The effect of endosomal escape peptides on in vitro gene delivery of polyethylene glycol‐based vehicles
Abstract
BACKGROUND: With recent progress in gene therapy clinical trials, there is an even greater demand to advance the development of nonviral gene delivery vehicles. We have previously developed poly(ethylene glycol) (PEG)-based vehicles with transfection efficiency similar to polyethyleneimine and low cytotoxicity. It was hypothesized that conjugating endosomal escape peptides (EEPs) to PEG-based vehicles would further increase their transfection efficiency. The present study aimed to determine how two different EEPs, INF7 and H5WYG, which destabilize the endosomal membrane at different pHs, affect the efficiency of PEG-based vehicles. METHODS: INF7 and H5WYG were conjugated to PEG-tetraacrylate (PEG-TA) via a Michael-type addition at the desired molar ratios. The pH-dependent membrane lytic activity, transfection efficiency, particle size, zeta potential, and endosomal escape kinetic rate constants were determined. RESULTS: Fusogenic peptides, INF7 and H5WYG, showed pH-dependent membrane lytic activity when conjugated to PEG-TA. The highest membrane lytic activity of PEG-INF7 and PEG-H5WYG conjugates occurred at pH 5 and 5.5, respectively. Coupling one INF7 peptide to PEG-DNA binding peptide (DBP) vehicles increased the transfection efficiency ten-fold and showed greater transfection efficiency than PEG-DBP vehicles coupled with H5WYG peptide. Fitting a first-order kinetic model to the average intracellular pH of the vehicle/DNA particles over time determined that coupling EEPs to PEG-DBP vehicles increased the endosomal escape rate constant by two orders of magnitude. CONCLUSIONS: Endosomal escape is a key step in nonviral cellular trafficking and thus the transfection efficiency of nonviral vehicles can be increased by targeting release of DNA from the endosome with EEPs.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Membrane fusion peptides such as GALA peptides and acid-activatable melittin have been introduced into non-viral vectors to facilitate endosomal escape.
“In addition, inspired by the mechanism of viral infection, some membrane fusion peptides, such as GALA peptides and acid-activatable melittin, have been introduced into non-viral vectors to facilitate endosomal escape [184–186].”
From Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018) · cites this paper as [186]
Cites
Data Reduction and Error Analysis for the Physical Sciences
Bevington, P. R., Robinson, D. K., Blair, J. M., Mallinckrodt, A. J., & McKay, S. R. (1993) · Computers in Physics · doi:10.1063/1.4823194 · Cited by 14,992 · see citing works
Boussif, O., Lezoualc’h, F., Zanta, M. A., Djavaheri‐Mergny, M., Scherman, D., DEMENEIX, B. A., & Behr, J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.16.7297 · Cited by 6,256 · see citing works
Rejman, J., Oberle, V., Zuhorn, I. S., & Hoekstra, D. (2004) · Biochemical Journal · doi:10.1042/bj20031253 · Cited by 2,872 · see citing works
Synthetic DNA delivery systems
Luo, D., & Saltzman, W. M. (2000) · Nature Biotechnology · doi:10.1038/71889 · Cited by 1,625 · see citing works
THE STRUCTURE AND FUNCTION OF THE HEMAGGLUTININ MEMBRANE GLYCOPROTEIN OF INFLUENZA VIRUS
Wiley, D. C., & Skehel, J. J. (1987) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.56.070187.002053 · Cited by 1,413 · see citing works
Exploring polyethylenimine‐mediated DNA transfection and the proton sponge hypothesis
Akinc, A., Thomas, M., Klibanov, A. M., & Langer, R. (2004) · The Journal of Gene Medicine · doi:10.1002/jgm.696 · Cited by 1,339 · see citing works
Ogris, M., Brunner, S., Schüller, S., Kircheis, R., & Wagner, E. (1999) · Gene Therapy · doi:10.1038/sj.gt.3300900 · Cited by 1,178 · see citing works
Size-dependent DNA Mobility in Cytoplasm and Nucleus
Lukács, G. L., Haggie, P. M., Seksek, O., Lechardeur, D., Freedman, N. D., & Verkman, A. S. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.3.1625 · Cited by 752 · see citing works
High efficiency polyoma DNA transfection of chloroquine treated cells
Luthman, H., & Magnusson, G. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.5.1295 · Cited by 714 · see citing works
Plank, C., Oberhauser, B., Mechtler, K., Koch, C., & Wagner, E. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)99963-1 · Cited by 666 · see citing works
Wagner, E., Plank, C., Zatloukal, K., Cotten, M. L., & Birnstiel, M. L. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.17.7934 · Cited by 657 · see citing works
Mechanism of oligonucleotide release from cationic liposomes.
