Cited by 102Immune Response and InflammationAntifungal resistance and susceptibilityGlycosylation and Glycoproteins Research

Secretion of TNF-α by alveolar macrophages in response to Candida albicans mannan

Garner, Ronald E, Rubanowice, Katherine, Sawyer, Richard T, Hudson, John A·1994·Journal of Leukocyte Biology

Abstract

Resident alveolar macrophages (AMø) were tested for their ability to respond to Candida albicans mannan. AMø were found to produce tumor necrosis factor alpha (TNF-α) in vitro in response to mannan stimulation. TNF-α secretion was measured using ELISA and L929B cellular cytotoxicity assays. Cytotoxicity was neutralized in parallel L929B cell cultures by the addition of rabbit anti-TNF-α antibody. Mannan preparations were found to be free of contaminating LPS by Limulus assay. When AMø were cultivated for 18 h at 37°, 67 μg of mannan stimulated the secretion of approximately 207 U/ml of TNF-α. By comparison, AMø treated with 6.7 μg of LPS secreted approximately 257 U/ml of TNF-α. Optimal TNF-α production occurred between 9 and 18 h after mannan stimulation. Disparate mechanisms for stimulation of TNF-α secretion were suggested by differential sugar blockade of LPS- and mannan-induced TNF-α secretion. The addition of 2% D-mannose or 2% alpha-methyl-D-mannoside to AMø cultures blocked mannan-but not LPS-stimulated TNF-α secretion. Furthermore, the addition of rabbit anti-mannan antibody to mannan-coated plastic culture dishes prevented TNF-α secretion by the mannan-sensitive RAW 264.7 cell line. Moreover, the data suggest that C. albicans mannan stimulated AMø to secrete TNF-α by an LPS-independent receptor mechanism which may also function as a mannose receptor. J. Leukoc. Biol. 55: 161–168; 1994.

Integrity signals

Registry

Crossref · 10.1002/jlb.55.2.161

Cites

31 of 31 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Garner, R. E., Rubanowice, K., Sawyer, R. T., & Hudson, J. A.
Affiliations
Mercer University (US)
Corresponding author
Garner, Ronald E
Venue
Journal of Leukocyte Biology
Published
1994-02-01
Volume
55
Issue
2
Pages
161-168
ISSN
  • 0741-5400 (ISSN-L)
  • 1938-3673
OpenAlex
W2182298035
Cited by
102
References
30
Citations by year
2023: 1 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 2 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 4
FWCI
3.45
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Leukocyte Biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
Keywords
alveolar macrophages, TNF-alpha secretion, CD206, alpha-methyl-D-mannoside, D-mannose, anti-mannan antibodies, RAW 264.7 macrophages, fungal cell wall polysaccharides
MeSH terms
Animals, Biological Assay, Candida, Candida albicans, Cells, Cultured, Kinetics, Lipopolysaccharides, Male, Mannans, Mice, Inbred BALB C, Mice, Inbred DBA, Saccharomyces cerevisiae, Time Factors, Tumor Necrosis Factor-alpha, Macrophages, Alveolar, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review