The DOI matches its Crossref record; 67 of the 67 references it cites were found in a registry or an index.
Chronic morphine induces premature mitosis of proliferating cells in the adult mouse subgranular zone
Abstract
The birth of cells with neurogenic potential in the adult brain is assessed commonly by detection of exogenous S phase markers, such as bromodeoxyuridine (BrdU). Analysis of other phases of the cell cycle, however, can provide insight into how external factors, such as opiates, influence the cycling of newly born cells. To this end, we examined the expression of two endogenous cell cycle markers in relation to BrdU: proliferating cell nuclear antigen (PCNA) and phosphorylated histone H3 (pHisH3). Two hours after one intraperitoneal BrdU injection, BrdU‐, PCNA‐, and pHisH3‐immunoreactive (IR) cells exhibited similar distribution in the adult mouse subgranular zone (SGZ). Quantitative analysis within the SGZ revealed a relative abundance of cells labeled for PCNA > BrdU >> pHisH3. Similar to our reports in rat SGZ, chronic morphine treatment decreased BrdU‐ and PCNA‐IR cells in mouse SGZ by 28 and 38%, respectively. We also show that pHisH3‐IR cells are influenced by chronic morphine to a greater extent (58% decrease) than are BrdU‐ or PCNA‐IR cells. Cell cycle phase analysis of SGZ BrdU‐IR cells using triple labeling for BrdU, PCNA, and pHisH3 revealed premature mitosis in chronic morphine‐treated mice. These results suggest that morphine‐treated mice have a shorter Gap2/mitosis (G 2/M) phase when compared to sham‐treated mice. These findings demonstrate the power of using a combination of exogenous and endogenous cell cycle markers and nuclear morphology to track proliferating cells through different phases of the cell cycle and to reveal the regulation of cell cycle phase by chronic morphine. © 2004 Wiley‐Liss, Inc.
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“Critically, not only do opioids interfere with memory acquisition and retrieval, but they also interfere with hippocampal neurogenesis, where chronic administration of morphine leads to reduced hippocampal neurogenesis and cell proliferation (Eisch et al., 2000;Kahn et al., 2005;Mandyam et al., 2004).”
From A review of the relation between dissociation, memory, executive functioning and social cognition in military members and civilians with neuropsychiatric conditions (2016) · cites this paper as Mandyam et al., 2004
In the citing paper
“Critically, not only do opioids interfere with memory acquisition and retrieval, but they also interfere with hippocampal neurogenesis, where chronic administration of morphine leads to reduced hippocampal neurogenesis and cell proliferation (Eisch et al., 2000;Kahn et al., 2005;Mandyam et al., 2004).”
From A review of the relation between dissociation, memory, executive functioning and social cognition in military members and civilians with neuropsychiatric conditions (2016) · cites this paper as Mandyam et al., 2004
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Research works printed but not linked: 3
- Eisch AJ, Use of endogenous cell cycle markers to examine adult mammalian neurogenesis: Basic and clinical implications, Nova Science
- 10.1002/(SICI)1097-4644(1998)72:30/31 <1::AID-JCB2>3.0.CO;2-E · DOI
- Norris RD, 2002, Use of endogenous cell cycle markers as a novel measure of cell proliferation in the adult hippocampus, Society for Neuroscience, 126, 2
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Items 101–115 of 115
Kreisler, A. D., Terranova, M. J., Somkuwar, S. S., Purohit, D. C., Wang, S., Head, B. P., & Mandyam, C. D. (2020) · Brain Structure and Function · doi:10.1007/s00429-020-02059-w · Cited by 5
Self-citationEffects of Ethanol on the Regulation of Cell Cycle in Neural Stem Cells
Zawada, W. M., & Das, M. (2006) · Oxford University Press eBooks · doi:10.1093/acprof:oso/9780195183139.003.0012 · Cited by 5
Khan, M. S., Trenet, W., Xing, N., Sibley, B. D., Abbas, M., al‐Rashida, M., Rauf, K., & Mandyam, C. D. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21124411 · Cited by 4
Self-citationHu, J., Cui, F., & Zhang, X. (2020) · Open Life Sciences · doi:10.1515/biol-2020-0006 · Cited by 3
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Self-citationBrain Regeneration - in vitro and in vivo studies of exercise-related effects on brain plasticity
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DynamicContributionofNestin-ExpressingStemCellsto AdultNeurogenesis
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Vinícius, M., & Nascimento, L. (2013) · Americanae (AECID Library)
The Effect of Opiates on the Developing Cerebral Cortex
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Yi, M., Liu, Q., Yang, J., Wang, R., He, J., Zhang, H., & Zhang, H. (2026) · Scientific Reports · doi:10.1038/s41598-026-63436-9
Sengupta, R., & Meucci, O. (2009) · Springer eBooks · doi:10.1007/978-1-4419-0793-6_17
Metadata
- Authors
- Mandyam, C. D., Norris, R. D., & Eisch, A. J.
- Affiliations
- The University of Texas Southwestern Medical Center (US)
- Corresponding author
- Eisch, Amelia J.
- Venue
- Journal of Neuroscience Research
- Publisher
- Wiley
- Published
- 2004-05-03
- Volume
- 76
- Issue
- 6
- Pages
- 783-794
- ISSN
- 0360-4012 (ISSN-L)
- 1097-4547
- OpenAlex
- W2064038456
- Cited by
- 116
- References
- 67
- Citations by year
- 2026: 1 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 3 · 2018: 5 · 2017: 3 · 2016: 6 · 2015: 2 · 2014: 13 · 2013: 5 · 2012: 6
- FWCI
- 2.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neuroscience Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute on Aging (P30AG012300, AG 12300)
- National Institute on Drug Abuse (DA-07290)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Pharmacological Receptor Mechanisms and Effects
- Keywords
- chronic morphine, subgranular zone, adult neurogenesis, premature mitosis, cell cycle phases, proliferating cell nuclear antigen, phosphorylated histone H3, bromodeoxyuridine, G2/M phase, opiates
- MeSH terms
- Animals, Bromodeoxyuridine, Cell Cycle, Cell Differentiation, Drug Administration Schedule, Hippocampus, Histones, Immunohistochemistry, Male, Mice, Inbred C57BL, Morphine, Neurons, Phosphorylation, Stem Cells, Biomarkers, Proliferating Cell Nuclear Antigen, Mice
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