Zelphati, O., & Szoka, F. C. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.21.11493 · Cited by 634 · see citing works
Metabolic instability of plasmid DNA in the cytosol: a potential barrier to gene transfer
Lechardeur, D., Sohn, K.-J., Haardt, M., Joshi, P. B., Monck, M. A., Graham, R. W., Beatty, B., Squire, J. A., O'Brodovich, H. M., & Lukács, G. L. (1999) · Gene Therapy · doi:10.1038/sj.gt.3300867 · Cited by 632 · see citing works
Gene therapy clinical trials worldwide to 2007—an update
Edelstein, M. L., Abedi, M. R., & Wixon, J. (2007) · Journal of Gene Medicine · doi:10.1002/jgm.1100 · Cited by 629 · see citing works
Polyethylenimine-mediated gene delivery: a mechanistic study
Kichler, A., Leborgne, C., Coeytaux, E., & Danos, O. (2001) · The Journal of Gene Medicine · doi:10.1002/jgm.173 · Cited by 516 · see citing works
Wagner, E., Zatloukal, K., Cotten, M. L., Kirlappos, H., Mechtler, K., Curiel, D., & Birnstiel, M. L. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.13.6099 · Cited by 481 · see citing works
Barriers to Nonviral Gene Delivery
Wiethoff, C. M., & Middaugh, C. R. (2003) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.10286 · Cited by 480 · see citing works
Wyman, T. B., Nicol, F., Zelphati, O., Scaria, P. V., Plank, C., & Szoka, F. C. (1997) · Biochemistry · doi:10.1021/bi9618474 · Cited by 454 · see citing works
Adenovirus enhancement of transferrin-polylysine-mediated gene delivery.
Curiel, D. T., Agarwal, S., Wagner, E., & Cotten, M. L. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.19.8850 · Cited by 423 · see citing works
Non-viral gene delivery systems
Davis, M. E. (2002) · Current Opinion in Biotechnology · doi:10.1016/s0958-1669(02)00294-x · Cited by 419 · see citing works
Elbert, D. L., & Hubbell, J. A. (2001) · Biomacromolecules · doi:10.1021/bm0056299 · Cited by 409 · see citing works
Hindered diffusion of inert tracer particles in the cytoplasm of mouse 3T3 cells.
Luby‐Phelps, K., Castle, P. E., Taylor, D. L., & Lanni, F. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.14.4910 · Cited by 399 · see citing works
Bayer, N., Schober, D., Prchla, E., Murphy, R. F., Blaas, D., & Fuchs, R. (1998) · Journal of Virology · doi:10.1128/jvi.72.12.9645-9655.1998 · Cited by 329 · see citing works
Plank, C., Zatloukal, K., Cotten, M. L., Mechtler, K., & Wagner, E. (1992) · Bioconjugate Chemistry · doi:10.1021/bc00018a012 · Cited by 324 · see citing works
Cotten, M. L., Wagner, E., Zatloukal, K., Phillips, S., Curiel, D. T., & Birnstiel, M. L. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.13.6094 · Cited by 319 · see citing works
New Class of Polymers for the Delivery of Macromolecular Therapeutics
Gonzalez, H., Hwang, S., & Davis, M. E. (1999) · Bioconjugate Chemistry · doi:10.1021/bc990072j · Cited by 312 · see citing works
Determinants of the translational mobility of a small solute in cell cytoplasm.
