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Cited by 67Advanced MRI Techniques and ApplicationsAdvanced NMR Techniques and ApplicationsLiver Disease Diagnosis and Treatment

Quantitative ATP synthesis in human liver measured by localized 31P spectroscopy using the magnetization transfer experiment

Schmid, A. I., Chmelík, M., Szendroedi, J., Krššák, M., Brehm, A., Moser, E., Roden, M.·2008·NMR in Biomedicine
DOI 10.1002/nbm.1207
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Abstract

The liver plays a central role in intermediate metabolism. Accumulation of liver fat (steatosis) predisposes to various liver diseases. Steatosis and abnormal muscle energy metabolism are found in insulin-resistant and type-2 diabetic states. To examine hepatic energy metabolism, we measured hepatocellular lipid content, using proton MRS, and rates of hepatic ATP synthesis in vivo, using the 31P magnetization transfer experiment. A suitable localization scheme was developed and applied to the measurements of longitudinal relaxation times (T1) in six healthy volunteers and the ATP-synthesis experiment in nine healthy volunteers. Liver 31P spectra were modelled and quantified successfully using a time domain fit and the AMARES (advanced method for accurate, robust and efficient spectral fitting of MRS data with use of prior knowledge) algorithm describing the essential components of the dataset. The measured T1 relaxation times are comparable to values reported previously at lower field strengths. All nine subjects in whom saturation transfer was measured had low hepatocellular lipid content (1.5 +/- 0.2% MR signal; mean +/- SEM). The exchange rate constant (k) obtained was 0.30 +/- 0.02 s(-1), and the rate of ATP synthesis was 29.5 +/- 1.8 mM/min. The measured rate of ATP synthesis is about three times higher than in human skeletal muscle and human visual cortex, but only about half of that measured in perfused rat liver. In conclusion, 31P MRS at 3 T provides sufficient sensitivity to detect magnetization transfer effects and can therefore be used to assess ATP synthesis in human liver.

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  • Recent application of 31P NMR to the liver in humans demonstrates that rates of ATP synthesis are 3-fold higher in liver than in muscle.

    “Recent application of 31 P NMR to the liver in humans demonstrates that rates of ATP synthesis are 3-fold higher in liver than in muscle (212).”

    From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [212]

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Authors
Schmid, A. I., Chmelík, M., Szendroedi, J., Krššák, M., Brehm, A., Moser, E., & Roden, M.
Affiliations
  • Hanusch Hospital (AT)
  • Medical University of Vienna (AT)
Corresponding author
Roden, M.
ORCID
Venue
NMR in Biomedicine
Publisher
Wiley
Published
2008-05-28
Volume
21
Issue
5
Pages
437-443
ISSN
  • 0952-3480 (ISSN-L)
  • 1099-1492
DOI
10.1002/nbm.1207
OpenAlex
W1997980906
Cited by
67
References
35
Citations by year
2025: 4 · 2024: 2 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 6 · 2015: 4 · 2014: 7 · 2013: 4 · 2012: 7
FWCI
2.75
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • NMR in Biomedicine (publishedVersion)
  • PubMed (publishedVersion)
Funders
Austrian Science Fund
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
  • Physical Sciences › Chemistry › Spectroscopy › Advanced NMR Techniques and Applications
  • Health Sciences › Medicine › Epidemiology › Liver Disease Diagnosis and Treatment
Keywords
31P NMR, magnetization transfer, liver energy metabolism, steatosis, 1H NMR, T1 relaxation time, exchange rate constants, human liver
MeSH terms
Adenosine Triphosphate, Adult, Brain, Energy Metabolism, Female, Humans, Kinetics, Liver, Male, Magnetic Resonance Spectroscopy, Phospholipids, Phosphorus Isotopes, Protons, Sensitivity and Specificity, Muscle, Skeletal, Hepatocytes, Lipid Metabolism

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