Sugary Kefir Strain Lactobacillus mali APS1 Ameliorated Hepatic Steatosis by Regulation of SIRT‐1/Nrf‐2 and Gut Microbiota in Rats
Abstract
Scope: Non‐alcoholic fatty liver disease (NAFLD) is a common disease that is concomitant with obesity, resulting in increased mortality. To date, the efficiency of NAFLD treatment still needs to be improved. Therefore, we aimed to evaluate the effect of Lactobacillus mali APS1, which was isolated from sugary kefir, on hepatic steatosis in rats fed a high‐fat diet (HFD). Methods and results: Sprague Dawley rats were fed a control diet, a HFD with saline, and a HFD with APS1 intervention by gavage daily for 12 weeks. The results showed that APS1 significantly reduced body weight and body weight gain in HFD‐fed rats. APS1 reduced hepatic lipid accumulation by regulating SIRT‐1/PGC‐1α/SREBP‐1 expression. Moreover, APS1 increased hepatic antioxidant activity by modulating Nrf‐2/HO‐1 expression. Notably, APS1 manipulated the gut microbiota, resulting in increasing proportions of the phylum Bacteroidetes/Firmicutes and reducing the abundance of specific NAFLD‐associated bacteria. Conclusion: These results suggested that APS1 ameliorated hepatic steatosis by modulating lipid metabolism and antioxidant activity via manipulating specific NAFLD‐associated gut microbiota in vivo.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Lactobacillus mali APS1 acted through changes in intestinal microbiota composition, reducing the proportion of bacteria associated with non-alcoholic liver diseases and regulating lipid metabolism and oxidative stress response, leading to suppression of hepatic steatosis progression.
“This strain acted through changes in intestinal microbiota composition, reducing the proportion of bacteria associated with non-alcoholic liver diseases and regulating lipid metabolism and oxidative stress response, which leads to suppression of hepatic steatosis progression (29).”
From Kefir and Intestinal Microbiota Modulation: Implications in Human Health (2021) · cites this paper as [29]
Lactobacillus mali APS1 is a strain isolated from kefir grain.
“Lactobacillus mali APS1 is a strain isolated from kefir grain that may be useful in the treatment of diabetes.”
From Kefir and Intestinal Microbiota Modulation: Implications in Human Health (2021) · cites this paper as [29]
In mice consuming a high-fat diet, administration of Lactobacillus mali APS1 reduced serum glucose and HOMA index and increased levels of glucagon-like peptide (GLP-1) and butyrate.
“In a study (Table 1) with mice consuming a high-fat diet, the administration of this strain was able to reduce serum glucose and HOMA index, increasing the levels of glucagon-like peptide (GLP-1) and butyrate (29).”
From Kefir and Intestinal Microbiota Modulation: Implications in Human Health (2021) · cites this paper as [29]
In rats fed a high-fat diet, Lactobacillus mali APS1 isolated from sugary kefir grains significantly reduced weight, weight gain, hepatic lipid accumulation, and serum levels of AST and ALT.
“The effect of Lactobacillus mali APS1, isolated from sugary kefir grains, was also tested in the fatty liver of rat fed with a high-fat diet, showing a significant reduction in weight, weight gain, hepatic lipid accumulation, and serum levels of AST and ALT.”