Kao, H. P., Abney, J. R., & Verkman, A. S. (1993) · The Journal of Cell Biology · doi:10.1083/jcb.120.1.175 · Cited by 300 · see citing works
Kichler, A., Leborgne, C., März, J., Danos, O., & Bechinger, B. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0337677100 · Cited by 229 · see citing works
Hama, S., Akita, H., Ito, R., Mizuguchi, H., Hayakawa, T., & Harashima, H. (2005) · Molecular Therapy · doi:10.1016/j.ymthe.2005.10.007 · Cited by 211 · see citing works
Membrane Permeabilization and Efficient Gene Transfer by a Peptide Containing Several Histidines
Midoux, P., Kichler, A., Boutin, V., Maurizot, J., & Monsigny, M. L. P. (1998) · Bioconjugate Chemistry · doi:10.1021/bc9701611 · Cited by 209 · see citing works
Ogris, M., Steinlein, P., Carotta, S., Brunner, S., & Wagner, E. (2001) · AAPS PharmSci · doi:10.1208/ps030321 · Cited by 208 · see citing works
Towards Membrane Protein Design: pH-sensitive Topology of Histidine-containing Polypeptides
Bechinger, B. (1996) · Journal of Molecular Biology · doi:10.1006/jmbi.1996.0614 · Cited by 201 · see citing works
Application of membrane-active peptides for drug and gene delivery across cellular membranes
Plank, C., Zauner, W., & Wagner, E. (1998) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(98)00005-2 · Cited by 181 · see citing works
Morral, N., O’Neal, W. K., Zhou, H., Langston, C., & Beaudet, A. L. (1997) · Human Gene Therapy · doi:10.1089/hum.1997.8.10-1275 · Cited by 170 · see citing works
Intact Microtubules Support Adenovirus and Herpes Simplex Virus Infections
Mabit, H., Nakano, M. Y., Prank, U., Saam, B., Döhner, K., Sodeik, B., & Greber, U. F. (2002) · Journal of Virology · doi:10.1128/jvi.76.19.9962-9971.2002 · Cited by 164 · see citing works
Akinc, A., & Langer, R. (2002) · Biotechnology and Bioengineering · doi:10.1002/bit.20215 · Cited by 143 · see citing works
Miyazawa, N., Crystal, R. G., & Leopold, P. L. (2001) · Journal of Virology · doi:10.1128/jvi.75.3.1387-1400.2001 · Cited by 142 · see citing works
On the Kinetics of Polyplex Endocytic Trafficking: Implications for Gene Delivery Vector Design
Forrest, M. L., & Pack, D. W. (2002) · Molecular Therapy · doi:10.1006/mthe.2002.0631 · Cited by 135 · see citing works
Cationic amphipathic histidine-rich peptides for gene delivery
Kichler, A., Mason, A. J., & Bechinger, B. (2006) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2006.02.005 · Cited by 127 · see citing works
Quantitative Analysis of Synthetic Gene Delivery Vector Design Properties
Varga, C. M., Hong, K., & Lauffenburger, D. A. (2001) · Molecular Therapy · doi:10.1006/mthe.2001.0475 · Cited by 120 · see citing works
Kinetics of endosome acidification in mutant and wild-type Chinese hamster ovary cells.
