Secretion of TNF-α by alveolar macrophages in response to Candida albicans mannan
Abstract
Resident alveolar macrophages (AMø) were tested for their ability to respond to Candida albicans mannan. AMø were found to produce tumor necrosis factor alpha (TNF-α) in vitro in response to mannan stimulation. TNF-α secretion was measured using ELISA and L929B cellular cytotoxicity assays. Cytotoxicity was neutralized in parallel L929B cell cultures by the addition of rabbit anti-TNF-α antibody. Mannan preparations were found to be free of contaminating LPS by Limulus assay. When AMø were cultivated for 18 h at 37°, 67 μg of mannan stimulated the secretion of approximately 207 U/ml of TNF-α. By comparison, AMø treated with 6.7 μg of LPS secreted approximately 257 U/ml of TNF-α. Optimal TNF-α production occurred between 9 and 18 h after mannan stimulation. Disparate mechanisms for stimulation of TNF-α secretion were suggested by differential sugar blockade of LPS- and mannan-induced TNF-α secretion. The addition of 2% D-mannose or 2% alpha-methyl-D-mannoside to AMø cultures blocked mannan-but not LPS-stimulated TNF-α secretion. Furthermore, the addition of rabbit anti-mannan antibody to mannan-coated plastic culture dishes prevented TNF-α secretion by the mannan-sensitive RAW 264.7 cell line. Moreover, the data suggest that C. albicans mannan stimulated AMø to secrete TNF-α by an LPS-independent receptor mechanism which may also function as a mannose receptor. J. Leukoc. Biol. 55: 161–168; 1994.
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Mannans from C. albicans cell wall have the ability to stimulate cytokine production.
“Mannans from C. albicans cell wall have the ability to stimulate cytokine production126,127, and induce dendritic cell maturation128.”
From Fungal Hsp90: a biological transistor that tunes cellular outputs to thermal inputs (2012) · cites this paper as [127]
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- Authors
- Garner, R. E., Rubanowice, K., Sawyer, R. T., & Hudson, J. A.
- Affiliations
- Mercer University (US)
- Corresponding author
- Garner, Ronald E
- Venue
- Journal of Leukocyte Biology
- Publisher
- Oxford University Press (OUP)
- Published
- 1994-02-01
- Volume
- 55
- Issue
- 2
- Pages
- 161-168
- ISSN
- 0741-5400 (ISSN-L)
- 1938-3673
- OpenAlex
- W2182298035
- Cited by
- 102
- References
- 30
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 2 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 4
- FWCI
- 3.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Leukocyte Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Keywords
- alveolar macrophages, TNF-alpha secretion, CD206, alpha-methyl-D-mannoside, D-mannose, anti-mannan antibodies, RAW 264.7 macrophages, fungal cell wall polysaccharides
- MeSH terms
- Animals, Biological Assay, Candida, Candida albicans, Cells, Cultured, Kinetics, Lipopolysaccharides, Male, Mannans, Mice, Inbred BALB C, Mice, Inbred DBA, Saccharomyces cerevisiae, Time Factors, Tumor Necrosis Factor-alpha, Macrophages, Alveolar, Mice
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