CoChat
Sign Up
Cited by 185Listeria monocytogenes in Food SafetyBacterial Genetics and BiotechnologyBiochemical and Structural Characterization

The DOI matches its Crossref record; 63 of the 63 references it cites were found in a registry or an index.

The autolysin Ami contributes to the adhesion of Listeria monocytogenes to eukaryotic cells via its cell wall anchor

Open access
Milohanic, Eliane, Jonquières, Renaud, Cossart, Pascale, Berche, Patrick, Gaillard, Jean‐Louis·2001·Molecular Microbiology
DOI 10.1111/j.1365-2958.2001.02208.x
Open this paper in CoChat — free accountOpen PDF

Abstract

Adherence of pathogenic microorganisms to the cell surface is a key event during infection. We have previously reported the characterization of Listeria monocytogenes transposon mutants defective in adhesion to eukaryotic cells. One of these mutants had lost the ability to produce Ami, a 102 kDa autolytic amidase with an N‐terminal catalytic domain and a C‐terminal cell wall‐anchoring domain made up of repeated modules containing the dipeptide GW (‘GW modules’). We generated ami null mutations by plasmid insertion into L. monocytogenes strains lacking the invasion proteins InlA (EGDΔ inlA ), InlB (EGDΔ inlB ) or both (EGDΔ inlAB ). These mutants were 5–10 times less adherent than their parental strains in various cell types. The adhesion capacity of the mutants was restored by complementation with a DNA fragment encoding the Ami cell wall‐anchoring domain fused to the Ami signal peptide. The cell‐binding activity of the Ami cell wall‐anchoring domain was further demonstrated using the purified polypeptide. Growth of the ami null mutants constructed in EGD and EGDΔ inlAB backgrounds was attenuated in the livers of mice inoculated intravenously, indicating a role for Ami in L. monocytogenes virulence. Adhesive properties have recently been reported in the non‐catalytic domain of two other autolysins, Staphylococcus epidermidis AtlE and Staphylococcus saprophyticus Aas. Interestingly, we found that these domains were also composed of repeated GW modules. Thus, certain autolysins appear to promote bacterial attachment by means of their GW repeat domains. These molecules may contribute to the colonization of host tissues by Gram‐positive bacteria.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1111/j.1365-2958.2001.02208.x

Cites

63 of 63 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Autolysins of Staphylococcus epidermidis affect primary attachment to solid surfaces, and the autolysin of Listeria monocytogenes contributes to adhesion to eucaryotic cells.

    “For example, autolysins of Staphylococcus epidermidis were recently shown to affect primary attachment to solid surfaces, and the autolysin of Listeria monocytogenes contributes to adhesion to eucaryotic cells [44,45].”

    From Positive role of cell wall anchored proteinase PrtP in adhesion of lactococci (2007) · cites this paper as [45]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 1

  1. Ward J.B., 1984, Microbial Cell Wall Synthesis and Autolysis., 159

Cited by

Sorted by citations

Related

Metadata

Authors
Milohanic, E., Jonquières, R., Cossart, P., Berche, P., & Gaillard, J.
Affiliations
  • Inserm (FR)
  • Institut Pasteur (FR)
Corresponding author
Gaillard, Jean‐Louis
ORCID
Venue
Molecular Microbiology
Publisher
Wiley
Published
2001-03
Volume
39
Issue
5
Pages
1212-1224
ISSN
  • 0950-382X (ISSN-L)
  • 1365-2958
DOI
10.1111/j.1365-2958.2001.02208.x
OpenAlex
W2128037586
Cited by
185
References
63
Citations by year
2026: 4 · 2025: 3 · 2024: 3 · 2023: 5 · 2022: 2 · 2021: 5 · 2020: 3 · 2019: 6 · 2018: 4 · 2017: 2 · 2016: 5 · 2015: 3 · 2014: 12 · 2013: 6 · 2012: 9
FWCI
10.92
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 1090 d)
Locations
  • Molecular Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Listeria monocytogenes in Food Safety
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Keywords
Listeria monocytogenes, bacterial adhesion, cell surface binding, integrated nested Laplace approximation, InlB, virulence, complementation
MeSH terms
Amino Acid Sequence, Animals, Bacterial Adhesion, Cell Wall, Eukaryotic Cells, Female, Humans, Listeriosis, Listeria monocytogenes, Molecular Sequence Data, Mutation, N-Acetylmuramoyl-L-alanine Amidase, Tumor Cells, Cultured, Virulence, Sequence Alignment, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review