Yamashiro, D. J., & Maxfield, F. R. (1987) · The Journal of Cell Biology · doi:10.1083/jcb.105.6.2713 · Cited by 110 · see citing works
Synthesis and Characterization of Degradable p(HEMA) Microgels: Use of Acid‐Labile Crosslinkers
Bulmuş, V., Chan, Y., Nguyen, Q., & Tran, H. L. (2007) · Macromolecular Bioscience · doi:10.1002/mabi.200600258 · Cited by 89 · see citing works
Murata, M., Takahashi, S., Kagiwada, S., Suzuki, A., & Ohnishi, S. (1992) · Biochemistry · doi:10.1021/bi00122a013 · Cited by 89 · see citing works
Evaluation of adjuvants that enhance the effectiveness of antisense oligodeoxynucleotides
Hughes, J. A., Aronsohn, A. I., Avrutskaya, A. V., & Juliano, R. L. (1996) · Pharmaceutical Research · doi:10.1023/a:1016044609972 · Cited by 80 · see citing works
Song, W., Kong, H.-L., Traktman, P., & Crystal, R. G. (1997) · Human Gene Therapy · doi:10.1089/hum.1997.8.10-1207 · Cited by 71 · see citing works
Kulkarni, R. P., Mishra, S., Fraser, S. E., & Davis, M. E. (2005) · Bioconjugate Chemistry · doi:10.1021/bc050081u · Cited by 67 · see citing works
Glycerol Enhancement of Ligand-Polylysine/DNA Transfection
Zauner, W., Kichler, A., Schmidt, W., Sinski, A., & Wagner, E. (1996) · BioTechniques · doi:10.2144/96205rr04 · Cited by 67 · see citing works
Lappalainen, K., Miettinen, R. A., Kellokoski, J. K., Jääskeläinen, I., & Syrjänen, S. (1997) · Journal of Histochemistry & Cytochemistry · doi:10.1177/002215549704500211 · Cited by 47 · see citing works
Zinc improves gene transfer mediated by DNA/cationic polymer complexes
Pichon, C., Guérin, B., Réfrégiers, M., Gonçalves, C., Vigny, P., & Midoux, P. (2002) · The Journal of Gene Medicine · doi:10.1002/jgm.303 · Cited by 42 · see citing works
van Rossenberg, S. M. W., Sliedregt, K., Meeuwenoord, N. J., van Berkel, T. J. C., BOOM, J. H. V., van der MAREL, G. A., & Biessen, E. A. L. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m203510200 · Cited by 41 · see citing works
Membrane permeabilization by α-helical peptides: a flow cytometry study
Midoux, P., Mayer, R., & Monsigny, M. L. P. (1995) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(95)00163-w · Cited by 40 · see citing works
Moore, N. M., Barbour, T. R., & Sakiyama‐Elbert, S. E. (2007) · Molecular Pharmaceutics · doi:10.1021/mp700072n · Cited by 32 · see citing works
Warren, J. C., & Cassimeris, L. (2007) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.20215 · Cited by 27 · see citing works
Development of novel poly(ethylene glycol)‐based vehicles for gene delivery
Schmieder, A. H., Grabski, L. E., Moore, N. M., Dempsey, L. A., & Sakiyama‐Elbert, S. E. (2006) · Biotechnology and Bioengineering · doi:10.1002/bit.21199 · Cited by 19 · see citing works
Modification of the N-terminus of membrane fusion-active peptides blocks the fusion activity
Murata, M., Kagiwada, S., Hishida, R., Ishiguro, R., Ohnishi, S., & Takahashi, S. (1991) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(91)91925-3 · Cited by 16 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Designing multifunctional quantum dots for bioimaging, detection, and drug delivery
Zrazhevskiy, P., Sena, M., & Gao, X. (2010) · Chemical Society Reviews · doi:10.1039/b915139g · Cited by 999 · see citing works
Midoux, P., Pichon, C., Yaouanc, J., & Jaffrès, P. (2009) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.2009.00288.x · Cited by 549 · see citing works
Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities
Li, L., Hu, S., & Chen, X. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.04.031 · Cited by 415 · see citing works
Evans, B. C., Nelson, C. E., Yu, S. S., Beavers, K. R., Kim, A. J., Li, H., Nelson, H. M., Giorgio, T. D., & Duvall, C. L. (2013) · Journal of Visualized Experiments · doi:10.3791/50166 · Cited by 356 · see citing works
Cell-Specific Delivery of Diverse Cargos by Bacteriophage MS2 Virus-like Particles
Ashley, C. E., Carnes, E. C., Phillips, G. K., Durfee, P. N., Buley, M. D., Lino, C. A., Padilla, D. P., Phillips, B., Carter, M. B., Willman, C. L., et al. (2011) · ACS Nano · doi:10.1021/nn201397z · Cited by 350 · see citing works
Intracellular delivery of nanomaterials: How to catch endosomal escape in the act
Martens, T., Remaut, K., Demeester, J., Smedt, S. C. D., & Braeckmans, K. (2014) · Nano Today · doi:10.1016/j.nantod.2014.04.011 · Cited by 341 · see citing works
The Multirole of Liposomes in Therapy and Prevention of Infectious Diseases
Nisini, R., Poerio, N., Mariotti, S., Santis, F. D., & Fraziano, M. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.00155 · Cited by 299 · see citing works
Ashley, C. E., Carnes, E. C., Epler, K. E., Padilla, D. P., Phillips, G. K., Castillo, R., Wilkinson, D., Wilkinson, B., Burgard, C., Kalinich, R. M., et al. (2012) · ACS Nano · doi:10.1021/nn204102q · Cited by 241 · see citing works
Engineered nanomaterial uptake and tissue distribution: from cell to organism
Huwyler, J., Kettiger, H., Schipanski, A., & Wick, P. (2013) · International Journal of Nanomedicine · doi:10.2147/ijn.s49770 · Cited by 212 · see citing works
Wang, P., Zheng, X., Guo, Q., Yang, P., Pang, X., Qian, K., Lü, W., Zhang, Q., & Jiang, X. (2018) · Journal of Controlled Release · doi:10.1016/j.jconrel.2018.04.034 · Cited by 162 · see citing works
Polyion complex (PIC) particles: Preparation and biomedical applications
Insua, I., Wilkinson, A., & Fernández‐Trillo, F. (2016) · European Polymer Journal · doi:10.1016/j.eurpolymj.2016.06.003 · Cited by 138 · see citing works
Cai, X., Jin, R., Wang, J., Yue, D., Jiang, Q., Wu, Y., & Gu, Z. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b11545 · Cited by 113 · see citing works
Advancing Nonviral Gene Delivery: Lipid- and Surfactant-Based Nanoparticle Design Strategies
Donkuru, M., Badea, I., Wettig, S. D., Verrall, R. E., Elsabahy, M., & Földvári, M. (2010) · Nanomedicine · doi:10.2217/nnm.10.80 · Cited by 103 · see citing works
Endosome‐disruptive peptides for improving cytosolic delivery of bioactive macromolecules
Nakase, I., Kobayashi, S., & Futaki, S. (2010) · Biopolymers · doi:10.1002/bip.21487 · Cited by 98 · see citing works
Effect of innate glutathione levels on activity of redox-responsive gene delivery vectors
Manickam, D. S., Li, J., Putt, D. A., Zhou, Q.-H., Wu, C., Lash, L. H., & Oupický, D. (2009) · Journal of Controlled Release · doi:10.1016/j.jconrel.2009.08.022 · Cited by 97 · see citing works
Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide
Wang, H., Zhong, C.-Y., Wu, J., Huang, Y., & Liu, C.-B. (2009) · Journal of Controlled Release · doi:10.1016/j.jconrel.2009.12.003 · Cited by 79 · see citing works
