Neuroprotection Against Degeneration of SK-N-MC Cells Using Neuron Growth Factor-Encapsulated Liposomes with Surface Cereport and Transferrin
Abstract
Liposomes with Cereport (RMP-7) and transferrin (Tf) (RMP-7/Tf/liposomes) were employed to target the blood-brain barrier (BBB) and to inhibit the degeneration of neurons insulted with fibrillar β-amyloid peptide 1-42 (Aβ1-42). Neuron growth factor (NGF)-encapsulated RMP-7/Tf/liposomes (RMP-7/Tf/NGF-liposomes) were used to permeate a monolayer of human brain-microvascular endothelial cells (HBMECs) regulated by human astrocytes (HAs) and to treat Aβ1-42 -attacked SK-N-MC cells. An increase in RMT-7 concentration increased the particle size, zeta potential, propidium iodide (PI) permeability, and NGF permeability, but decreased the cross-linking efficiency of RMT-7, viability of HBMECs and HAs, and transendothelial electrical resistance (TEER). In addition, an increase in Tf concentration enhanced the particle size, viability of HBMECs, HAs, and SK-N-MC cells, PI permeability, and NGF permeability, but reduced the zeta potential, cross-linking efficiency of RMT-7 and Tf, and TEER. RMP-7/Tf/NGF-liposomes can transport NGF across the BBB and improve the neuroprotection for Alzheimer's disease therapy in preclinical trials.
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Encapsulation of curcumin and NGF in combination led to synergistic effect and showed clinical application in mitigating Aβ-induced neurotoxicity.
“Interestingly, the encapsulation of curcumin and NGF in combination led to a synergistic effect and showed a clinical application in mitigating Aβ-induced neurotoxicity.109,110”
From Targeted therapy in chronic diseases using nanomaterial-based drug delivery vehicles (2019) · cites this paper as [110]
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Self-citation
- Authors
- Kuo, Y.-C., & Chou, P.-R.
- Affiliations
- National Chung Cheng University (TW)
- Corresponding author
- Kuo, Yung-Chih
- ORCID
- Kuo, Yung-Chih
- Venue
- Journal of Pharmaceutical Sciences
- Publisher
- Elsevier BV
- Published
- 2014-08
- Volume
- 103
- Issue
- 8
- Pages
- 2484-2497
- ISSN
- 0022-3549 (ISSN-L)
- OpenAlex
- W2047044627
- Cited by
- 43
- References
- 74
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 4 · 2021: 1 · 2020: 3 · 2019: 5 · 2018: 3 · 2017: 8 · 2016: 4 · 2015: 5 · 2014: 1
- FWCI
- 2.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Pharmaceutical Sciences (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of science and technology (NSC 101-2221-E-194 -051 -MY3)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- liposomes, transferrin, blood-brain barrier, amyloid-beta 42, neuroprotection, Alzheimer's disease, SK-N-MC cells, brain microvascular endothelial cells, astrocytes, transendothelial electrical resistance, liposome surface modification, drug delivery
- MeSH terms
- Alzheimer Disease, Astrocytes, Bradykinin, Brain, Cell Line, Cell Survival, Humans, Liposomes, Nerve Growth Factors, Peptide Fragments, Transferrin, Amyloid beta-Peptides, Drug Delivery Systems, Neuroprotective Agents, Endothelial Cells
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