Combination of solvent extraction with deep eutectic solvent based dispersive liquid–liquid microextraction for the analysis of aflatoxin M1 in cheese samples using response surface methodology optimization
Abstract
A new extraction procedure based on combination of a solvent extraction and deep eutectic solvent based dispersive liquid–liquid microextraction has been introduced for the extraction of aflatoxin M 1 from cheese samples. In this method, acetonitrile, deionized water, and n‐hexane are added onto the sample and vortexed. Owning to different affinities of the substances in cheese toward the mentioned solvents, an efficient and selective extraction of the analyte is done in the acetonitrile phase. After centrifugation, the acetonitrile phase is removed and mixed with a new hydrophobic deep eutectic solvent prepared from N,N‐diethanol ammonium chloride and carvacrol as an extraction solvent. The mixture is injected into deionized water, and a cloudy solution is obtained. Eventually, an aliquot of the organic phase is injected into high‐performance liquid chromatography‐fluorescence detection. After optimizing the effective parameters with the response surface methodology and a quadratic model, limits of detection and quantification were 0.74 and 2.56 ng/kg, respectively. The obtained extraction recovery and enrichment factor were 94% and 94, respectively. Also, intra‐ (n = 6) and interday (n = 4) precisions were less than or equal to 8.6% at a concentration of 5 ng/kg. The suggested method was applied to determine aflatoxin M 1 in different cheese samples.
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This DES can interact with the polar groups and the aromatic rings of aflatoxin.
“This DES can interact with the polar groups and the aromatic rings of aflatoxin.”
From Use of green alternative solvents in dispersive liquid‐liquid microextraction: A review (2022) · cites this paper as [76]
In 2021, Masrouri et al. combined diethyl amine chloride and carvacrol to obtain a DES for determination of aflatoxin M1 in cheese samples.
“In this year 2021, Masrouri et al. [76] combined diethyl amine chloride and carvacrol to obtain a DES for the determination of aflatoxin M1 in cheese samples.”
From Use of green alternative solvents in dispersive liquid‐liquid microextraction: A review (2022) · cites this paper as [76]
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- Authors
- Masrouri, M., Afshar Mogaddam, M. R., Farajzadeh, M. A., Nemati, M., & Lotfipour, F.
- Affiliations
- Tabriz University of Medical Sciences (IR)
- Food and Drug Safety Research Center Tabriz University of Medical Sciences Tabriz Iran
- University of Tabriz (IR)
- Corresponding author
- Lotfipour, Farzaneh
- Venue
- Journal of Separation Science
- Publisher
- Wiley
- Published
- 2021-02-17
- Volume
- 44
- Issue
- 7
- Pages
- 1501-1509
- ISSN
- 1615-9306 (ISSN-L)
- 1615-9314
- OpenAlex
- W3121943342
- Cited by
- 18
- References
- 33
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 3 · 2023: 5 · 2021: 4
- FWCI
- 1.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Separation Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
- Life Sciences › Agricultural and Biological Sciences › Food Science › Pesticide Residue Analysis and Safety
- Keywords
- aflatoxin M1, cheese, deep eutectic solvents, dispersive liquid-liquid microextraction, solvent extraction, mycotoxin analysis, carvacrol, ammonium chloride, response surface methodology, food safety
- MeSH terms
- Cymenes, Ethanol, Ammonium Chloride, Cheese, Solvents, Surface Properties, Aflatoxin M1, Liquid Phase Microextraction
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