Moore, N. M., Sheppard, C. L., & Sakiyama‐Elbert, S. E. (2008) · Acta Biomaterialia · doi:10.1016/j.actbio.2008.09.009 · Cited by 63 · see citing works
Biological activities of histidine-rich peptides; merging biotechnology and nanomedicine
Ferrer‐Miralles, N., Corchero, J. L., Kumar, P., Cedano, J. A., Gupta, K. C., Villaverde, A., & Vázquez, E. (2011) · Microbial Cell Factories · doi:10.1186/1475-2859-10-101 · Cited by 58 · see citing works
Advances in Polymeric and Inorganic Vectors for Nonviral Nucleic Acid Delivery
Sunshine, J. C., Bishop, C. J., & Green, J. J. (2011) · Therapeutic Delivery · doi:10.4155/tde.11.14 · Cited by 55 · see citing works
Nogueira-Librelotto, D. R., Mitjans, M., Infante, M. R., & Vinardell, M. P. (2011) · Acta Biomaterialia · doi:10.1016/j.actbio.2011.03.017 · Cited by 53 · see citing works
Functionalization of silica nanoparticles for nucleic acid delivery
Kamegawa, R., Naito, M., & Miyata, K. (2018) · Nano Research · doi:10.1007/s12274-018-2116-7 · Cited by 53 · see citing works
Design, engineering and preparation of a multi-domain fusion vector for gene delivery
Sadeghian, F., Hosseinkhani, S., Alizadeh, A., & Hatefi, A. (2012) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2012.01.062 · Cited by 49 · see citing works
Sun, Y., Liu, H., Cheng, L., Zhu, S., Cai, C., Yang, T., Yang, L., & Ding, P. (2017) · Polymer International · doi:10.1002/pi.5490 · Cited by 48 · see citing works
Jin, G., Koo, H., Nam, K., Kim, H., Lee, S., Park, J., & Lee, Y. (2010) · Polymer · doi:10.1016/j.polymer.2010.10.066 · Cited by 45 · see citing works
Epler, K. E., Padilla, D., Phillips, G. K., Crowder, P., Castillo, R., Wilkinson, D., Wilkinson, B., Burgard, C., Kalinich, R. M., Townson, J. L., et al. (2012) · Advanced Healthcare Materials · doi:10.1002/adhm.201200022 · Cited by 43 · see citing works
Fan, Y., Li, C., Cao, H., Li, F., & Chen, D. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.02.038 · Cited by 42 · see citing works
Zhang, B., & Mallapragada, S. K. (2010) · Acta Biomaterialia · doi:10.1016/j.actbio.2010.11.033 · Cited by 41 · see citing works
Multifunctional Hyperbranched Glycoconjugated Polymers Based on Natural Aminoglycosides
Chen, M., Hu, M. L., Wang, D., Wang, G., Zhu, X., Yan, D., & Sun, J. (2012) · Bioconjugate Chemistry · doi:10.1021/bc300016b · Cited by 40 · see citing works
Recent Advances in the Rational Design of silica-based Nanoparticles for Gene Therapy
Niu, Y., Popat, A., Yu, M., Karmakar, S., Gu, W., & Yu, C. (2012) · Therapeutic Delivery · doi:10.4155/tde.12.98 · Cited by 38 · see citing works
Where in the Cell Is our Cargo? Methods Currently Used To Study Intracellular Cytosolic Localisation
Méndez‐Ardoy, A., Lostalé‐Seijo, I., & Montenegro, J. (2018) · ChemBioChem · doi:10.1002/cbic.201800390 · Cited by 36 · see citing works
Biocompatible fluorinated poly(β-amino ester)s for safe and efficient gene therapy
Gong, J., Wang, Y., Xing, L., Cui, P., Qiao, J.-B., He, Y., & Jiang, H. (2017) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2017.11.015 · Cited by 35 · see citing works
He, M., Potuck, A., Kohn, J. C., Fung, K., Reinhart‐King, C. A., & Chu, C. (2015) · Biomacromolecules · doi:10.1021/acs.biomac.5b01449 · Cited by 33 · see citing works
Surface Modification of Nonviral Nanocarriers for Enhanced Gene Delivery
