The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Intra‐laboratory validation of microplate methods for total phenolic content and antioxidant activity on polyphenolic extracts, and comparison with conventional spectrophotometric methods
Abstract
BACKGROUND: Total phenolic content (TPC) and antioxidant activity (AA) assays in microplates save resources and time, therefore they can be useful to overcome the fact that the conventional methods are time‐consuming, labour intensive and use large amounts of reagents. An intra‐laboratory validation of the Folin–Ciocalteu microplate method to measure TPC and the 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) microplate method to measure AA was performed and compared with conventional spectrophotometric methods. RESULTS: To compare the TPC methods, the confidence intervals of a linear regression were used. In the range of 10–70 mg L−1 of gallic acid equivalents (GAE), both methods were equivalent. To compare the AA methodologies, the F‐test and t‐test were used in a range from 220 to 320 µmol L−1 of Trolox equivalents. Both methods had homogeneous variances, and the means were not significantively different. The limits of detection and quantification for the TPC microplate method were 0.74 and 2.24 mg L−1 GAE and for the DPPH 12.07 and 36.58 µmol L−1 of Trolox equivalents. The relative standard deviation of the repeatability and reproducibility for both microplate methods were ≤6.1%. The accuracy ranged from 88% to 100%. CONCLUSION: The microplate and the conventional methods are equals in a 95% confidence level. © 2014 Society of Chemical Industry
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Cited by
Alexandre, E. M. C., Silva, S., Santos, S. A. O., Silvestre, A. J. D., Duarte, M. F., Saraiva, J. A., & Pintado, M. (2019) · Food Research International · doi:10.1016/j.foodres.2018.08.044 · Cited by 217
Valente, I. M., Maia, M. R. G., Malushi, N., Oliveira, H. M., Papa, L., Rodrigues, J. A., Fonseca, A. J. M., & Cabrita, A. R. J. (2018) · Phytochemistry · doi:10.1016/j.phytochem.2018.05.011 · Cited by 106
Pagels, F., Amaro, H. M., Tavares, T. G., Amil, B. F., & Guedes, A. C. (2022) · Life · doi:10.3390/life12111901 · Cited by 35
Ferreira, S. M., Gomes, S. M., & Santos, L. (2023) · Molecules · doi:10.3390/molecules28052037 · Cited by 34
Brito, I., Ferreira, S. M., & Santos, L. (2023) · Cosmetics · doi:10.3390/cosmetics10050140 · Cited by 15
Metadata
- Authors
- Bobo‐García, G., Davidov‐Pardo, G., Arroqui, C., Vírseda, P., Marín‐Arroyo, M. R., & Navarro, M.
- Venue
- Journal of the Science of Food and Agriculture
- Publisher
- Wiley
- Published
- 2014-05-21
- Volume
- 95
- Issue
- 1
- Pages
- 204-209
- ISSN
- 0022-5142 (ISSN-L)
- 1097-0010
- OpenAlex
- W1552103058
- Cited by
- 322
- References
- 14
- FWCI
- 3.79
- Type
- journal article
- Language
- en
- Open access
- closed
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- Bobo‐García, G., Davidov‐Pardo, G., Arroqui, C., Vírseda, P., Marín‐Arroyo, M. R., & Navarro, M. (2015). Intra‐laboratory validation of microplate methods for total phenolic content and antioxidant activity on polyphenolic extracts, and comparison with conventional spectrophotometric methods. Journal of the Science of Food and Agriculture. https://doi.org/10.1002/jsfa.6706
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