From Kefir and Intestinal Microbiota Modulation: Implications in Human Health (2021) · cites this paper as [29]
Cites
Microbiota and SCFA in lean and overweight healthy subjects
Schwiertz, A., Taras, D., Schäfer, K., Beijer, S., Bos, N. A., Donus, C., & Hardt, P. D. (2010) · Obesity · doi:10.1038/oby.2009.167 · Cited by 2,457 · see citing works
Sirtuins as regulators of metabolism and healthspan
Houtkooper, R. H., Pirinen, E., & Auwerx, J. (2012) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3293 · Cited by 2,008 · see citing works
Adiponectin and AdipoR1 Regulate PGC-1α and Mitochondria by Ca2+ and AMPK/SIRT1
Iwabu, M., Yamauchi, T., Okada-Iwabu, M., Sato, K., Nakagawa, T., Funata, M., Yamaguchi, M., Namiki, S., Nakayama, R., Tabata, M., et al. (2010) · Nature · doi:10.1038/nature08991 · Cited by 971 · see citing works
A Taxonomic Signature of Obesity in the Microbiome? Getting to the Guts of the Matter
Finucane, M. M., Sharpton, T. J., Laurent, T. J., & Pollard, K. S. (2014) · PLoS ONE · doi:10.1371/journal.pone.0084689 · Cited by 321 · see citing works
Hepatic triacylglycerol accumulation and insulin resistance
Nagle, C. A., Klett, E. L., & Coleman, R. A. (2009) · Journal of Lipid Research · doi:10.1194/jlr.r800053-jlr200 · Cited by 272 · see citing works
Non-alcoholic fatty liver and the gut microbiota
Bashiardes, S., Shapiro, H., Rozin, S., Shibolet, O., & Elinav, E. (2016) · Molecular Metabolism · doi:10.1016/j.molmet.2016.06.003 · Cited by 241 · see citing works
Yoo, S., Kim, Y., Park, D., Jung, U., Jeon, S., Ahn, Y., Huh, C., McGregor, R., & Choi, M. S. (2013) · Obesity · doi:10.1002/oby.20428 · Cited by 230 · see citing works
Anti-Obesity Effect of Lactobacillus gasseri BNR17 in High-Sucrose Diet-Induced Obese Mice
Kang, J.-H., Yun, S.-I., Park, M.-H., Park, J.-H., Jeong, S.-Y., & Park, H.-O. (2013) · PLoS ONE · doi:10.1371/journal.pone.0054617 · Cited by 230 · see citing works
Kefir prevented excess fat accumulation in diet-induced obese mice
Choi, J.-W., Kang, H. W., Lim, W.-C., Kim, M.-K., Lee, I.-Y., & Cho, H.-Y. (2017) · Bioscience, Biotechnology, and Biochemistry · doi:10.1080/09168451.2016.1258984 · Cited by 43 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Kefir and Intestinal Microbiota Modulation: Implications in Human Health
Peluzio, M. D. C. G., Dias, M. D. M. E., Martinez, J. A., & Milagro, F. I. (2021) · Frontiers in Nutrition · doi:10.3389/fnut.2021.638740 · Cited by 92 · see citing works
In Kim, H., Kim, J.-K., Kim, J.-Y., Jang, S.-E., Han, M. J., & Kim, D.-H. (2019) · Nutrition Research · doi:10.1016/j.nutres.2019.03.008 · Cited by 82 · see citing works
- Authors
- Chen, Y., Lin, Y., Lin, J., Yang, N., & Chen, M.
- Affiliations
- National Taiwan University (TW)
- Taiwan Livestock Research Institute (TW)
- Open University of Kaohsiung (TW)
- Corresponding author
- Yang, Ning‐Sun
- Chen, Ming‐Ju
- Venue
- Molecular Nutrition & Food Research
- Publisher
- Wiley
- Published
- 2018-04
- Volume
- 62
- Issue
- 8
- Pages
- e1700903-e1700903
- ISSN
- 1613-4125 (ISSN-L)
- 1613-4133
- OpenAlex
- W2793300620
- Cited by
- 77
- References
- 9
- Citations by year
- 2026: 4 · 2025: 9 · 2024: 12 · 2023: 4 · 2022: 6 · 2021: 13 · 2020: 15 · 2019: 10 · 2018: 3
- FWCI
- 8.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Nutrition & Food Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Science and Technology, Taiwan (MOST 104‐2313‐B‐002‐043‐MY3)
- Topics
- Health Sciences › Medicine › Epidemiology › Liver Disease Diagnosis and Treatment
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- hepatic steatosis, non-alcoholic fatty liver disease, high-fat diet, gut microbiota, SIRT1, Nrf2, PGC-1α, SREBP1, heme oxygenase-1, hepatic lipid accumulation, lipid metabolism, antioxidant activity, Firmicutes/Bacteroidetes ratio, probiotics
- MeSH terms
- Gastrointestinal Microbiome, Kefir, Animals, Anti-Inflammatory Agents, Non-Steroidal, Antioxidants, Gene Expression Regulation, Lactobacillus, Lipotropic Agents, Liver, Male, Obesity, Random Allocation, Biomarkers, Weight Gain, Rats, Sprague-Dawley, Oxidative Stress, Anti-Obesity Agents, GA-Binding Protein Transcription Factor, Sirtuin 1, Prebiotics, Diet, High-Fat, Non-alcoholic Fatty Liver Disease
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