Fortier, C., Durocher, Y., & Crescenzo, G. D. (2013) · Nanomedicine · doi:10.2217/nnm.13.194 · Cited by 31 · see citing works
Ahmad, A., & Khan, J. M. (2022) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2022.103786 · Cited by 31 · see citing works
Nogueira-Librelotto, D. R., Mitjans, M., Morán, M. C., Pérez, L., & Vinardell, M. P. (2011) · Amino Acids · doi:10.1007/s00726-011-1176-8 · Cited by 28 · see citing works
Exploring the role of peptides in polymer-based gene delivery
Sun, Y., Yang, Z. L., Wang, C., Yang, T., Cai, C., Zhao, X., Yang, L., & Ding, P. (2017) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.07.043 · Cited by 28 · see citing works
Nogueira-Librelotto, D. R., Scheeren, L. E., Vinardell, M. P., Mitjans, M., & Rolim, C. M. B. (2016) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2016.08.014 · Cited by 27 · see citing works
What can we learn from virus in designing nonviral gene vectors
Xu, C.-H., Sui, M., Tang, J., & Shen, Y. (2011) · Chinese Journal of Polymer Science · doi:10.1007/s10118-011-1047-5 · Cited by 23 · see citing works
Nogueira-Librelotto, D. R., Scheeren, L. E., Vinardell, M. P., Mitjans, M., Infante, M. R., & Rolim, C. M. B. (2015) · Materials Science and Engineering C · doi:10.1016/j.msec.2015.07.036 · Cited by 22 · see citing works
Plasmid Transfection in Mammalian Cells Spatiotemporally Tracked by a Gold Nanoparticle
Muroski, M. E., Carnevale, K. J. F., Riskowski, R. A., & Strouse, G. F. (2014) · ACS Nano · doi:10.1021/nn5060305 · Cited by 21 · see citing works
Fan, Y., Li, F., & Chen, D. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2014.05.054 · Cited by 20 · see citing works
Sung, M. S., Mun, J.-Y., Kwon, O., Kwon, K., & Oh, D. (2013) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2013.06.058 · Cited by 19 · see citing works
Cian, M., & Helen, O. (2013) · InTech eBooks · doi:10.5772/54271 · Cited by 17 · see citing works
Drouin, M., Cayer, M., & Jung, D. (2010) · Virology · doi:10.1016/j.virol.2010.03.003 · Cited by 17 · see citing works
Modeling nanoparticle uptake and intracellular distribution using stochastic process algebras
Dobay, M. P., Alberola, A. P., Mendoza, E., & Rädler, J. O. (2012) · Journal of Nanoparticle Research · doi:10.1007/s11051-012-0821-9 · Cited by 15 · see citing works
Asseline, U., Gonçalves, C., Pichon, C., & Midoux, P. (2014) · The Journal of Gene Medicine · doi:10.1002/jgm.2773 · Cited by 15 · see citing works
The Discovery of the Buffer Capacity of Various Types of Polyelectrolyte Microcapsules
Dubrovskii, A. V., Kim, A. L., Musin, E. V., Ramazanov, B. R., & Tikhonenko, S. A. (2021) · Polymers · doi:10.3390/polym13224026 · Cited by 13 · see citing works
Polli, J. R., & Balthasar, J. P. (2022) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.2c00152 · Cited by 12 · see citing works
Zhang, Y., Jiang, Q., Bi, B., Xu, L., Liu, J., Zhuo, R., & Jiang, X. (2018) · Journal of Materials Chemistry B · doi:10.1039/c7tb02170d · Cited by 12 · see citing works
Mejia, F., Khan, S., Omstead, D. T., Minetos, C., & Bilgiçer, B. (2022) · Nanoscale · doi:10.1039/d1nr05357d · Cited by 12 · see citing works
Lin, N., Zheng, W., Li, L., Liu, H., Wang, T., Wang, P., & Ma, X. (2013) · Targeted Oncology · doi:10.1007/s11523-013-0282-9 · Cited by 9 · see citing works
Quantitative cellular uptake of double fluorescent core-shelled model submicronic particles
