheral blood monocytes and neutrophils

Grotendorst, Gary R., Smale, Georgeann, Pencev, Dobromir·1989·Journal of Cellular Physiology

Abstract

Human peripheral blood monocytes and neutrophils secrete TGF-beta. Activation of monocytes with LPS stimulates the secretion of TGF-beta; however, the production of TGF-beta by neutrophils was not altered by treatment with LPS. The secreted TGF-beta appears to be in a fully active form since acid treatment of the conditioned medium does not increase the amount of TGF-beta activity. TGF-beta 1 transcripts were detected at similar levels in both activated and nonactivated monocytes and neutrophils, suggesting that the increase in TGF-beta secretion after activation of monocytes is regulated by a posttranscriptional mechanism. Western blot analysis with anti-N-terminal TGF-beta 1 peptide antibodies indicate the leukocyte-derived TGF-beta is beta 1. In addition, TGF-beta 1 transcripts were detected in rat peritoneal macrophages and in a differentiating human hematopoietic tumor cell line (HEL). The ability of inflammatory cells such as monocytes and neutrophils to produce TGF-beta may play an important role in the function of these cells in wound repair, in the immune response, and in the pathogenesis of fibrotic diseases.

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Crossref · 10.1002/jcp.1041400226

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Authors
Grotendorst, G. R., Smale, G., & Pencev, D.
Affiliations
University of South Florida (US)
Corresponding author
Grotendorst, Gary R.
Venue
Journal of Cellular Physiology
Publisher
Wiley
Published
1989-08
Volume
140
Issue
2
Pages
396-402
ISSN
  • 0021-9541 (ISSN-L)
  • 1097-4652
OpenAlex
W2042965746
Cited by
262
References
34
Citations by year
2026: 1 · 2025: 2 · 2024: 6 · 2023: 7 · 2022: 11 · 2021: 6 · 2020: 5 · 2019: 2 · 2018: 6 · 2017: 7 · 2016: 7 · 2015: 7 · 2014: 6 · 2012: 7
FWCI
5.03
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Cellular Physiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › TGF-β signaling in diseases
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Keywords
TGF-beta, TGF-β1, monocytes, neutrophils, lipopolysaccharide, monocyte activation, post-transcriptional regulation, peritoneal macrophages, HEL cell line, wound healing, fibrotic diseases, immune response
MeSH terms
Animals, Antigen-Antibody Complex, Cell Line, Endotoxins, Gene Expression Regulation, Hematopoietic Stem Cells, Humans, Monocytes, Neutrophils, Rats, Inbred Strains, Immunoblotting, Transforming Growth Factors, Rats

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