Cited by 43Nanoparticle-Based Drug DeliveryBarrier Structure and Function StudiesRNA Interference and Gene Delivery

Neuroprotection Against Degeneration of SK-N-MC Cells Using Neuron Growth Factor-Encapsulated Liposomes with Surface Cereport and Transferrin

Kuo, Yung-Chih, Chou, Pei-Ru·2014·Journal of Pharmaceutical Sciences

Abstract

Liposomes with Cereport (RMP-7) and transferrin (Tf) (RMP-7/Tf/liposomes) were employed to target the blood-brain barrier (BBB) and to inhibit the degeneration of neurons insulted with fibrillar β-amyloid peptide 1-42 (Aβ1-42). Neuron growth factor (NGF)-encapsulated RMP-7/Tf/liposomes (RMP-7/Tf/NGF-liposomes) were used to permeate a monolayer of human brain-microvascular endothelial cells (HBMECs) regulated by human astrocytes (HAs) and to treat Aβ1-42 -attacked SK-N-MC cells. An increase in RMT-7 concentration increased the particle size, zeta potential, propidium iodide (PI) permeability, and NGF permeability, but decreased the cross-linking efficiency of RMT-7, viability of HBMECs and HAs, and transendothelial electrical resistance (TEER). In addition, an increase in Tf concentration enhanced the particle size, viability of HBMECs, HAs, and SK-N-MC cells, PI permeability, and NGF permeability, but reduced the zeta potential, cross-linking efficiency of RMT-7 and Tf, and TEER. RMP-7/Tf/NGF-liposomes can transport NGF across the BBB and improve the neuroprotection for Alzheimer's disease therapy in preclinical trials.

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Crossref · 10.1002/jps.24081

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  • Encapsulation of curcumin and NGF in combination led to synergistic effect and showed clinical application in mitigating Aβ-induced neurotoxicity.

    “Interestingly, the encapsulation of curcumin and NGF in combination led to a synergistic effect and showed a clinical application in mitigating Aβ-induced neurotoxicity.109,110”

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Authors
Kuo, Y.-C., & Chou, P.-R.
Affiliations
National Chung Cheng University (TW)
Corresponding author
Kuo, Yung-Chih
Venue
Journal of Pharmaceutical Sciences
Publisher
Elsevier BV
Published
2014-08
Volume
103
Issue
8
Pages
2484-2497
ISSN
0022-3549 (ISSN-L)
OpenAlex
W2047044627
Cited by
43
References
74
Citations by year
2026: 1 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 4 · 2021: 1 · 2020: 3 · 2019: 5 · 2018: 3 · 2017: 8 · 2016: 4 · 2015: 5 · 2014: 1
FWCI
2.27
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Pharmaceutical Sciences (publishedVersion)
  • PubMed (publishedVersion)
Funders
Ministry of science and technology (NSC 101-2221-E-194 -051 -MY3)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Keywords
liposomes, transferrin, blood-brain barrier, amyloid-beta 42, neuroprotection, Alzheimer's disease, SK-N-MC cells, brain microvascular endothelial cells, astrocytes, transendothelial electrical resistance, liposome surface modification, drug delivery
MeSH terms
Alzheimer Disease, Astrocytes, Bradykinin, Brain, Cell Line, Cell Survival, Humans, Liposomes, Nerve Growth Factors, Peptide Fragments, Transferrin, Amyloid beta-Peptides, Drug Delivery Systems, Neuroprotective Agents, Endothelial Cells

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