Cited by 56Nanoparticle-Based Drug DeliveryBarrier Structure and Function StudiesRNA Interference and Gene Delivery

Rescuing apoptotic neurons in Alzheimer’s disease using wheat germ agglutinin-conjugated and cardiolipin-conjugated liposomes with encapsulated nerve growth factor and curcumin

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Kuo, Yung-Chih, Lin, Ching-Chun·2015·International Journal of Nanomedicine

Abstract

Liposomes with cardiolipin (CL) and wheat germ agglutinin (WGA) were developed to permeate the blood-brain barrier and treat Alzheimer's disease. WGA-conjugated and CL-incorporated liposomes (WGA-CL-liposomes) were used to transport nerve growth factor (NGF) and curcumin (CUR) across a monolayer of human brain-microvascular endothelial cells regulated by human astrocytes and to protect SK-N-MC cells against apoptosis induced by β-amyloid1-42 (Aβ(1-42)) fibrils. An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute zeta potential value, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ(1-42), but decreased the atomic ratio of nitrogen to phosphorus and release of CUR. In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability of NGF and CUR across the blood-brain barrier, but reduced the absolute zeta potential value and biocompatibility. WGA-CL-liposomes carrying NGF and CUR could be promising colloidal delivery carriers for future clinical application in targeting the blood-brain barrier and inhibiting neurotoxicity.

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  • Encapsulation of curcumin and NGF in combination led to synergistic effect and showed clinical application in mitigating Aβ-induced neurotoxicity.

    “Interestingly, the encapsulation of curcumin and NGF in combination led to a synergistic effect and showed a clinical application in mitigating Aβ-induced neurotoxicity.109,110”

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Authors
Kuo, Y.-C., & Lin, C.-C.
Affiliations
National Chung Cheng University (TW)
Corresponding author
Kuo, Yung-Chih
Venue
International Journal of Nanomedicine
Published
2015-04
Volume
10
Pages
2653
ISSN
  • 1176-9114 (ISSN-L)
  • 1178-2013
OpenAlex
W2015976719
Cited by
56
References
66
Citations by year
2026: 4 · 2024: 3 · 2023: 6 · 2022: 6 · 2021: 2 · 2020: 7 · 2019: 3 · 2018: 7 · 2017: 10 · 2016: 5 · 2015: 3
FWCI
3.01
Type
journal article
Language
en
Open access
Locations
  • International Journal of Nanomedicine (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Keywords
Alzheimer's disease, liposomes, wheat germ agglutinin, cardiolipin, nerve growth factor, curcumin, blood-brain barrier permeability, neuronal apoptosis, brain microvascular endothelial cells, SK-N-MC cells, drug carriers, liposome encapsulation
MeSH terms
Alzheimer Disease, Cardiolipins, Curcumin, Humans, Liposomes, Neurons, Wheat Germ Agglutinins, Apoptosis, Nerve Growth Factor

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