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Cited by 2,853Neurogenesis and neuroplasticity mechanismsMemory and Neural MechanismsNeuroscience and Neuropharmacology Research

Functional neurogenesis in the adult hippocampus

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van Praag, Henriette, Schinder, Alejandro F., Christie, Brian R., Toni, Nicolas, Palmer, Theo D., Gage, Fred H.·2002·Nature
DOI 10.1038/4151030aPMC PMC9284568
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Abstract

There is extensive evidence indicating that new neurons are generated in the dentate gyrus of the adult mammalian hippocampus, a region of the brain that is important for learning and memory. However, it is not known whether these new neurons become functional, as the methods used to study adult neurogenesis are limited to fixed tissue. We use here a retroviral vector expressing green fluorescent protein that only labels dividing cells, and that can be visualized in live hippocampal slices. We report that newly generated cells in the adult mouse hippocampus have neuronal morphology and can display passive membrane properties, action potentials and functional synaptic inputs similar to those found in mature dentate granule cells. Our findings demonstrate that newly generated cells mature into functional neurons in the adult mammalian brain.

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  • Doublecortin (DCX) is a protein expressed in developing immature neurons, but silenced as those neurons mature.

    “doublecortin (DCX), a protein expressed in developing immature neurons, but silenced as those neurons mature37”

    From Therapeutically viable generation of neurons with antisense oligonucleotide suppression of PTB (2021) · cites this paper as [37]

    The Methods (page unavailable) list the neuronal markers examined but do not mention doublecortin or DCX. The paper discusses maturation of newly generated neurons and possible shutdown of GFP expression (Abstract, page unavailable), not whether DCX expression is silenced as neurons mature. Searches for “doublecortin DCX” and immature-neuron marker expression found no passage addressing the claim. (LLM, conf=0.76)

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Authors
van Praag, H., Schinder, A. F., Christie, B. R., Toni, N., Palmer, T. D., & Gage, F. H.
Affiliations
Salk Institute for Biological Studies (US)
ORCID
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2002-02
Volume
415
Issue
6875
Pages
1030-1034
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/4151030a
PMC
PMC9284568
OpenAlex
W1963940970
Cited by
2,853
References
3
Citations by year
2026: 28 · 2025: 52 · 2024: 48 · 2023: 45 · 2022: 55 · 2021: 84 · 2020: 105 · 2019: 90 · 2018: 79 · 2017: 126 · 2016: 130 · 2015: 120 · 2014: 144 · 2013: 142 · 2012: 151
FWCI
35.04
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Funders
National Institute of Mental Health (R01MH090258)
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
adult neurogenesis, dentate gyrus, hippocampus, dentate gyrus granule cells, neuronal maturation, retroviral labeling, live imaging, neuronal morphology, action potential, synaptic input, passive membrane properties
MeSH terms
Aging, Animals, Cell Differentiation, Cell Division, Female, Genetic Vectors, Luminescent Proteins, Membrane Potentials, Mice, Inbred C57BL, Neural Pathways, Neurons, Synapses, Dentate Gyrus, Green Fluorescent Proteins, Mice

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