Transforming growth factor beta 1-induced changes in cell migration, proliferation, and angiogenesis in the chicken chorioallantoic membrane.
Open accessAbstract
Application of TGF beta 1 (10-100 ng) to the chicken chorioallantoic membrane (CAM) for 72 h resulted in a dose-dependent, gross angiogenic response. The vascular effects induced by TGF beta 1 were qualitatively different than those induced by maximal doses of basic FGF (bFGF) (500 ng). While TGF beta 1 induced the formation of large blood vessels by 72 h, bFGF induced primarily small blood vessels. Histologic analysis revealed that TGF beta 1 stimulated pleiotropic cellular responses in the CAM. Increases in fibroblast and epithelial cell density in the area of TGF beta 1 delivery were observed as early as 4 h after TGF beta 1 treatment. By 8 h, these cell types also demonstrated altered morphology and marked inhibition of proliferation as evidenced by 3H-thymidine labeling. Thus, the TGF beta 1-stimulated accumulation of these cell types was the result of cellular chemotaxis from peripheral areas into the area of TGF beta 1 delivery. Microscopic angiogenesis in the form of capillary sprouts and increased endothelial cell density first became evident at 16 h. By 24 h, capillary cords appeared within the mesenchyme of the CAM, extending towards the point of TGF beta 1 delivery. 3H-thymidine labeling revealed that the growth of these capillary cords was due to endothelial cell proliferation. Finally, perivascular mononuclear inflammation did not become evident until 48 h of treatment, and its presence correlated spatially and temporally with the gross and histological remodelling of newly formed capillary cords into larger blood vessels. In summary, these data suggest that, in the chicken CAM, TGF beta 1 initiates a sequence of cellular responses that results in growth inhibition, cellular accumulation through migration, and microvascular angiogenesis.
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From TGFβ biology in cancer progression and immunotherapy (2021) · cites this paper as [273]
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- Authors
- Yang, E. Y., & Moses, H. L.
- Affiliations
- Vanderbilt University (US)
- ORCID
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1990-08-01
- Volume
- 111
- Issue
- 2
- Pages
- 731-741
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2116177
- OpenAlex
- W2041708400
- Cited by
- 464
- References
- 58
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 3 · 2022: 8 · 2021: 6 · 2020: 8 · 2019: 7 · 2018: 5 · 2017: 13 · 2016: 7 · 2015: 9 · 2014: 11 · 2013: 8 · 2012: 9
- FWCI
- 8.69
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r35ca042572-14)
- National Cancer Institute (CA42572)
- National Institute of General Medical Sciences (T32GM007347)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › TGF-β signaling in diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- TGF-β1, angiogenesis, chorioallantoic membrane, cell migration, cell proliferation, basic fibroblast growth factor, endothelial cell proliferation, fibroblast accumulation, chemotaxis, capillary sprouting, perivascular inflammation, vascular remodeling, chick embryo
- MeSH terms
- Allantois, Animals, Cell Division, Cell Movement, Chick Embryo, Chorion, Extraembryonic Membranes, Fibroblast Growth Factors, Kinetics, Neovascularization, Pathologic, Recombinant Proteins, Time Factors, Transforming Growth Factors, Macromolecular Substances
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