Cited by 122Nanoparticle-Based Drug DeliveryBarrier Structure and Function StudiesRNA Interference and Gene Delivery

Uptake and permeability studies of BBB-targeting immunoliposomes using the hCMEC/D3 cell line.

Markoutsa, Eleni, Pampalakis, Georgios, Niarakis, Anna, Romero, Ignacio A., Weksler, Babette, Couraud, Pierre-Olivier, Antimisiaris, Sophia G.·2011·European Journal of Pharmaceutics and Biopharmaceutics

Abstract

The targeting potential of OX-26-decorated immunoliposomes was investigated, using the human brain endothelial cell line hCMEC/D3 as a model of the blood-brain barrier (BBB). Immuno-nanoliposomes were prepared by the biotin/streptavidin ligation strategy, and their uptake by hCMEC/D3 cells and permeability through cell monolayers was studied. In order to elucidate the mechanisms of uptake, pH-sensitive fluorescence signal of HPTS was used, while transport was measured using double labeled immunoliposomes (with aqueous and lipid membrane fluorescent tags). PEGylated and non-specific-IgG-decorated liposomes were studied under identical conditions, as controls. CHO-K1 cells (which do not overexpress the transferrin receptor) were studied in some cases for comparative purposes. Experimental results reveal that hCMEC/D3 cells are good models for in vitro screening of BBB-targeting nanoparticulate drug delivery systems. Uptake and transcytosis of immunoliposome-associated dyes by cell monolayers was substantially higher compared to those of control liposomes. HPTS-entrapping OX-26-immunoliposome uptake indicated lysosomal localization and receptor-mediated mechanism. The ratio of aqueous/lipid label transport is affected by pre-incubation with antibody, or use of high lipid doses, suggesting that vesicles are transported intact after lysosome saturation. Co-decoration with a second ligand slightly decreases OX-26-decorated vesicle uptake, but not transcytosis, proving that the biotin-streptavidin technique can be applied for the generation of dual-targeting nanoliposomes.

Integrity signals

Registry

Crossref · 10.1016/j.ejpb.2010.11.015

Cites

44 of 44 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Physiochemical properties of drug-loaded nanocarriers such as hydrophilicity, surface charge, and targeting ligands determine adsorption on brain capillary endothelial cells.

    “The physiochemical properties of drug loaded nanocarrier like hydrophilicity, surface charge and targeting ligands of nano carrier is the deciding factor for the adsorption on the brain capillary endothelial cells”

    From Nanotechnology: A Promising Approach for Delivery of Neuroprotective Drugs (2020) · cites this paper as Markoutsa et al., 2011

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Markoutsa, E., Pampalakis, G., Niarakis, A., Romero, I. A., Weksler, B., Couraud, P.-O., & Antimisiaris, S. G.
Affiliations
  • University of Patras (GR)
  • The Open University (GB)
  • Cornell University (US)
  • Inserm (FR)
Corresponding author
Antimisiaris, Sophia G.
Venue
European Journal of Pharmaceutics and Biopharmaceutics
Publisher
Elsevier BV
Published
2011-02
Volume
77
Issue
2
Pages
265-274
ISSN
0939-6411 (ISSN-L)
OpenAlex
W2035669214
Cited by
122
References
44
Citations by year
2026: 2 · 2025: 3 · 2024: 4 · 2023: 6 · 2022: 7 · 2021: 7 · 2020: 3 · 2019: 8 · 2018: 7 · 2017: 10 · 2016: 8 · 2015: 7 · 2014: 17 · 2013: 12 · 2012: 5
FWCI
4.22
Type
journal article
Language
en
Open access
closed
Locations
  • European Journal of Pharmaceutics and Biopharmaceutics (publishedVersion)
  • PubMed (publishedVersion)
  • Open Research Online - ORO (The Open University) (acceptedVersion)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Keywords
immunoliposomes, blood-brain barrier targeting, hCMEC/D3 cells, transferrin receptor, receptor-mediated uptake, transcytosis, lysosomal uptake, PEGylated liposomes, HPTS, brain drug delivery
MeSH terms
Animals, Antibodies, Monoclonal, Biological Transport, Blood-Brain Barrier, Cell Line, Cell Survival, Cells, Cultured, Humans, Immunoglobulin G, Liposomes, Particle Size, Permeability, Receptors, Transferrin, Transferrin, Endothelial Cells, Mice, Nanoparticles, Chemical Phenomena, Transcytosis

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review