Influence of resistance exercise intensity and metabolic stress on anabolic signaling and expression of myogenic genes in skeletal muscle
Abstract
Introduction: We investigated the effect of resistance exercise intensity and exercise‐induced metabolic stress on the activation of anabolic signaling and expression of myogenic genes in skeletal muscle. Methods: Ten strength‐trained athletes performed high‐intensity [HI, 74% of 1‐repetition maximum (RM)], middle‐intensity (MI, 54% 1RM), or middle‐intensity (54% 1RM) no‐relaxation exercise (MIR). Kinase phosphorylation level and myogenic gene expression in muscle samples were evaluated before, 45 min, 5 h, and 20 h after exercise. Results: The lactate concentration in MI was approximately 2‐fold lower than in the 2 other sessions, and was highest in MIR. The phosphorylation level of extracellular kinase 1/2 Thr202/Tyr204 after exercise was related to metabolic stress. Metabolic stress induced a decrease in myostatin mRNA expression, whereas mechano‐growth factor mRNA level depended on exercise intensity. Conclusions: This study demonstrates that both intensity and exercise‐induced metabolic stress can be manipulated to affect muscle anabolic signaling. Muscle Nerve 51: 434–442, 2015
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Preliminary evidence for potential differences in intracellular anabolic signaling between loading zones cannot be discounted.
“That said, preliminary evidence for potential differences in intracellular anabolic signaling between loading zones cannot be discounted [40,41], and may have practical implications for RT program design.”
From Loading recommendations for muscle strength, hypertrophy, and local endurance: a re-examination of the repetition continuum. (2021) · cites this paper as [40]
Other research demonstrates divergent responses in intracellular anabolic signaling and myogenic gene expression when training in moderate- (74% to 85% 1RM) and lower (54% to 65% 1RM) loading zones, with selective activation of different kinase pathways between conditions.
“Other research demonstrates divergent responses in intracellular anabolic signaling and myogenic gene expression when training in moderate- (from 74% to 85% 1RM) and lower (from 54% to 65% 1RM) loading zones, with selective activation of different kinase pathways observed between conditions [40,41].”
From Loading recommendations for muscle strength, hypertrophy, and local endurance: a re-examination of the repetition continuum. (2021) · cites this paper as [40]
Acute and longitudinal data suggest a potential hypertrophic benefit to combining loading ranges as part of a structured RT program.
“Both acute [40,41] and longitudinal [83,84,85,86] data suggest a potential hypertrophic benefit to combining loading ranges as part of a structured RT program”
From Loading recommendations for muscle strength, hypertrophy, and local endurance: a re-examination of the repetition continuum. (2021) · cites this paper as [40]
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- Authors
- Popov, D. V., Lysenko, E. A., Bachinin, A. V., Miller, T. F., Kurochkina, N. S., Kravchenko, I. V., Furalyov, V. A., & Vinogradova, O. L.
- Affiliations
- Laboratory of Exercise Physiology Institute of Biomedical Problems, Russian Academy of Sciences 76A Khoroshevskoe Shosse Moscow 123007 Russia
- Russian Academy of Sciences (RU)
- Faculty of Fundamental Medicine M.V. Lomonosov Moscow State University Moscow Russia
- Venue
- Muscle & Nerve
- Publisher
- Wiley
- Published
- 2015-01-06
- Volume
- 51
- Issue
- 3
- Pages
- 434-442
- ISSN
- 0148-639X (ISSN-L)
- 1097-4598
- OpenAlex
- W1907890628
- Cited by
- 32
- References
- 12
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 3 · 2018: 6 · 2017: 3 · 2016: 2 · 2015: 6
- FWCI
- 1.69
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Muscle & Nerve (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Russian Foundation for Basic Research (12-04-01668-)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Keywords
- metabolic stress, anabolic signaling, myogenic gene expression, skeletal muscle, myostatin, mechano growth factor, lactate, strength-trained athletes
- MeSH terms
- Gene Expression Regulation, Humans, Male, Stress, Physiological, Muscle, Skeletal, MAP Kinase Signaling System, Tumor Suppressor Proteins, STAT5 Transcription Factor, Resistance Training, Myostatin, Young Adult
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