Measurement of intracellular triglyceride stores by H spectroscopy: validation in vivo
Abstract
We validate the use of 1H magnetic resonance spectroscopy (MRS) to quantitatively differentiate between adipocyte and intracellular triglyceride (TG) stores by monitoring the TG methylene proton signals at 1.6 and 1.4 ppm, respectively. In two animal models of intracellular TG accumulation, intrahepatic and intramyocellular TG accumulation was confirmed histologically. Consistent with the histological changes, the methylene signal intensity at 1.4 ppm increased in both liver and muscle, whereas the signal at 1.6 ppm was unchanged. In response to induced fat accumulation, the TG concentration in liver derived from 1H MRS increased from 0 to 44.9 +/- 13.2 micromol/g, and this was matched by increases measured biochemically (2.1 +/- 1.1 to 46.1 +/- 10.9 micromol/g). Supportive evidence that the methylene signal at 1.6 ppm in muscle is derived from investing interfascial adipose tissue was the finding that, in four subjects with generalized lipodystrophy, a disease characterized by absence of interfacial fat, no signal was detected at 1.6 ppm; however, a strong signal was seen at 1.4 ppm. An identical methylene chemical shift at 1.4 ppm was obtained in human subjects with fatty liver where the fat is located exclusively within hepatocytes. In experimental animals, there was a close correlation between hepatic TG content measured in vivo by 1H MRS and chemically by liver biopsy [R = 0.934; P <.0001; slope 0.98, confidence interval (CI) 0.70-1.17; y-intercept 0.26, CI -0.28 to 0. 7…
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Previous studies by our group and others have demonstrated a strong relationship between triglyceride content in liver and muscle and insulin resistance in these tissues.
“Previous studies by our group (9, 17-20) and others (7, 21, 22) have demonstrated a strong relationship between triglyceride content in liver and muscle and insulin resistance in these tissues.”
From Leptin reverses insulin resistance and hepatic steatosis in patients with severe lipodystrophy (2002) · cites this paper as [7]
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- Authors
- Szczepaniak, L. S., Babcock, E. E., Schick, F., Dobbins, R. L., Garg, A., Burns, D. K., McGarry, J. D., & Stein, D. T.
- Affiliations
- Center for Diabetes Research, Departments of
- Radiology,
- University of Tübingen (DE)
- Internal Medicine,
- Pathology, and
- The University of Texas Southwestern Medical Center (US)
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 1999-05-01
- Volume
- 276
- Issue
- 5
- Pages
- E977-E989
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2163769778
- Cited by
- 688
- References
- 1
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 6 · 2023: 16 · 2022: 10 · 2021: 10 · 2020: 16 · 2019: 14 · 2018: 19 · 2017: 29 · 2016: 19 · 2015: 31 · 2014: 40 · 2013: 43 · 2012: 34
- FWCI
- 17.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (7r01dk053358-02)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01-DK-53358-01)
- National Center for Research Resources (P41-RR-02584, P41RR002584)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Health Sciences › Medicine › Epidemiology › Liver Disease Diagnosis and Treatment
- Physical Sciences › Chemistry › Spectroscopy › Advanced NMR Techniques and Applications
- Keywords
- intracellular triglycerides, 1H magnetic resonance spectroscopy, intramyocellular triglyceride, hepatic steatosis, lipodystrophy, noninvasive fat quantification
- MeSH terms
- Adipose Tissue, Adolescent, Adult, Animals, Dogs, Female, Humans, Hydrocarbons, Lipodystrophy, Liver, Male, Methane, Middle Aged, Norepinephrine, Magnetic Resonance Spectroscopy, Obesity, Rabbits, Triglycerides, Muscle, Skeletal
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