Leclerc, L., Boudard, D., Pourchez, J., Forest, V., Marmuse, L., Louis, C., Bin, V., Palle, S., Grosseau, P., Bernache‐Assollant, D., et al. (2012) · Journal of Nanoparticle Research · doi:10.1007/s11051-012-1221-x · Cited by 8 · see citing works
Advanced Polymers for Nonviral Gene Delivery
Zhou, D., Zeng, M., Gao, Y., Sigen, A., Lyu, J., & Wang, W. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-814470-1.00010-1 · Cited by 8 · see citing works
A novel telecommunications-based approach to HIV modeling and simulation
Sharp, A., Pannier, A. K., Wysocki, B. J., & Wysocki, T. A. (2012) · Nano Communication Networks · doi:10.1016/j.nancom.2012.01.003 · Cited by 8 · see citing works
Improved DNA delivery using invasive E. coli DH10B in human cells by modified bactofection method
Sultana, A., & Tiash, S. (2021) · Journal of Controlled Release · doi:10.1016/j.jconrel.2021.02.004 · Cited by 6 · see citing works
New biocompatible polymeric micelles designed for efficient intracellular uptake and delivery
Maria, F. D., Blasi, L., Quarta, A., Bergamini, G., Barbarella, G., Giorgini, L., & Benaglia, M. (2015) · Journal of Materials Chemistry B · doi:10.1039/c5tb01631b · Cited by 4 · see citing works
Polidori, I., Weber, L. I., Keim, S., To, D., Hartl, M., & Bernkop‐Schnürch, A. (2026) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2026.126554 · Cited by 2 · see citing works
A NOVEL TELECOMMUNICATIONS-BASED APPROACH TO MATHEMATICAL MODELING OF HIV INFECTION
Sharp, A. (2011) · Lincoln (University of Nebraska) · Cited by 1 · see citing works
Nanosized Vectors for Transfection Assembled from Peptides and Nucleic Acids
Bechinger, B. (2013) · doi:10.1002/9781118592403.ch9 · Cited by 1 · see citing works
DNA Nanoparticles for Non-viral Gene Therapy: Mechanistic Studies and Targeting
Sun, W. (2012) · OhioLink ETD Center (Ohio Library and Information Network)
Anti-GD3 antibodies are targeting molecules for delivery of siRNA to melanoma
Wu, M. W.-Y. (2010) · QSpace (Queen's University Library)
Synthetic delivery systems that control release of anticancer agents from early endosomes
Yang, N. (2013) · KU ScholarWorks (The University of Kansas)
Peng, J. W., Wang, Y., Wu, T., Tan, L., & Tang, M. (2021) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2021.102503
- Authors
- Moore, N. M., Sheppard, C. L., Barbour, T. R., & Sakiyama‐Elbert, S. E.
- Affiliations
- Washington University in St. Louis (US)
- Corresponding author
- Sakiyama‐Elbert, Shelly E.
- Venue
- The Journal of Gene Medicine
- Publisher
- Wiley
- Published
- 2008-07-21
- Volume
- 10
- Issue
- 10
- Pages
- 1134-1149
- ISSN
- 1099-498X (ISSN-L)
- 1521-2254
- OpenAlex
- W1975366433
- Cited by
- 63
- References
- 55
- Citations by year
- 2026: 1 · 2022: 3 · 2021: 3 · 2019: 1 · 2018: 6 · 2017: 3 · 2016: 3 · 2015: 3 · 2014: 4 · 2013: 8 · 2012: 10
- FWCI
- 2.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Gene Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Keywords
- non-viral gene delivery, transfection efficiency, DNA-binding peptides, intracellular pH
- MeSH terms
- Animals, Cell Survival, Cricetulus, Genetic Vectors, Cricetinae, Hemolysis, Humans, Models, Biological, Peptides, Polyethylene Glycols, Endosomes, Transfection, Genetic Therapy, CHO Cells, Gene Transfer Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
