Reference database of Raman spectra of biological molecules
Open accessAbstract
Abstract Raman spectra of biological materials are very complex, because they consist of signals from all molecules present in cells. In order to obtain chemical information from these spectra, it is necessary to know the Raman patterns of the possible components of a cell. In this paper, we present a collection of Raman spectra of biomolecules that can serve as references for the interpretation of Raman spectra of biological materials. We included the most important components present in a cell: (1) DNA and RNA bases (adenine, cytosine, guanine, thymine and uracil), (2) amino acids (glycine, L ‐alanine, L ‐valine, L ‐serine, L ‐glutamic acid, L ‐arginine, L ‐phenylalanine, L ‐tyrosine, L ‐tryptophan, L ‐histidine, L ‐proline), (3) fatty acids and fats (lauric acid, myristic acid, palmitic acid, stearic acid, 12‐methyltetradecanoic acid, 13‐methylmyristic acid, 14‐methylpentadecanoic acid, 14‐methylhexadecanoic acid, 15‐methylpalmitic acid, oleic acid, vaccenic acid, glycerol, triolein, trilinolein, trilinolenin), (4) saccharides (β‐ D ‐glucose, lactose, cellulose, D ‐(+)‐dextrose, D ‐(+)‐trehalose, amylose, amylopectine, D ‐(+)‐mannose, D ‐(+)‐fucose, D ‐(−)‐arabinose, D ‐(+)‐xylose, D ‐(−)‐fructose, D ‐(+)‐galactosamine, N ‐acetyl‐ D ‐glucosamine, chitin), (5) primary metabolites (citric acid, succinic acid, fumarate, malic acid, pyruvate, phosphoenolpyruvate, coenzyme A, acetyl coenzyme A, acetoacetate, D ‐fructose‐6‐phosphate) and (6) others (β‐carotene, ascorbic acid, riboflavin, glutathione). Examples of Raman spectra of bacteria and fungal spores are shown, together with band assignments to the reference products. Copyright © 2007 John Wiley & Sons, Ltd.
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The three microalgae are potentially a good biodiesel feedstock.
“However, the smaller intensity of these bands in comparison to the saturated fatty acids at ~1440 cm -1 indicate that these three microalgae are rich with saturated fatty acids, and confirms the potential of these microalgae to be a good source for biodiesel.[34,36,38].”
From Biomolecular transitions and lipid accumulation in green microalgae monitored by FTIR and Raman analysis (2020) · cites this paper as [34]
Because the unsaturation bands were weaker than the saturated fatty acid band near 1440 cm-1, the three microalgae appear rich in saturated fatty acids.
“However, the smaller intensity of these bands in comparison to the saturated fatty acids at ~1440 cm -1 indicate that these three microalgae are rich with saturated fatty acids, and confirms the potential of these microalgae to be a good source for biodiesel.[34,36,38].”
From Biomolecular transitions and lipid accumulation in green microalgae monitored by FTIR and Raman analysis (2020) · cites this paper as [34]
Lipid unsaturation gives characteristic vibrations at about 3008 cm-1 (=C-H), 1655 cm-1 (C=C), and 1270 cm-1 (=C-H).
“The characteristic vibrations of lipid unsaturation are (=C-H) at ~3008 cm -1 , (C=C) at ~1655 cm -1 and (=C-H) at ~1270 cm -1 [21,34,50].”
From Biomolecular transitions and lipid accumulation in green microalgae monitored by FTIR and Raman analysis (2020) · cites this paper as [34]
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- Authors
- De Gelder, J., De Gussem, K., Vandenabeele, P., & Moens, L.
- Affiliations
- Ghent University (BE)
- Corresponding author
- Vandenabeele, Peter
- ORCID
- Vandenabeele, Peter
- Venue
- Journal of Raman Spectroscopy
- Publisher
- Wiley
- Published
- 2007-04-11
- Volume
- 38
- Issue
- 9
- Pages
- 1133-1147
- ISSN
- 0377-0486 (ISSN-L)
- 1097-4555
- OpenAlex
- W1963638325
- Cited by
- 1,425
- References
- 22
- Citations by year
- 2026: 81 · 2025: 104 · 2024: 91 · 2023: 89 · 2022: 126 · 2021: 102 · 2020: 107 · 2019: 99 · 2018: 88 · 2017: 83 · 2016: 77 · 2015: 74 · 2014: 67 · 2013: 59 · 2012: 47
- FWCI
- 14.78
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Raman Spectroscopy (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
- Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Gold and Silver Nanoparticles Synthesis and Applications
- Keywords
- Raman spectra, biomolecules, Raman band assignment, DNA bases, amino acids, fatty acids, carbohydrates, primary metabolites, bacteria, fungal spores, cell composition, spectral interpretation, chitin, coenzyme A, pyruvate, N-acetylglucosamine, glycerol, trehalose, cellulose
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